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Method for detecting a compound preparation of green tea of radix sophorae flavescentis

A technology of compound preparation and Sophora flavescens green tea, which is applied in the field of traditional Chinese medicine, can solve the problems of unsatisfactory content control of finished preparations, unfavorable drug safety for patients, poor method stability and reproducibility, etc.

Active Publication Date: 2012-08-15
WUHAN HUADA PHARMA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the detection and identification of Sophora flavescens green tea ointment in this invention is to adopt thin-layer chromatography (TLC) to carry out identification to Sophora flavescens crude extract, green tea extract in the Sophora flavescens green tea composition, the content control of finished preparation is not enough Ideal, the stability and reproducibility of the method are poor, which will affect the test results of the preparation
Moreover, in this test, the content determination and content control of various active ingredients in the preparation have not been carried out, which is not conducive to the production and quality control of the product, and is not conducive to the drug safety of patients.

Method used

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  • Method for detecting a compound preparation of green tea of radix sophorae flavescentis
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  • Method for detecting a compound preparation of green tea of radix sophorae flavescentis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 prepares Sophora flavescens green tea ointment

[0037] Grind the traditional Chinese medicine Sophora flavescens, add 0.2% HCl to wet it for 30 minutes, put it into the percolation cylinder, press it tightly, slowly pour the above liquid until the cross section of the medicine powder is covered, soak for 1 hour, and start percolation, the flow rate is 800-1000ml / cm 2 per hour, when the percolation liquid has no obvious orange-red precipitation, the percolation ends. Let the percolation liquid stand still for 24 hours, siphon the supernatant, and pass through the strong acid cationic resin column with a flow rate of 500-800ml / cm 2 / hour, stop the exchange when orange-red precipitate appears in the exchange solution. Pour out the resin, rinse it with water, dry it by centrifugation, dry the resin at 40 degrees Celsius, add an appropriate amount of concentrated ammonia water to alkalinize it, put it into a Soxhlet extractor, and add chloroform to reflux f...

Embodiment 2

[0038] Example 2: Determination of the content of epigallocatechin gallate in Sophora flavescens green tea ointment by high performance liquid chromatography

[0039] 1. Instruments and materials

[0040] High-performance liquid chromatography (Shimadzu LC-10ATvp type), ultraviolet detector; UV-visible spectrophotometer (Shimadzu MV-2501PC type); electronic analytical balance (SARTORIMS BP211D),

[0041] Sophora flavescens green tea ointment (preparation of 3 batches of flavescens green tea ointment according to the method described in Example 1, marking batch numbers respectively: 110702, 110804, 110901),

[0042] Reference substance: epigallocatechin gallate, purchased from Sigma Company, purity ≥ 98%,

[0043] Methanol (chromatographically pure, Tianjin Concord Technology Co., Ltd.), acetonitrile (chromatographically pure, DIKMA Company), Milli-Q ultrapure water, and other reagents were analytically pure.

[0044] 2. Chromatographic conditions:

[0045] Stationary phase:...

Embodiment 3

[0082] Embodiment 3: Determination of content of matrine and oxymatrine in Sophora flavescens green tea ointment by high performance liquid chromatography

[0083] 1. Instruments and materials

[0084] High-performance liquid chromatography (Shimadzu LC-10ATvp type), ultraviolet detector; UV-visible spectrophotometer (Shimadzu MV-2501PC type); electronic analytical balance (SARTORIMS BP211D)

[0085] Sophora flavescens green tea ointment (3 batches of flavescens green tea ointment were prepared according to the method described in Example 1, and the batch numbers were respectively marked: 110702, 110804, 110901)

[0086] Reference substances: matrine and oxymatrine were purchased from China National Institutes for Food and Drug Control.

[0087] Methanol (chromatographically pure, Tianjin Concord Technology Co., Ltd.), acetonitrile (chromatographically pure, DIKMA Company), Milli-Q ultrapure water, and other reagents were analytically pure.

[0088] 2. Chromatographic condit...

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Abstract

The invention provides a method for detecting a compound preparation of green tea of radix sophorae flavescentis. The method comprises the following steps of extracting active constituents from the compound preparation of the green tea of the radix sophorae flavescentis, and detecting by utilizing a high performance liquid chromatography so as to determine the content of the active constituents, wherein the active constituents are at least one kind of epigallocatechin gallate, oxymatrine and matrine. With the adoption of the method, the active constituents of the compound preparation of the green tea of the radix sophorae flavescentis can be effectively detected.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine. Specifically, the present invention relates to a method for detecting a compound preparation of Sophora flavescens and green tea. Background technique [0002] Chinese patent publication CN1239669A has reported Sophora flavescens green tea composition and its application in the treatment of genital warts. The composition is formulated into tan ointment from Sophora flavescens crude extract, green tea crude extract and excipients, and is used to treat human nipple Genital warts caused by HPV infection. The compound preparation of sophora flavescens and green tea disclosed by CN1239669A belongs to the traditional Chinese medicine preparation, has no toxic and side effects on human body, and has simple and reasonable preparation process. But the detection and identification of Sophora flavescens green tea ointment in this invention is to adopt thin-layer chromatography (TLC) to carry ou...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/36
Inventor 黄璐陈顺钦张思佳牟峰杨焕明
Owner WUHAN HUADA PHARMA