In-vitro culture and planting regeneration and propagation method of Xianglei honeysuckle leaves and culture medium

A technology for in vitro culture and rooting medium, which is applied in the field of in vitro culture of Xianglei honeysuckle leaves, plant regeneration and propagation methods and medium, can solve the problems of less rooting, low proliferation coefficient and high cost of plants, and achieves seedling growth. Robust, high multiplication factor, cost-saving effect

Inactive Publication Date: 2012-09-12
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims to overcome the low multiplication coefficient existing in the in vitro cultivation and multiplication technology of Xianglei honeysuckle, the obtained plants have few roots, take a long time, high cost, low quality, low t

Method used

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  • In-vitro culture and planting regeneration and propagation method of Xianglei honeysuckle leaves and culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Induction of leaf callus:

[0016] Select the young leaves or adult leaves on the healthy plants of Xianglei honeysuckle, soak them in 75% alcohol for 30 seconds, then disinfect them with 2% NaClO for 6 minutes, rinse them with sterile water for 3 times, and put the leaves on the ultra-clean workbench cut into 1cm 2 A small piece was inoculated into the medium composition B 5 +6-BA 1.0mg / L+NAA 0.1mg / L+3% sucrose in callus induction medium for 30-40 days;

[0017] Differentiation of callus:

[0018] Transfer the induced callus to medium composition B 5 +6-BA 1.5mg / L+NAA 0.1mg / L+3% sucrose in the differentiation medium for 1 to 2 months to get clustered buds;

[0019] Strong seedling cultivation:

[0020] Separate the dense clustered buds and transfer them to hormone-free 1 / 2MS strong seedling medium for 1-2 weeks;

[0021] Rooting culture:

[0022] Transfer the rootless strong seedlings to the rooting medium whose medium composition is 1 / 4MS+NAA0.2mg / L+IBA0.4mg / L+...

Embodiment 2

[0023] Embodiment 2 Comparison experiment of the effect of Xianglei honeysuckle leaf in vitro culture and plant regeneration and multiplication method of the present invention and prior art

[0024] Under the premise that other conditions are consistent, the method of the present invention is compared with the in vitro culture method in the prior art, and the experimental parameters and results are as shown in Table 1:

[0025] Table 1

[0026]

[0027] As can be seen from Table 1, compared with the existing Xianglei honeysuckle in vitro tissue culture technology, using the method and medium of the present invention to carry out in vitro culture and regeneration of Xianglei honeysuckle, the proliferation coefficient is high, and the callus The differentiation rate reaches 70% to 80%; 11-13 rootless seedlings take root after 20-25 days of rooting culture, which takes a short time and the seedlings grow robustly, and the concentrations of hormones, sugars, and MS mother liquo...

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Abstract

The invention discloses an in-vitro culture and planting regeneration and propagation method of Xianglei honeysuckle leaves and a culture medium. When being used for culturing Xianglei honeysuckle, the method and the culture medium provided by the invention can effectively solve the problems that in the existing in-vitro culture and propagation technology of the Xianglei honeysuckle, the propagation factor is low, the rooting of the obtained strains is little, the required time is long, the cost is high, the quality is low, the transplanting survival rate is low, and the market requirement onhigh-quality seedlings is difficult to meet.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a method for in vitro cultivation of honeysuckle leaves of Xianglei, a plant regeneration and multiplication method and a culture medium. Background technique [0002] The original plant of Xianglei honeysuckle is Lonicera macranthoides Hand Mazz, which was bred in 1997 from a natural variant of Lonicera macranthoides. [0003] In production, the main propagation methods of Xianglei honeysuckle are cutting and grafting, but the rooting time of cutting propagation is longer (1-3 months), and the rooting rate is not high (only 30% to 40%). "Xianglei honeysuckle", after the tree crown is expanded, it is prone to late incompatibility and premature aging, most of them turn yellow, some wither or die, and the reproduction coefficient and reproduction speed of the two methods are limited, while the existing gray felt wool Lonicera in vitro tissue culture technolog...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 于晓英韩蓉符红艳廖祯妮陈己任
Owner HUNAN AGRICULTURAL UNIV
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