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Method for preparing phosphatidylserine

A technology of phosphatidylserine and serine, applied in fermentation and other directions, can solve the problems of low efficiency of enzymatic catalytic reaction and many by-products of phosphatidic acid, and achieve the effects of green process, improved enzymatic conversion efficiency and good product quality.

Inactive Publication Date: 2012-09-19
ACAD OF STATE GRAIN ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method is a phosphatidyl transfer reaction completed in a homogeneous system, which overcomes the problems of more by-product phosphatidic acid and low enzyme-catalyzed reaction efficiency in the prior art

Method used

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  • Method for preparing phosphatidylserine
  • Method for preparing phosphatidylserine

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Experimental program
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Effect test

Embodiment 1

[0023] Phosphatidylcholine (99%, Sigma-Aldrich Company) and L-serine (commercially available) with a molar ratio of 1:9, and γ-valerolactone (Sigma-Aldrich Company) 5 times based on the mass of the reactant, Phospholipase D (derived from Streptomyces, Japan Asahi Kasei Co., Ltd.) based on 5% of the mass of the reactant was put into a biochemical reactor suitable for biological enzyme catalyzed reactions and mixed evenly, and the temperature was controlled at 65 o C, after 2 hours of reaction, the yield of phosphatidylserine reached 98%.

Embodiment 2

[0025] Phosphatidylethanolamine (98%, Sigma-Aldrich Company) and L-serine (commercially available) with a molar ratio of 1:6, and 2-methyltetrahydrofuran (Sigma-Aldrich Company) based on 1 times the mass of reactants, based on Phospholipase D (derived from Streptomyces, Asahi Kasei Co., Ltd., Japan) with 1% of the reactant mass was put into a biochemical reactor suitable for biological enzyme catalyzed reactions and mixed evenly, and the temperature was controlled at 45 o C, after 8 hours of reaction, the yield of phosphatidylserine reached 95%.

Embodiment 3

[0027] Phosphatidylinositol (50%, Sigma-Aldrich Company) and L-serine (commercially available) with a molar ratio of 1:3, and a mixture of γ-valerolactone and 2-methyltetrahydrofuran based on 8 times the mass of the reactant (Volume ratio 1:1, Sigma-Aldrich Company), phospholipase D (derived from Streptomyces, Sigma-Aldrich Company) based on the mass of reactants 10%, put together into any biochemical reactor suitable for biological enzyme-catalyzed reactions Mix well and keep the temperature at 35 o C, after 48 hours of reaction, the yield of phosphatidylserine reached 82%.

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Abstract

The invention discloses a method for preparing phosphatidylserine. According to the method, phospholipid and L-serine are subjected to phospholipid translating acylation reaction under the action of phospholipase D to generate the phosphatidylserine, wherein the phospholipid translating acylation reaction is carried out in a homogeneous medium system and the yield of the phosphatidylserine reaches 80-98 percent. The method disclosed by the invention has the advantages that due to the adoption of a green reaction medium, a reaction system is a homogeneous medium system. No phosphatidic acid as a byproduct is generated, high catalytic efficiency of enzyme and favorable quality of products are obtained and the process is safe and environment-friendly.

Description

technical field [0001] The invention relates to a method for preparing phosphatidylserine. Background technique [0002] Phospholipids are a general term for a class of phosphate-containing lipids, mainly including phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidic acid, and sphingomyelin. Among them, phosphatidylserine (Phosphatidylserine, PS) is an important rare phospholipid, which can affect the transmission of chemical information in the brain and help brain cells store and read data. It is an important nutrient for maintaining normal memory, response and healthy mood of the brain element, known as the "brain vitamin". Its structural formula is as shown: Glycerol is the main skeleton, the 1st and 2nd carbon atoms are connected to fatty acids; the 3rd carbon atom is connected to a phospholipid group with serine. [0003] [0004] Phosphatidylserine [0005] Studies in recent years have shown that PS has the functio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/06
Inventor 段章群王瑛瑶马榕胡飞栾霞杜传林
Owner ACAD OF STATE GRAIN ADMINISTRATION
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