Meaty reaction essence prepared by using enzymatic hydrolysate for larvae of barley pests
A technology for reacting flavors and barley worms, applied in application, food preparation, food science and other directions, can solve the problems of low protein content, high protein source prices, large market price fluctuations, etc., and achieve the effect of improving flavor intensity and low cost
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Embodiment 1
[0027] A preparation method of barley worm larva enzymatic hydrolyzate, comprising the steps of:
[0028] Put 350g of washed and crushed barley worm larvae and 150g of distilled water into the reactor, add a mixture of 0.3g of papain and 0.3g of neutral protease, stir for 10min, adjust the pH value to 6.5~7.0, heat up, and the reaction temperature Enzyme hydrolyze at 65°C for 1.5h, heat up to 100°C to inactivate enzyme for 15min after enzymolysis, cool down to 45°C after inactivation, let stand at room temperature for 20min, filter through 40-mesh sieve, filter out residue, collect filtrate to obtain barley Worm larvae hydrolyzate.
Embodiment 2
[0030] A preparation method of barley worm larva enzymatic hydrolyzate, comprising the steps of:
[0031] Wash and crush 400g of barley worm larvae, put 100g of distilled water in the reactor, add a mixture of 0.5g of trypsin and 0.3g of neutral protease, stir for 8min, adjust the pH value to 6.0~7.0, heat up, and the reaction temperature is Enzyme hydrolyze at 63°C for 1 hour, heat up to 98°C to inactivate the enzyme for 20 minutes after enzymolysis, cool down to 50°C after inactivating the enzyme, let stand at room temperature for 20 minutes, filter through a 40-mesh sieve, filter out the residue, and collect the filtrate to obtain barley worm larvae Enzyme solution.
Embodiment 3
[0033] A method for preparing a beef reaction essence obtained by applying the enzymatic hydrolysis solution of barley worm larvae, comprising the following steps:
[0034] Get beef enzymolysis solution: 45g, barley worm larva enzymolysis solution prepared in Example 1: 40g, refined butter: 5g, cysteine: 0.5g, glutamic acid: 0.5g, proline: 0.5g , Methionine: 0.5g, Glucose: 4.5g, VB 1 : 0.3g, I+G: 0.5g, HVP: 1g, yeast extract: 0.5g, ginger oil 0.2g, garlic oil 0.5g, scallion mud 0.5g join the reactor, stir 10min after fully mixing, Heating to raise the temperature, controlling the Maillard reaction temperature at 100°C for 1 hour, then lowering it to 45°C after the reaction, leaving it at room temperature for 25 minutes and filtering it with a 40-mesh sieve, the filtrate is the beef reaction flavor.
[0035] Wherein the preparation method of beef enzymatic hydrolyzate:
[0036] Put 400g of washed and minced beef and 100g of distilled water into the reactor, add 0.5g of papain...
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