Method for improving ratio of female fish in cynoglossus semilaevis cultured fingerlings

A technology for semi-smooth tongue sole and female fish, which is applied in fish farming, biochemical equipment and methods, and microbial measurement/inspection, etc. It can solve the problems of low proportion of female fish and low breeding output, so as to improve the proportion of physiological female fish and improve The effect of breeding yield and increasing growth rate

Active Publication Date: 2012-08-15
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for increasing the proportion of female fish in tongue sole cultured fry, so as to

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  • Method for improving ratio of female fish in cynoglossus semilaevis cultured fingerlings
  • Method for improving ratio of female fish in cynoglossus semilaevis cultured fingerlings

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Embodiment Construction

[0029] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0030] 1. Screening of high-quality male broodstock of tongue sole

[0031] 1. Acquisition of sex chromosome-linked microsatellite markers in tongue sole:

[0032] The sex chromosome-linked microsatellite marker sequence used in the present invention is derived from our genome sequencing project on half-smooth tongue sole. The female and male individuals of half-smooth tongue sole were subjected to whole-genome sequencing using SOLEXA sequencing technology. The genome sequences of the female individuals were compared with the genome sequences of the male individuals, and a sex chromosome-specific microsatellite sequence was screened out, named SChen-SSR2. The consensus sequence is SEQ ID NO:1, and the female-specific sequence is SEQ ID NO:2.

[0033] Male and female consensus fragment sequence (274bp): SEQ ID NO: 1

[0034] CAGTGTGTGTTTATT...

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Abstract

The invention relates to a method for improving the ratio of female fish in cynoglossus semilaevis cultured fingerlings. The method comprises the following steps of: 1) screening male parent fish; 2) performing artificial propagation and family establishment; 3) cultivating fish fries with different families and detecting the ratio of male and female fish; and 4) cultivating a high-female and fast-growing family and propagating, wherein a genetic marker used in the step 1) is SChen-SSR2. According to the invention, a method for identifying cynoglossus semilaevis genetic female fish and genetic male fish is established by the SChen-SSR2 marker, and then a method for improving the ratio of female fish in cynoglossus semilaevis cultured fingerlings is established by breeding generations fromthe high-quality normal male fish or establishing a cynoglossus family with a high female ratio bred by the families. The method provided by the invention can be used for solving the most common problems of too high ratio of male fish and too low ratio of physiological female fish in the cultured populations, and thus the method is of great importance in the scale production and cultivation of high-quality cynoglossus semilaevis fingerlings, the improvement of cultivation yield and economic benefit, and the promotion of continuous and rapid development of the cynoglossus semilaevis cultivation industry.

Description

technical field [0001] The invention belongs to the technical field of sex control in seawater fish culture, and in particular relates to a method for increasing the proportion of female fish of tongue sole cultured fry. Background technique [0002] Cynoglossus semiliaevis (Cynoglossus semiliaevis) is a rare and valuable seawater fish endemic to my country. It belongs to warm-water demersal fish in coastal waters. It is distributed in coastal areas of my country, mostly in the Yellow Sea and Bohai Sea. Semi-smooth tongue sole is popular among consumers because of its delicious taste, tender meat and rich nutrition. Its market value is extremely high and its breeding prospect is very broad. As one of the main species of marine fish culture in my country, the annual production of tongue sole has reached more than 10,000 tons. Based on the average market price of 120 yuan / 500 grams, the current annual output value of tongue sole aquaculture has reached more than 2.4 billion yu...

Claims

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Application Information

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IPC IPC(8): A01K61/00C12Q1/68
CPCY02A40/81
Inventor 陈松林胡乔木李仰真梁卓张静刘珊珊杜民邵长伟刘峰
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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