Ultrasonic low frequency-high frequency coupled atomization biological reaction device

Abstract

The invention relates to an ultrasonic low frequency-high frequency coupled atomization biological reaction device. The device comprises a reactor tank system, a high-frequency ultrasonic atomization system and a low-frequency ultrasonic generation system, wherein the reactor tank system comprises a reactor tank (8) with a plurality of low-frequency ultrasonic vibrator bonding planes hierarchically arranged at different heights of the reactor tank (8); the low-frequency ultrasonic generation system comprises a plurality of low-frequency ultrasonic vibrators (11), and the low-frequency ultrasonic vibrators are fixed on the low-frequency ultrasonic vibrator bonding planes of the reactor tank (8) through a combination method of central bolts and gluing; and the high-frequency ultrasonic atomization system comprises a ultrasonic atomization cavity (1), wherein the ultrasonic atomization cavity is communicated with the reactor tank (8) through a droplet-flow center pipe (52), and a coupling of the ultrasonic low frequency-high frequency is realized. The device solves the amplification problem of the atomization reactor, and accelerates the update of material surface liquid films.

Description

technical field [0001] The invention relates to the field of bioreactors, in particular to an ultrasonic low-frequency-high-frequency coupled atomization bioreactor. Background technique [0002] Ultrasonic atomization is a major feature of high-frequency sound waves. This type of ultrasonic wave resonates with the atomized sheet, causing the liquid above the atomized sheet to form high-frequency oscillations. Ultrafine particles form water mist. High-frequency ultrasonic waves can make the liquid form tiny water mist, which can provide nutrients for the culture system in cell and tissue culture. Compared with nozzle atomization, the diameter of droplets formed by ultrasonic atomization is smaller (1-10 μm). In the process of biological cell or tissue culture, the smaller the droplet diameter, the larger the gas-liquid specific surface area of ​​the culture medium, which is more conducive to the transfer of oxygen into the liquid. Oxygen-enriched droplets adhere to the su...

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Problems solved by technology

[0005] The purpose of the present invention is to solve the mass transfer resistance caused by the easy formation of a liquid retention layer on the surface of the culture material in the process of cultivating cells / tissues / organs in the atomization bioreactor, which hinders the growth and metabolism of the culture material, and the atomization amount is small and the atomization To deal with problems such as difficulty in reactor amplification, large escape of nutrient mist droplets, and loss of culture medium, an ultrasonic low-frequency-high-frequency coupled atomization bioreactor is provided.

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