Application of additive in enhancing oxidization of arsenical gold mineral by bacteria
A technology of bacterial oxidation and additives, applied in the direction of improving process efficiency, etc., can solve the problems of bacterial poisoning and affecting the normal reproduction and growth of bacteria, and achieve the effect of strengthening bacterial oxidation, low cost and remarkable effect
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Embodiment 1
[0014] Embodiment 1: in 250mL Erlenmeyer flask, add 150mL9K substratum and add the arsenopyrite of 5.0g, then inoculate 10mL of acidophilic Thiobacillus ferrooxidans (bacteria adopt common acidophilic ferrous oxide for leaching ore Thiobacillus, cultivated using conventional 9k medium, the number of bacteria reached 10 after mass reproduction 8~9 individual / ml), adjust the pH of the pulp to 1.8. Cultivate at a constant temperature of 30° C. in an air bath shaker, the shaker rotates at a speed of 160 r / min, and the additive (ammonium persulfate) is added while the bacteria are inoculated, and the stirring oxidation time is 144 hours. When the addition amount of the additive is 20% of the total weight of the arsenic-containing gold ore, the arsenic oxidation rate of the arsenic-containing gold ore is 40.4%, and the conversion rate of As(III) is 30.41%.
Embodiment 2
[0015] Example 2: Add 150 mL of 9K medium and 5.0 g of arsenopyrite to a 250 mL Erlenmeyer flask, then inoculate 10 mL of the same acidophilic Thiobacillus ferrooxidans as in Example 1, and adjust the pH of the slurry to 1.8. Cultivate at a constant temperature of 30° C. in an air bath shaker, the shaker speed is 160 r / min, the additive (ammonium persulfate) is added while the bacteria are inoculated, and the stirring oxidation time is 120 h. When the additive amount is 50% of the total arsenic-containing gold ore, the arsenic oxidation rate of the arsenic-containing gold ore is 95%, and the conversion rate of As(III) is 38.43%.
Embodiment 3
[0016] Example 3: Add 150 mL of 9K medium and 5.0 g of arsenopyrite to a 250 mL Erlenmeyer flask, then inoculate 10 mL of the same acidophilic Thiobacillus ferrooxidans as in Example 1, and adjust the pH of the slurry to 1.8. Cultivate at a constant temperature of 30°C in an air bath shaker, the shaker rotates at a speed of 160r / min, add the additive (ammonium persulfate) after bacterial leaching for 48 hours, and continue leaching for 120 hours. When the additive amount is 20% of the total amount of arsenic-containing gold ore, the oxidation rate of arsenic in the arsenic-containing gold ore is 35.1%, and the conversion rate of As(III) is 48.8%.
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