Method for manufacturing low molecular weight hyaluronic acid

A hyaluronic acid and low-molecular-weight technology, which can be used in medical preparations containing active ingredients, skin care preparations, pharmaceutical formulations, etc., can solve problems such as low quality and increased costs, and achieve the effect of simple procedures

Active Publication Date: 2012-08-08
IL DONG PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The present invention provides a method for reducing the molecular weight of high-molecular hyaluronic acid, which solves the problem of low quality due to impurities generated in the known method and inefficiencies such as increased costs due to the addition of complicated processes, thereby saving high costs. Cost-effective method of producing high-quality low-molecular-weight hyaluronic acid

Method used

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  • Method for manufacturing low molecular weight hyaluronic acid
  • Method for manufacturing low molecular weight hyaluronic acid
  • Method for manufacturing low molecular weight hyaluronic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Method for producing low-molecular-weight hyaluronic acid using CA1 activated carbon

[0061] In this example, the raw material for the production of low-molecular-weight hyaluronic acid used an adjusted substance isolated and purified from a culture obtained by culturing Streptococcus ID9102 (KCTC11395BP) as a production strain, and the average value of hyaluronic acid contained in the raw material was The molecular weight is 3.5 million Da, and the concentration of endotoxin far exceeds 0.5 EU / mg.

[0062] First, the culture conditions are as follows.

[0063] Use ID9102 (KCTC11395BP) in Streptococcus as the production strain of hyaluronic acid, glucose 40-100g / L, yeast X2-5g / L, old peptone 10-20g / L, magnesium sulfonate 0.5-1g / L, monophosphate The aerobic conditions of potassium hydrogen 1-5g / L, sodium chloride 2-10g / L, glutamic acid 0.1-1g / L, pH6.0-7.0, 32-37℃, 0.1-1vvm were carried out in a 75L fermenter to cultivate.

[0064] The basic conditions of the reacti...

Embodiment 2

[0071] Method for producing low-molecular-weight hyaluronic acid using CGSP activated carbon

[0072] The hyaluronic acid sample and reaction conditions used in were used, but Norit CGSP (hereinafter referred to as CGSP) activated carbon was used instead of CA1 activated carbon and a hydrolysis vehicle was used to compare and analyze low molecular weight transparent Acid production capacity.

[0073] The sample collection time is 3 hours, 6 hours, and 18 hours. After the reaction, each sample is taken and analyzed according to the above-mentioned analysis method for endotoxin, hyaluronic acid concentration, and average molecular weight, and the results obtained are shown in [Table 2]. It can be confirmed that as the reaction temperature increases similarly to CA1 activated carbon, the decomposition ability of CGSP activated carbon is improved, and the speed of low molecular weight is accelerated. Under the conditions of 25°C and CGSP 4X, 1 million Da of hyaluronic acid can...

Embodiment 3

[0079] Method for producing low-molecular-weight hyaluronic acid using CASP activated carbon

[0080] The same conditions as those used in were used. A comparative analysis was performed using Norit CASP (hereinafter referred to as CASP) activated carbon instead of CA1 activated carbon.

[0081] The sample collection time was 3 hours, 6 hours, and 18 hours. After the reaction, each sample was taken and analyzed according to the above analysis method for endotoxin, hyaluronic acid concentration, and average molecular weight, and the results as [Table 3] were obtained. It was confirmed that as the reaction temperature increased to be similar to CA1 and CGSP activated carbon, the decomposition power of CASP activated carbon also increased and the speed of low molecular weight accelerated. Under the conditions of 25°C and CASP 4X, hyaluronic acid of 1 million Da can be obtained in 18 hours, and at 35°C, it shows the same level of molecular weight decomposition ability at a con...

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Abstract

The present invention relates to a method for converting high molecular weight hyaluronic acid produced by Streptococcus ID9102 to a low molecular weight hyaluronic acid. More particularly, the present invention relates to a method for uniformly reducing the molecular weight of hyaluronic acid by reacting high molecular weight hyaluronic acid with activated carbon under certain decomposition conditions while retaining the properties of hyaluronic acid.; Compared with the typical methods which require complicated additional processing conditions such as pH treatment, reaction catalysts, and heating, the method for converting high molecular weight hyaluronic acid to low molecular weight hyaluronic acid according to the present invention provides convenient processing by eliminating the inconvenience of retreatment for removing input materials and even having the effect of removing impurities, to conveniently and economically produce high purity, low molecular weight hyaluronic acid. The method of lowering molecular weight according to the present invention is advantageous in that the molecular weight of low molecular weight hyaluronic acid may be variously adjusted by changing reaction conditions using activated carbon.; The low molecular weight hyaluronic acid according to the present invention may be manufactured to comply with pharmaceutical standards as well as with cosmetic or food standards while retaining the properties of hyaluronic acid.

Description

technical field [0001] This application takes priority from Korean Patent Application No. 10-2009-0087185 filed on September 15, 2009, and the above specification is a reference of this application. [0002] The present invention relates to a method for producing low-molecular-weight hyaluronic acid while maintaining the intrinsic properties of high-molecular-weight hyaluronic acid, such as viscosity, elasticity, and moisture absorption. A method for producing high-quality low-molecular-weight hyaluronic acid that is easy to produce and is industrially applicable. Background technique [0003] Hyaluronic acid (Hyaluronic acid (HA), Hyaluronan, (C 14 h 20 NNaO 11 )n (n>1000)) are macromolecules present in all living organisms and are polysaccharides called glycosaminoglycan. is like [ figure 1 ] structure, β-1, 3-bound D-glucuronic acid and N-acetylglucosamine are repeatedly connected in one unit (one unit) to the β-1, 4-bound structure. It is a water-soluble substan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/0072C08B37/0003A61K31/728A61K8/735C08K3/0025A61Q19/08C08K3/04C08B37/003
Inventor 姜大中任种赫康在勋
Owner IL DONG PHARMA CO LTD
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