Combined strain formula for producing grain stillage biological feed through fermenting grain stillage and screening method of the combined strain formula

Abstract

The invention discloses a strain combined formula for producing grain stillage biological ferment feed through fermenting grain stillage and a screening method of the combined strain formula. The strain combined formula comprises a grain stillage culture medium and strains; the formula of the grain stillage culture medium comprises 60 percent of fresh grain stillage, 10 percent of maize flour, 10 percent of rapeseed meal and 20 percent of water; 15mg of ferrous sulfate, 12mg of zinc sulfate, 10mg of manganese sulfate, 15mg of copper sulfate and 2g of calcium hydrogen phosphate are additionally added into each 100g of the grain stillage culture medium; the strains comprise Bacillus subtilis, Trichoderma viride, Lactobacillus acidophilus and Brettanomyces custersianus, the inoculum concentration is 1.5 percent for each strain; and the viable count of Bacillus subtilis is 1.33*10<11>cfu / mL, the viable count of Trichoderma viride is 7.0*10<10>cfu / mL, the viable count of Brettanomyces custersianus is 3.5*10<11>cfu / mL, and the viable count of Lactobacillus acidophilus is 2.1*10<10>cfu / mL.

Description

technical field [0001] The invention relates to a strain combination formula and a screening method for producing distiller's grain biological feed by fermenting distiller's grains. Background technique [0002] The annual output of distiller's grains in my country is as high as more than 21 million tons. Based on the fact that distiller's grains contain certain protein, rich in amino acids, vitamins and various trace elements, etc., the traditional method is to feed fresh distiller's grains directly to livestock as feed materials. There are two methods for this method: There are two disadvantages: first, the crude fiber content in fresh distiller's grains is high, the palatability of livestock is poor, the digestibility is low, and moldy distiller's grains are prone to pests and diseases; second, fresh distiller's grains have high moisture content, are difficult to transport, have high acidity, and are easy to spoil. For this reason, people have carried out various studies o...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a strain combination formula and screening method for effectively fermenting distiller's grains to produce distiller's grains biological feed, so as to overcome the shortcomings of the prior art such as large energy consumption and insufficient conversion of nutrients

Images