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Electrochemical immunosensor for directly detecting IL-6 (interleukin-6) antigen and application thereof

An immunosensor, the technology of IL-6, is applied in the field of electrochemical immunosensor and preparation for directly detecting IL-6, and achieves the effects of short assembly time, simple preparation process and high detection sensitivity

Inactive Publication Date: 2012-10-03
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is rare to directly electrodeposit nano-gold particles on carbon nanotubes grown in situ to construct electrochemical immunosensors.

Method used

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  • Electrochemical immunosensor for directly detecting IL-6 (interleukin-6) antigen and application thereof
  • Electrochemical immunosensor for directly detecting IL-6 (interleukin-6) antigen and application thereof
  • Electrochemical immunosensor for directly detecting IL-6 (interleukin-6) antigen and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Preparation of SWCNTs-Au hybrid electrode

[0034] For the preparation of Fe / Mo nanoparticles, refer to Chem. Mater. 2001, 13, 1008-1014. The ratio of Fe / Mo substances used in the Fe / Mo nanoparticles used in the present invention is 5:1.

[0035] Such as figure 1 As shown, the chemical vapor deposition (CVD) method is used. Firstly, a 1×1 cm silicon substrate is immersed in a heptane solution of Fe / Mo nanoparticles (the amount of Fe / Mo nanoparticles is based on the amount of Fe substance, 5 mmol / L), 10s Then take it out, dry it in the air, transfer it to a quartz tube (length 80cm, outer diameter 25mm, thickness 1.5mm), then use a heating furnace to heat up the temperature of the quartz tube to 1000°C, use ethanol as the carbon source, and use hydrogen at a flow rate of 200ml / min as the Carrier gas and protective gas of carbon source, bring ethanol vapor (carbon source) into the quartz tube, react at 1000°C for 15 minutes, and obtain single-walled carbon nanotubes...

Embodiment 2

[0044] Put the electrochemical sensor obtained by the method of Example 1 into a concentration of 1 × 10 -16 In the PBS buffer solution (pH=7.2) of the IL-6 antigen of g / ml, immunocombination was performed at 37° C. for 30 min, other operations were the same as in Example 1, and the electrochemical impedance curve was recorded (such as image 3 Middle curve f), which is obviously different from the AC impedance curve obtained by the working electrode not combined with IL-6 antigen (such as image 3 Middle curve d), calculate its transfer resistance change value as 25.7kΩ (take the transfer resistance change value as the ordinate, and take the logarithm of the antigen concentration as the abscissa to make a standard curve, such as Figure 5 shown).

Embodiment 3

[0046] Put the electrochemical sensor obtained by the method of Example 1 into a concentration of 1 × 10 -15 In the PBS solution of the IL-6 antigen of g / ml, at 37 ℃, immunocombination 30min, other operation is the same as embodiment 1, records the electrochemical impedance curve (such as image 3 Middle curve g), which is obviously different from the AC impedance curve obtained by the working electrode not combined with IL-6 antigen (such as image 3 Middle curve d), calculate its transfer resistance change value as 28.9kΩ (take the change value of transfer resistance as the ordinate, and take the logarithm of the antigen concentration as the abscissa to make a standard curve, such as Figure 5 shown).

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Abstract

The invention discloses an electrochemical immunosensor for directly detecting IL-6 (interleukin-6) antigen and application thereof. The immunosensor uses a single-walled carbon nanotube as a base. Gold nanoparticles are sedimentated on the single-walled carbon nanotube to form a single-walled carbon nanotube / gold nanoparticle hybrid electrode. The hybrid electrode is modified by thioglycolic acid and IL-6 is assembled on the thioglycolic acid to capture antibodies. The antibodies are then sealed by PBS (phosphate buffer solution) of bovine serum albumin in mass concentration of 1-5% to obtain the electrochemical immunosensor. The electrochemical immunosensor has the advantages that preparation process is simple and convenient, no marking or sandwich treatment is needed, cost is low, detecting sensitivity is high and the like. The electrochemical immunosensor is widely applicable to various immunoassays and detections. In addition, detection limit of the electrochemical immunosensor to the target IL-6 antigen can reach 1*10-17g / ml.

Description

(1) Technical field [0001] The invention relates to an immunosensor, in particular to an electrochemical immunosensor for directly detecting IL-6, a preparation method and a detection application. (2) Background technology [0002] Interleukin 6 (IL-6), which can be produced by lymphocytes or non-lymphocytes, is a multifunctional cytokine that not only plays an important role in the body's immune response, acute phase response, and hematopoietic control, but also its dysregulated production is related to various On-the-spot diseases and inflammatory responses are closely related, such as psoriasis, rheumatoid arthritis, cardiovascular disease and inflammatory bowel disease. In addition, its overexpression is associated with several typical cancers, such as head and neck squamous cell carcinoma (HNSCC), colon cancer, and gastrointestinal cancer. However, the concentration of interleukin-6 in normal human body (6pg / ml) is very low, therefore, the detection of low-concentratio...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 王舜陈锡安金辉乐杨婷
Owner WENZHOU UNIVERSITY
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