Method for preparing high-purity galacto-oligosaccharide by continuous simulated moving bed chromatography separation

A galacto-oligosaccharide and simulated moving bed technology, which is applied in the field of functional sugar preparation, can solve the problems of low purity and achieve high separation efficiency, extensive health care and medicinal value, and good product quality.

Inactive Publication Date: 2012-09-19
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the production problems such as the low purity of the existing galacto-oligosaccharide final product, the present invention provides a ten-bed continuous simulated moving bed chromatogra

Method used

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  • Method for preparing high-purity galacto-oligosaccharide by continuous simulated moving bed chromatography separation
  • Method for preparing high-purity galacto-oligosaccharide by continuous simulated moving bed chromatography separation
  • Method for preparing high-purity galacto-oligosaccharide by continuous simulated moving bed chromatography separation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Strain BLB-21 was inoculated with the seed solution with a glucose concentration of 300g / L for expansion. The secondary seed solution (concentration: 0.52% of the wet weight of mycelium) was added to 20% lactose solution in an amount of 10% (V / V) for fermentation, adjusted to pH 5.5, and fermented at 25-30° C. for 40 hours. The mixture of sugar components was preliminarily obtained by ultrafiltration. After decolorization by activated carbon, strong acidic cations-weak basic anions-strong acidic cation exchange resins were used for separation and desalination at a temperature of 30-35°C, an injection volume of 10L / h, and a resin height of 1m. A sugar solution with a galacto-oligosaccharide content of 55.24% was detected by HPLC. At this time, the sugar components are glucose, galactose, lactose, and galactooligosaccharides.

[0031] The chromatographic separation parameters are: the filling height of the resin column is 0.65m, the rotating speed of the distribution pla...

Embodiment 2

[0036] Strain BLB-21 was inoculated with the seed solution with a glucose concentration of 400g / L for expansion. The secondary seed solution (concentration: 0.45% of the wet weight of mycelium) was added to 30% lactose solution in an amount of 10% (V / V) for fermentation, adjusted to pH 5.5, and fermented at 30-35° C. for 30 hours. The mixture of sugar components was preliminarily obtained by ultrafiltration. After decolorization by activated carbon, strong acidic cation-weak basic anion-strong acidic cation exchange resin was used for separation and desalination at a temperature of 35-40°C, an injection volume of 10L / h, and a resin height of 1m. A sugar solution with a galacto-oligosaccharide content of 56.54% was detected by HPLC. At this time, the sugar components are glucose, galactose, lactose, and galactooligosaccharides.

[0037] The parameters are: the filling height of the resin column is 0.65m, the rotation speed of the distribution plate is 3° / min, the feed concentr...

Embodiment 3

[0041]The strain BLB-21 was inoculated with the seed solution with a glucose concentration of 500g / L for expansion. The secondary seed solution (concentration: 0.56% of the wet weight of mycelia) was added to 40% lactose solution in an amount of 10% (V / V) for fermentation, adjusted to pH 5.5, and fermented at 35-40° C. for 20 hours. The mixture of sugar components was preliminarily obtained by ultrafiltration. After decolorization by activated carbon, strong acidic cation-weak basic anion-strong acidic cation exchange resin was used for separation and desalination at a temperature of 45-50°C, an injection volume of 10L / h, and a resin height of 1m. A sugar solution with a galacto-oligosaccharide content of 55.72% was detected by HPLC. At this time, the sugar components are glucose, galactose, lactose, and galactooligosaccharides.

[0042] The parameters are: the filling height of the resin column is 0.65m, the rotation speed of the distribution plate is 3° / min, the feed concen...

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Abstract

The invention relates to a production method for a high-purity galacto-oligosaccharide. A galacto-oligosaccharide fermentation broth is prepared by a ten-bed continuous simulated moving bed chromatography separation after ceramic membrane ultrafiltration and ion exchange treatment. The product has a purity of more than 95%, a complete separation of monosaccharide and lactose in a galacto-oligosaccharide solution is well realized with high separation efficiency, and the quality of the product is further improved. The bacterial strain of aspergillus oryzae BLB-21 applied by the product process is preserved in China Center of Industrial Culture Collection on 17th Martch, 2009, with a preservation number of CGMCC No. 2951.

Description

technical field [0001] The invention relates to the field of preparation of functional sugars, in particular to a preparation method of high-purity galacto-oligosaccharides. Background technique [0002] Galacto-oligosaccharides (GOS) is a kind of non-digestible oligosaccharides with natural properties, which is abundant in human breast milk, and the establishment of bifidobacteria in infants is largely dependent on breast milk The galacto-oligosaccharide component in. Compared with other non-digestible oligosaccharides, galacto-oligosaccharides have the advantages of specifically promoting the proliferation of bifidobacteria in the intestinal tract, high safety, and stable heat treatment for food processing. , health products and other aspects have a wide range of applications, the production and application of galacto-oligosaccharides has great potential in the field of food industry. At present, the global manufacturers of galacto-oligosaccharides are mainly in Japan, a...

Claims

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Application Information

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IPC IPC(8): C12P19/00C07H3/06C07H1/08C12R1/69
Inventor 刘宗利王乃强袁卫涛栾庆民冯志臣滕慧李庆华李双茹
Owner BAOLINGBAO BIOLOGY
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