Unlock instant, AI-driven research and patent intelligence for your innovation.

Primer for detecting reverse transcription fluorescence PCR (polymerase chain reaction) of salmonella typhi

A Salmonella typhi, reverse transcription technology, applied in the direction of microbial determination/testing, resistance to vector-borne diseases, biochemical equipment and methods, etc., can solve the problems of clinical diagnosis and treatment difficulties, atypical clinical manifestations, etc.

Active Publication Date: 2014-04-30
ICDC CHINA CDC
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the widespread use of antibiotics and the influence of many factors such as the pathogen's own drug resistance and mutation, the recurrence rate of typhoid fever has increased in recent years, and the clinical manifestations are very atypical, which brings great difficulties to early clinical diagnosis and treatment. Therefore, it is necessary to establish a rapid and accurate molecular biological detection method for Salmonella typhi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer for detecting reverse transcription fluorescence PCR (polymerase chain reaction) of salmonella typhi
  • Primer for detecting reverse transcription fluorescence PCR (polymerase chain reaction) of salmonella typhi
  • Primer for detecting reverse transcription fluorescence PCR (polymerase chain reaction) of salmonella typhi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0014] Example Design and detection method of primers for reverse transcription fluorescent PCR detection of Salmonella typhi

[0015] 1 Materials and methods

[0016] 1.1 Experimental strains

[0017] Salmonella typhi 64 strains used in the present embodiment, Salmonella typhi type A, type B and type C and other 31 kinds of non-typhoid Salmonella serotypes total 57 strains (Table 1), all serotypes use Salmonella anti- Serum (purchased from Statens Serum Institut, SSI, Denmark) was used for serotyping. In addition, other common intestinal pathogens and Streptococcus pneumoniae, Borrelia burgdorferi, Leptospira, Legionella pneumophila, Neisseria meningitidis, Rickettsia, Brucella, etc. Bacteria and other bacterial strains (Table 1) were used for specificity and sensitivity evaluation, and the above-mentioned bacterial strains were all from the Institute of Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention.

[0018] 1.2 Primer design...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a primer for detecting reverse transcription fluorescence PCR (polymerase chain reaction) of salmonella typhi, which comprises 5'-GACTTGCCTTTAAAAGATACCA-3' and 5'-AGAGTGCGTTTGAACACTT-3'. The invention further provides a kit which contains the primer and is used for detecting the salmonella typhi. The primer is a specificity primer which is designed for STY1381 genes of the salmonella typhi. In the reverse transcription fluorescence PCR for detecting the target genes, 64 types of salmonella typhi detected by the method are positive, amplification results of other 34 types of non-salmonella typhi blood serum, other 5 types of pathogenic entero bacteria causing diarrhea and 8 types of common non-salmonella mainly causing fever are negative, accordingly, the method is high in specificity and sensitivity, and lower limit of detection of the reverse transcription fluorescence PCR is 5pg / reaction.

Description

technical field [0001] The invention relates to the detection of Salmonella typhi, in particular to primers for reverse transcription fluorescent PCR detection of Salmonella typhi. Background technique [0002] Salmonella typhi (S.Typhi) is one of the most common pathogens in intestinal infectious diseases. Rapid and accurate detection of Salmonella typhi is an important means to control the epidemic of typhoid fever. At present, the standard method for detecting typhoid fever in the laboratory is blood culture, which is time-consuming (3-5 days) and labor-intensive, and the detection rate is easily affected by factors such as the course of the disease, the amount of blood collected, and whether the patient is taking antibiotics. It is difficult for isolation and culture to meet the fast and accurate requirements. Serological diagnosis has the problems of low specificity and poor accuracy. Clinically, it is difficult to distinguish between typhoid and paratyphoid based on sy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCY02A50/30
Inventor 闫梅英樊粉霞阚飙
Owner ICDC CHINA CDC