Horseradish peroxidase-trumpet flower-shaped zinc phytate-glassy carbon electrode electrochemical biosensor and construction method thereof

A technology of horseradish peroxidase and zinc phytate, which is applied in the field of biosensors to achieve the effects of low cost, low detection limit and good bio-affinity

Inactive Publication Date: 2014-04-02
SHANGHAI NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] So far, there is no biosensor for detecting hydrogen peroxide prepared by using the "green economy" morning glory-shaped zinc phytate

Method used

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  • Horseradish peroxidase-trumpet flower-shaped zinc phytate-glassy carbon electrode electrochemical biosensor and construction method thereof
  • Horseradish peroxidase-trumpet flower-shaped zinc phytate-glassy carbon electrode electrochemical biosensor and construction method thereof
  • Horseradish peroxidase-trumpet flower-shaped zinc phytate-glassy carbon electrode electrochemical biosensor and construction method thereof

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Embodiment Construction

[0028] Below in conjunction with specific embodiment, further illustrate the present invention.

[0029] (1) Heat 1mmol / L sodium phytate solution to boiling, then slowly add 6mmol / L ZnCl with the same volume of pH as 2 2 hydrochloric acid solution, reacted at 80-100°C for 1h, cooled to room temperature, and obtained trumpet-shaped zinc phytate (Zn-IP 6 ) solution;

[0030] (2) On the polished glassy carbon electrode (GCE) surface (7mm 2 ) was drip-coated with 3 μL 1 mmol / L zinc phytate solution, and dried in the refrigerator (4°C) to obtain a glassy carbon electrode modified with zinc phytate, which was denoted as Zn-IP 6 / GCE;

[0031] (3) 3 μL of 5mg / mL horseradish peroxidase (HRP) solution (pH=7.0PBS) was fixed on the glassy carbon electrode modified by zinc phytate-shaped zinc phytate by drop coating method, and placed in the refrigerator (4 ℃) to dry, denoted as HRP / Zn-IP 6 / GCE;

[0032] (4) drop the dilution of the phosphate buffer of 3 μ L perfluorosulfonic acid ...

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Abstract

The invention discloses a horseradish peroxidase-trumpet flower-shaped zinc phytate-glassy carbon electrode electrochemical biosensor and a construction method thereof. The horseradish peroxidase-trumpet flower-shaped zinc phytate-glassy carbon electrode electrochemical biosensor utilizes a glassy carbon electrode as a substrate. The substrate is modified by trumpet flower-shaped zinc phytate. Through the trumpet flower-shaped zinc phytate, the glassy carbon electrode is modified by horseradish peroxidase. The outermost layer of the horseradish peroxidase-trumpet flower-shaped zinc phytate-glassy carbon electrode electrochemical biosensor is modified by perfluorosulfonic acid for protecting biological activity of horseradish peroxidase. The horseradish peroxidase-trumpet flower-shaped zinc phytate-glassy carbon electrode electrochemical biosensor can realize direct electron transfer, has short H2O2 response time, a low detection limit, high sensitivity and a small Michaelis constant, can complete accurate and fast determination of H2O2, and has the advantages of environmental protection, low cost and good biocompatibility.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to a horseradish peroxidase-galenia-shaped zinc phytate-glassy carbon electrode electrochemical biosensor and a construction method thereof, mainly using a plant source with excellent biological affinity Bellflower-shaped zinc phytate was used to prepare hydrogen peroxide biosensor. Background technique [0002] Hydrogen peroxide is a very important chemical product with multiple functions such as sterilization, bleaching, oxidation and disinfection. It has a very wide application value, so the determination of hydrogen peroxide content is of great significance in many fields such as biopharmaceuticals, food science, industrial analysis, environmental monitoring and clinical chemistry. The main methods used to detect hydrogen peroxide are fluorescence method, chemiluminescence method, electrochemical method, spectroscopic method and titration method. In recent years, electrochemi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 杨海峰接德丽
Owner SHANGHAI NORMAL UNIVERSITY
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