Duck virus hepatitis yolk antibody freeze-dried powder and preparation method thereof

A technology for duck viral hepatitis and egg yolk antibody, applied in the direction of antibodies, freeze-dried delivery, antiviral agents, etc., can solve the problems of labor-intensive and time-consuming, and achieve the effect of wide immunity, remarkable curative effect and quick effect.

Inactive Publication Date: 2012-10-10
ZHENGZHOU HOUYI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in actual production, animal blood collection is not only a great stress on the animal itself, but also time-consuming and labor-intensive. Large-scale production is impractical, and it is much more convenient to use laying hens to produce antibodies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1. The freeze-dried powder of duck viral hepatitis egg yolk antibody in this example includes the following components: duck viral hepatitis egg yolk antibody 85.99%, caprylic acid 0.8%, thimerosal 0.01%, formaldehyde 0.2%, glucose 5%, sorbitol 2 %, glycine 3%, mannitol 3%.

[0023] The preparation method of the present embodiment duck viral hepatitis hyperimmune yolk antibody freeze-dried powder, concrete steps are:

[0024] 1) Immunization with duck viral hepatitis inactivated vaccine 1.5mL (containing 10 32 ELD 50 / 0.1Ml), the healthy duck population was immunized with duck virus hepatitis inactivated vaccine for the first time, and the interval between each immunization was 5 days, and the amount of subsequent immunizations was doubled, and immunized three times, and the antibody titer was detected after the second immunization. When the antibody titer reaches 1:256, start collecting eggs, and the collected eggs are cleaned, disinfected, and dried for l...

Embodiment 2

[0049] Example 2. The freeze-dried powder of duck viral hepatitis egg yolk antibody in this example includes the following components: duck viral hepatitis egg yolk antibody 87.735%, caprylic acid 1.0%, thimerosal 0.015%, formaldehyde 0.25%, glucose 4.5%, sorbitol 1.5% %, glycine 2.5%, mannitol 2.5%.

[0050] The preparation method of the present embodiment duck viral hepatitis hyperimmune yolk antibody freeze-dried powder, concrete steps are:

[0051] 1) Immunization with duck viral hepatitis inactivated vaccine 1.5mL (containing 10 3.2 ELD 50 / 0.1Ml), the healthy duck population was immunized with duck virus hepatitis inactivated vaccine for the first time, and the interval between each immunization was 5 days, and the amount of subsequent immunizations was doubled, and immunized three times, and the antibody titer was detected after the second immunization. When the antibody titer reaches 1:256, start collecting eggs, and the collected eggs are cleaned, disinfected, and d...

Embodiment 3

[0076] Embodiment 3. The freeze-dried powder of duck viral hepatitis egg yolk antibody in this embodiment includes the following components: duck viral hepatitis egg yolk antibody 86.68%, thimerosal 0.02%, formaldehyde 0.3%, 5% glucose, 2% sorbitol, 3% Glycine, 3% Mannitol.

[0077] The lyophilized powder of duck viral hepatitis hyperimmune egg yolk antibody in this embodiment can be prepared by a conventional lyophilized powder preparation method.

[0078] In order to detect the change of antibody titer when the egg yolk antibody is placed at room temperature, so as to determine the shelf life of this product, the following tests were done: the product was stored in a 37°C incubator for 24 months, respectively for 1 week and 2 days. Weeks, January, February, April, August, December, 16 months, 20 months, and 24 months later, use the agar diffusion (AGP) method to measure the changes in the yolk antibody titer of samples taken at each time point, and the results See Table 3. ...

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Abstract

The invention relates to a duck virus hepatitis yolk antibody freeze-dried powder which comprises the following components: 85-88% of duck virus hepatitis yolk antibody, 0.8-1.0% of octanoic acid, 0.01-0.02% of thimerosal, 0.2-0.3% of formaldehyde, 4-5% of glucose, 1-2% of sorbic alcohol, 2-3% of glycine and 2-3% of mannitol. The freeze-dried powder has the advantages of long preservation time, high dissolving speed, high antibody titer and excellent biological activity, and more importantly, the purity of the antibody of the product is higher than that of the existing product on the market. At the same time, the invention also discloses a preparation method of the duck virus hepatitis yolk antibody freeze-dried powder, comprising the following steps: voluntarily separating strains in a disease prevalence area to prepare a vaccine, immunizing healthy dusks to obtain hyper-immune eggs, ad performing disinfection, acidification, octanoic acid treatment, refining extraction and purification, preparation, freezing and the like on the hyper-immune eggs to obtain the duck virus hepatitis yolk antibody freeze-dried powder. According to the preparation method in the application, a yolk solution is heated at the temperature of pasteurization when the acidification water treatment is carried out, so that not only is the disinfection purpose fulfilled, but also the recovery rate of the antibody and the clarity of the solution in the acidification treatment are increased, the prepared yolk antibody has the advantages of high biological activity, long antibody preservation time, titer reduction, lowered speed and the like.

Description

technical field [0001] The invention belongs to the technical field of biological products for poultry, in particular to a freeze-dried powder of egg yolk antibody against duck viral hepatitis and a preparation method thereof. Background technique [0002] Duck viral hepatitis, referred to as duck hepatitis, is one of the most serious diseases that harm ducklings. It mainly harms ducklings less than 20 days old. The younger the ducklings, the higher the mortality rate, and the highest mortality rate can reach 90%-95. %, and all antibiotics are ineffective against this disease. The disease was discovered for the first time in the United States in 1945 and named as type I duck viral hepatitis. In 1965, duck viral hepatitis type II was discovered in the United Kingdom. In 1969, duck viral hepatitis type III was discovered in the United States. Duck viral hepatitis virus type I belongs to the picornaviridae family. It is spherical or quasi-spherical, with a diameter of 20-40NM....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61K9/19A61P31/14
Inventor 吴红云李建正郭俊清徐进
Owner ZHENGZHOU HOUYI PHARMA
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