Triene antibiotic, preparation method thereof and application thereof
A technology of antibiotics and fermented liquid, which is applied in the biological field to achieve the effects of easy decomposition, reduced decomposition degree and strong antibacterial effect
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Embodiment 1
[0047] The preparation method of triene antibiotic:
[0048] (1) Scrape the bacteria from the mature slant of Streptomyces Shandong and inoculate it in Gaoshi No. 1 liquid medium, shake the flask at 28°C (90r.min -1 ) was cultured for 72 hours, and the liquid volume in the Erlenmeyer flask was 20%. Then the seed liquid is transferred to (5L) fermentor with 10% inoculum size, 28 ℃ of temperature, initial stirring speed is 200r.min -1 , after 5 hours of inoculation, adjust the stirring speed to 300r.min -1 18 hours after inoculation, adjust the stirring speed to 400r.min -1 and keep it; after 5 days, the fermentation is over, and the obtained fermentation liquid is 6000r.min at 4°C -1 Centrifuge for 20 minutes to remove bacteria, adjust the pH of the obtained supernatant to pH = 3 and then 12000r.min -1 Centrifuge for 15 minutes, keep the supernatant, adjust the pH of the supernatant back to pH=6, and store it at 4°C for later use;
[0049] (2) Pretreatment with macroporous...
Embodiment 2
[0054] The preparation method of triene antibiotic:
[0055] (1) Scrape the bacteria from the mature slant of Streptomyces Shandong and inoculate it in Gaoshi No. 1 liquid medium, shake the flask at 28°C (90r.min -1 ) were cultured for 72 hours, and the liquid volume in the Erlenmeyer flask was 20%. Then the seed liquid is transferred to (5L) fermenter with 10% (about 400ml) inoculum size, temperature 28 ℃, initial stirring speed is 200r.min -1 , after 5 hours of inoculation, adjust the stirring speed to 300r.min -1 18 hours after inoculation, adjust the stirring speed to 400r.min -1 and keep it; after 5 days, the fermentation is over, and the obtained fermentation liquid is 6000r.min at 4°C -1 Centrifuge for 20 minutes to remove bacteria, adjust the pH of the obtained supernatant to pH = 3 and then 12000r.min -1 Centrifuge for 15 minutes, keep the supernatant, adjust the pH of the supernatant back to pH=6, and store it at 4°C for later use;
[0056] (2) Pretreatment with...
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