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Triene antibiotic, preparation method thereof and application thereof

A technology of antibiotics and fermented liquid, which is applied in the biological field to achieve the effects of easy decomposition, reduced decomposition degree and strong antibacterial effect

Inactive Publication Date: 2014-07-16
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, although there are many kinds of drugs for antifungal infection clinically, most chemically synthesized drugs can only be used for the treatment of superficial infections, and very few of them can be used as internal medicines.

Method used

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  • Triene antibiotic, preparation method thereof and application thereof
  • Triene antibiotic, preparation method thereof and application thereof
  • Triene antibiotic, preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation method of triene antibiotic:

[0048] (1) Scrape the bacteria from the mature slant of Streptomyces Shandong and inoculate it in Gaoshi No. 1 liquid medium, shake the flask at 28°C (90r.min -1 ) was cultured for 72 hours, and the liquid volume in the Erlenmeyer flask was 20%. Then the seed liquid is transferred to (5L) fermentor with 10% inoculum size, 28 ℃ of temperature, initial stirring speed is 200r.min -1 , after 5 hours of inoculation, adjust the stirring speed to 300r.min -1 18 hours after inoculation, adjust the stirring speed to 400r.min -1 and keep it; after 5 days, the fermentation is over, and the obtained fermentation liquid is 6000r.min at 4°C -1 Centrifuge for 20 minutes to remove bacteria, adjust the pH of the obtained supernatant to pH = 3 and then 12000r.min -1 Centrifuge for 15 minutes, keep the supernatant, adjust the pH of the supernatant back to pH=6, and store it at 4°C for later use;

[0049] (2) Pretreatment with macroporous...

Embodiment 2

[0054] The preparation method of triene antibiotic:

[0055] (1) Scrape the bacteria from the mature slant of Streptomyces Shandong and inoculate it in Gaoshi No. 1 liquid medium, shake the flask at 28°C (90r.min -1 ) were cultured for 72 hours, and the liquid volume in the Erlenmeyer flask was 20%. Then the seed liquid is transferred to (5L) fermenter with 10% (about 400ml) inoculum size, temperature 28 ℃, initial stirring speed is 200r.min -1 , after 5 hours of inoculation, adjust the stirring speed to 300r.min -1 18 hours after inoculation, adjust the stirring speed to 400r.min -1 and keep it; after 5 days, the fermentation is over, and the obtained fermentation liquid is 6000r.min at 4°C -1 Centrifuge for 20 minutes to remove bacteria, adjust the pH of the obtained supernatant to pH = 3 and then 12000r.min -1 Centrifuge for 15 minutes, keep the supernatant, adjust the pH of the supernatant back to pH=6, and store it at 4°C for later use;

[0056] (2) Pretreatment with...

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Abstract

The invention relates to a triene antibiotic. The formula of the triene antibiotic is C23H34O8S and the structural formula of the triene antibiotic is as follows. The preparation method comprises steps of using Shandong streptomycetes to cultivate a fermentation broth, performing centrifugation for the fermentation broth, removing thallus, reserving supernatant liquor, preprocessing the supernatant liquor by using a macroporous resin X-5 column chromatography, preprocessing the supernatant liquor by using a macroporous resin HP-20 column chromatography, performing High Performance Liquid Chromatography (HPLC) semi-preparation liquid phase separation, performing freezing and drying after rotating, evaporating and concentrating collection liquid corresponding to peaks of active constituents, and obtaining required purified products. Accordingly, products which have high purity and little impurity can be obtained. Under the same concentration, the triene antibiotic has a large-diameter inhibition zone and good inhibitory effects.

Description

technical field [0001] The invention relates to an antibiotic, in particular to a triene antibiotic and its preparation method and application, belonging to the field of biotechnology. Background technique [0002] Plant diseases have always been the enemy of agricultural production. Among the three types of plant diseases, the plant diseases caused by pathogenic fungi rank first (fungal diseases, bacterial diseases and viral diseases). Among the more than 200 diseases of wheat, fungal diseases Occupy about 90%, so the loss caused can account for 10%-20% of its total output every year. For a long time, people have used a large amount of chemical pesticides to fight against diseases, which has led to three major problems: polluting the environment, accumulating residual toxins, and ultimately endangering human health; the drug resistance of pathogenic fungi has exploded; beneficial microorganisms have been killed , Destroying the balance of microbial populations in animals a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D309/32C12P17/06A01P3/00C12R1/465
Inventor 李德舜马井玉陈琦
Owner SHANDONG UNIV
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