Method for improving ratio of female fish in cynoglossus semilaevis cultured fingerlings
A technology for semi-smooth tongue sole and female fish, which is applied in fish farming, biochemical equipment and methods, and microbial measurement/inspection, etc. It can solve the problems of low proportion of female fish and low breeding output, so as to improve the proportion of physiological female fish and improve The effect of breeding yield and increasing growth rate
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[0029] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.
[0030] 1. Screening of high-quality male broodstock of tongue sole
[0031] 1. Acquisition of sex chromosome-linked microsatellite markers in tongue sole:
[0032] The sex chromosome-linked microsatellite marker sequence used in the present invention is derived from our genome sequencing project on half-smooth tongue sole. The female and male individuals of half-smooth tongue sole were subjected to whole-genome sequencing using SOLEXA sequencing technology. The genome sequences of the female individuals were compared with the genome sequences of the male individuals, and a sex chromosome-specific microsatellite sequence was screened out, named SChen-SSR2. The consensus sequence is SEQ ID NO:1, and the female-specific sequence is SEQ ID NO:2.
[0033] Male and female consensus fragment sequence (274bp): SEQ ID NO: 1
[0034] CAGTGTGTGTTTATT...
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