Method for decolorizing silkworm chrysalis protein enzymatic hydrolysis peptide

A technology of proteolysis and anti-oxidation peptides, which is applied in the field of decolorization of silkworm chrysalis proteolysis anti-oxidation peptides, can solve the problems of difficult color, loss of active peptide activity, high protein loss rate, etc., and achieves mild treatment process, easy operation and simple process Effect

Inactive Publication Date: 2012-09-26
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the silkworm chrysalis protein hydrolyzate is dark brown, and it is not easy to be accepted by people as a medicine or food additive, so it must be decolorized.
At present, the decolorization methods of silkworm chrysalis enzymatic hydrolyzate or other active peptides mainly use activated carbon method, hydrogen peroxide method, and organic solvent method, but these methods have problems such as high protein loss rate and serious loss of active peptide activity.

Method used

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  • Method for decolorizing silkworm chrysalis protein enzymatic hydrolysis peptide
  • Method for decolorizing silkworm chrysalis protein enzymatic hydrolysis peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment one: the selection of decolorizing agent

[0013] 1% silkworm chrysalis proteolytic hydrolyzed antioxidant peptide solution, adjust the pH to 7, then add 20 g / L of powdered activated carbon and activated clay respectively, put it into the oscillating water bath, the temperature is 30 ° C, the speed is adjusted to 200 r / min, and after decolorization for 30 min, Centrifuge at 5000-6000r / min for 30 min in a disc centrifuge. Take the supernatant to measure its decolorization rate, nitrogen retention rate and reducing power retention rate. The results are shown in Table 1.

[0014] It can be seen from Table 1 that powdered activated carbon has a good decolorization effect, but at the same time of decolorization, the active polypeptide is also lost, which in turn affects the antioxidant activity of the supernatant. On the basis of a certain decolorization effect, activated clay better retains nitrogen content and reducing power.

[0015] Table 1 Decolorization e...

Embodiment 2

[0017] Embodiment two: the proportioning of composite decolorizing agent

[0018] 1% silkworm chrysalis protein enzymatic hydrolysis antioxidant peptide solution, adjust the pH to 7, add different proportions of powdered activated carbon and activated clay composite decolorizer 20 g / L, put it into an oscillating water bath, temperature 30°C, adjust the speed 200 r / min, decolorize After 30 min, centrifuge at 5000-6000 r / min for 30 min in a disc centrifuge. Take the supernatant to measure its decolorization rate, nitrogen retention rate and reducing power retention rate. The results are shown in Table 2.

[0019] It can be seen from Table 2 that with the decrease of the proportion of powdered activated carbon and the increase of the proportion of activated clay, the decolorization rate decreased, and the nitrogen retention rate and reducing power retention rate increased significantly. When the ratio of powdered activated carbon: activated clay is 1:7, the decolorization effec...

Embodiment 3

[0023] Embodiment three: the single factor optimization of the decolorization condition of compound decolorizer

[0024] 1% silkworm chrysalis protein enzymatic hydrolysis antioxidant peptide solution, adjust pH 5-9, add powdered activated carbon and activated clay compound decolorizer (1:7) 10-50 g / L, put it into an oscillating water bath, temperature 20-100 ℃, Adjust the rotation speed to 200 r / min, and after decolorization for 30 min, centrifuge at 5000-6000 r / min for 30 min in a disc centrifuge. Take the supernatant to measure its decolorization rate, nitrogen retention rate and reducing power retention rate. The results are shown in Table 3-5.

[0025] It can be seen from Table 3 that as the pH increases, the decolorization rate decreases, but the nitrogen retention rate and reducing power retention rate increase significantly.

[0026] It can be seen from Table 4 that the decolorization rate, nitrogen retention rate and reducing power retention rate do not change much ...

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Abstract

The invention relates to a method for decolorizing silkworm chrysalis protein enzymatic hydrolysis peptide. The method comprises the following steps of: adjusting pH of a 1 percent silkworm chrysalis protein enzymatic hydrolysis peptide solution to be 8, adding 30g of powdery activated carbon and activated clay composite decolorizer in a ratio of 1:7 into each liter of the solution, placing in an oscillatory type water bath kettle at the temperature of 30 DEG C, adjusting the rotation speed to be 200r/min, decolorizing for 30 minutes, and centrifuging by using a disk centrifuge at the speed of 5,000 to 6,000r/min for 30 minutes, wherein the decolorization rate is 70.39 percent, the nitrogen retention rate is 91.14 percent, and the reducing power retention rate is 86.79 percent. The process is simple and easy to operate, the treatment process is mild, and the method can be widely applied to health food industry in which silkworm chrysalis polypeptide and other bioactive peptides are produced.

Description

Technical field [0001] The invention involves a decolorization method of a silkworm pupa protease to resist oxidative peptide. Background technique [0002] Antioxidant peptide is a hotspot in recent years. Studies have found biological active peptides such as plant protein, animal protein, and microbial protein that can separate blood pressure peptides, immune regulatory peptides, antibacterial peptides, opioid peptides, and antioxidant peptides.Among them, antioxidant peptides can achieve antioxidant function by reducing various free radicals, inhibiting lipid peroxidation and chelating metal ions.Studies have been studied from proteases such as soybeans, corn, peanuts, and fish skin. [0003] my country is a large country nourishing, with an annual output of 650,000 tons of cocoon, accounting for 70%of the world's total cocoon.As a by -product of the silk industry, the processing rate of silkworm pupae is extremely low. Except for a few dried crushing as a nutritional additive...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
Inventor 廖鲜艳卢楠张义凯翁新楚黄滟
Owner SHANGHAI UNIV
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