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Bacillus sp. UTM03 and application thereof

A technology of UTM03 and Bacillus, applied in the field of environmental microorganisms, can solve the problems of increasing the workload and cost of sludge composting, lack of effective thermophilic microbial populations, and ignoring excellent conditioners, so as to reduce the cost of composting, improve economic benefits and social Benefit, excellent performance

Active Publication Date: 2012-10-03
海南绿源科创环境技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, Bacillus subtilis, Bacillus licheniformis, etc. are the main strains used in composting, but these strains are mesophilic bacteria. After promoting composting into a high-temperature period, they will lose their activity because they cannot tolerate high-temperature environments.
For example, the screening temperature of the high-temperature microbial strains Geobacillus and Bacillus licheniformis used in the patent (application number: 200910035641.X) is 50°C, which cannot guarantee that they will still be active in the high-temperature period of sludge composting above 70°C
Therefore, the compost products obtained by such conventional compost inoculants lack sufficient effective thermophilic microbial populations, and the inoculum needs to be re-prepared every time composting, which greatly increases the workload and cost of sludge composting, and also ignores the The Function of Return Material as an Excellent Conditioner for Wet Sludge

Method used

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  • Bacillus sp. UTM03 and application thereof
  • Bacillus sp. UTM03 and application thereof
  • Bacillus sp. UTM03 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Identification of Bacillus UTM03

[0029] In a sterile environment, weigh 5g of the sludge compost sample into a 50ml sterile water triangular flask equipped with 10 glass beads, place it on a shaker and vibrate at 160r / min for 30 minutes to fully disperse the sample, and then place it for 70 ℃, static culture for 12 hours.

[0030] Take out the above-mentioned triangular flask for static culture, and let it stand for 3 minutes in a sterile environment, then use a sterile straw to draw 5ml of supernatant and add it to 50ml of sterilized bacterial culture solution, shake well, and place At 70° C., static enrichment culture was carried out for 48 hours, and thus subcultured for 4 generations. The formula of the bacterial liquid medium is: 0.5 g of beef extract, 1 g of peptone, 0.5 g of sodium chloride, 100 ml of distilled water, pH 7.2-7.4, and sterilized at 121° C. for 20 minutes.

[0031] Take out the enrichment culture flask of the above 4 gen...

Embodiment 2

[0035] Example 2 Preparation of Fermented Seed Liquid

[0036] Take the slant of the bacterial strain Bacillus sp.UTM03 of the present invention and activate and cultivate it at 70°C for 8 hours, then inoculate it into a shaker flask filled with sterilized LB culture solution, place it on a water bath constant temperature oscillator for primary expansion, and the oscillation frequency 160rpm, time 36 hours, temperature 70°C, culture solution OD was tested 600 It is 2.523; take the primary culture liquid and carry out expansion cultivation in the fermenter, control the temperature at 65 ° C, stir for 15 minutes at intervals of 6 hours, rotate at 150 rpm, ventilate for 15 minutes at intervals of 6 hours, and the ventilation rate is 1: 1.2 (V / V). After 3 days, detect the OD of the fermentation broth 600 It is 3.003, which is the fermented seed liquid.

Embodiment 3

[0037] Example 3 Preparation of Fermented Seed Liquid

[0038] Take the culture slant of the bacterial strain Bacillus sp.UTM03 of the present invention and activate it at 65°C for 12 hours, then inoculate it into a shaker flask filled with sterilized LB culture solution, place it on a water-bath constant temperature oscillator for primary expansion, and the oscillation frequency 180rpm, time 36 hours, temperature 65°C, the OD600 of the culture medium was detected to be 2.815; take the first-grade culture medium and expand it in the fermenter, control the temperature at 65°C, stir for 10 minutes at intervals of 4 hours, and stir at 160rpm at intervals of 4 hours Ventilate for 10 minutes, and the ventilation rate is 1:0.8 (V / V). After 4 days of fermentation, the OD600 of the fermented liquid is detected to be 3.103, which is the fermented seed liquid.

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Abstract

The invention provides a Bacillus sp. strain UTM03. The preservation number of the Bacillus sp. strain UTM03 is CGMCC No.5643. The strain UTM03 can be quickly proliferated at the temperature of 70 DEG C. The invention also provides application of the strain UTM03 in sludge composting. According to a seed compost prepared by using the strain UTM03 and a sludge composting application method for thestrain UTM03, the problem that microorganisms are inactivated or a compost product does harm to plants or heavy metals exceed the standard due to the concentration of salt or heavy metals in the sludge composting can be solved; and the strain can be widely applied to the sludge composting, and has good economical and social benefits.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a Bacillus strain UTM03 and its application. Background technique [0002] Sludge is solid waste produced by sewage treatment plants. With the continuous increase in the number of existing sewage treatment plants and the continuous innovation of treatment processes, the volume and degree of sewage treatment have been continuously improved, and the sludge output has increased significantly. At present, the methods of sludge disposal in most sewage treatment plants mainly include: incineration, landfill, throwing into the sea and composting. Relatively speaking, sludge high-temperature composting is a process in which organic matter is converted into stable humus through the biological metabolism of aerobic microorganisms, releasing heat, and its treatment cost is low, and it can effectively kill pathogenic bacteria, parasite eggs and weed seeds , and can eva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/00C12R1/07
CPCY02W30/40
Inventor 刘永跃周顺桂许宜北汪涌
Owner 海南绿源科创环境技术有限公司
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