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High-activity blood pressure lowering peptide and preparation method thereof

A blood pressure lowering peptide and high activity technology, applied in the field of high activity blood pressure lowering peptide and its preparation, can solve the problem that natural blood pressure lowering peptide needs to be improved and the like

Active Publication Date: 2012-10-31
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current methods for preparing natural hypotensive peptides still need to be improved

Method used

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  • High-activity blood pressure lowering peptide and preparation method thereof
  • High-activity blood pressure lowering peptide and preparation method thereof
  • High-activity blood pressure lowering peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] First, dissolve defatted walnut powder and water at a ratio of 1:10, hydrolyze for 5 hours at pH 8.0, hydrolysis temperature 65°C, stop the hydrolysis reaction, centrifuge to take the supernatant, and carry out acid precipitation under the condition of isoelectric point 4.5 , centrifuged to take the precipitate, washed the precipitate to neutrality, and then it was quickly vacuum freeze-dried and stored, and its protein content was determined to be 86.32% by the Coomassie brilliant blue method.

[0052] Then, use protease to carry out hydrolysis respectively, hydrolysis conditions:

[0053] 1) Papain: The ratio of solid to liquid is 25g / L, the amount of enzyme added is 3% (m / v), the pH is 6, the temperature is 50°C, and the hydrolysis time is 4h.

[0054] 2) Pepsin: The ratio of solid to liquid is 25g / L, the amount of enzyme added is 4% (m / v), pH 2, the temperature is 55°C, and the hydrolysis time is 4h.

[0055] 3) Alkaline protease: The ratio of solid to liquid is 25...

Embodiment 2

[0060] The lyophilized powder of pepsin and alkaline protease with high ACE inhibitory activity and molecular weight less than 5000 obtained in Example 1 was dissolved in 5ml of 10mM Tris-HCl (pH 7.0), and degassed and passed through the membrane , separated and purified by Sephadex G25 Medium column (i.e. the first gel chromatography treatment), the elution peak was detected at 280nm and collected to determine the ACE inhibitory activity of each component (the detection method of ACE inhibitory activity is as described in the general method), wherein Chromatogram see figure 1 , the activity test results are shown in Table 2 below, and the collected solution was quickly vacuum freeze-dried.

[0061] Table 2 The IC of the ACE inhibitory activity of the different components obtained from the first gel chromatography 50

[0062]

[0063] figure 1 Shows the chromatograms of the first gel chromatographic treatment during the preparation of highly active antihypertensive pepti...

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Abstract

The invention relates to high-activity blood pressure lowering peptide and a preparation method thereof, wherein the high-activity blood pressure lowering peptide has an amino acid sequence shown by SEQ ID NO: 1. The high-activity blood pressure lowering peptide has the advantages of low cost, high angiotensin-converting enzyme inhibition activity and capability of effectively inhibiting the activity of angiotensin-converting enzyme, and can be therefore effectively applied to preparation of medicaments for treating hypertension.

Description

technical field [0001] The invention relates to the field of health-care functional food and medicine preparation. Specifically, the present invention relates to highly active hypotensive peptide and its preparation method. Background technique [0002] Hypertension is a common cardiovascular and cerebrovascular disease. It has a high incidence rate and is often accompanied by changes in the heart, blood vessels, lungs, and kidneys. It can cause stroke, coronary heart disease and other diseases. At present, drugs containing artificially synthesized antihypertensive peptides are mostly used in the clinical treatment of hypertension, but it has been found that such antihypertensive peptides may produce side effects to varying degrees during clinical application. Therefore, it is of great significance to search for natural and safer antihypertensive peptides. [0003] However, the current methods for preparing natural hypotensive peptides still need to be improved. Contents...

Claims

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Application Information

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IPC IPC(8): C07K5/087C12P21/06C07K1/36C07K1/34C07K1/20C07K1/16A61P9/12
Inventor 王建中顾欣王丰俊侯雅坤
Owner BEIJING FORESTRY UNIVERSITY
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