Method for extracting hematopoietic stem cell from placental chorionic tissue and construction method of hematopoietic stem cell

Abstract

The invention discloses a method for extracting a CD133 positive stem cell from a placental chorionic tissue. The method comprises the following steps: (1) collecting a placenta to a placenta collection box, and adding protection fluid into the collection box; (2) carrying out information registration and serological virus detection before the placenta separates the stem cell; (3) washing blood clot and hematocele in the placenta tissue; (4) separating the placenta stem cell by a mechanical method; (5) concentrating and purifying the placenta stem cell, and extracting the CD133 positive stem cell from the placenta; and (6)storing the CD133 positive stem cell. The stored CD133 positive stem cell can be used for the transplanting of a hematopoietic stem cell, angiogenesis, repairing of nerve injury and the like. The invention has the advantages that the CD133 positive stem cell extracting flow is convenient; theCD133 positive stem cell extracting cost is lower; and a whole placenta can be treated rapidly at a time, the placenta contains a large number of mononuclear cells, and the content of CD133 positive stem cells in the placenta reaches 2.0 to 5.0 percent. The technology is also applicable to constructing a CD133 positive stem cell bank, and the CD133 positive stem cells stored in the cell bank can be reused by adults for many times and stored for a long time, and are convenient to use at any time.

Description

technical field [0001] The invention relates to a method for extracting hematopoietic stem cells from placental chorionic tissue, in particular to a method for extracting CD133 positive stem cells from placental chorionic tissue, and a method for using the extracted CD133 positive stem cells to construct a hematopoietic stem cell bank. Background technique [0002] The stem of a hematopoietic stem cell (HSC), translated from English "stem", means "tree", "stem" and "origin". Similar to a tree trunk can grow branches, leaves, and blossom and bear fruit. In layman's terms, hematopoietic stem cells refer to immature cells, which are the origin of all hematopoietic cells and immune cells. They can not only differentiate into red blood cells, white blood cells and platelets, but also differentiate into cells of various tissues and organs across systems. Renewal, multilineage differentiation, and homing (ie, directed migration to hematopoietic tissues and organs) potential. Gene...

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Problems solved by technology

[0007] At present, the methods for isolating placental stem cells include mechanical separation, lavage, and collagenase digestion. The mechanical separation causes great damage to the cells and is time-consuming and laborious for separation, which is not conducive to industrialization. The lavage method is mainly used for hemangioblasts, and the cells are obtained. The rate is low; collagenase digestion method Neizhou victory, etc.

[0008] The new method of extracting hematopoietic stem cells from placental tissue for the establishment of hematopoietic stem cell bank (Patent No. 01131190.8) mentions that CD34-positive cells from tissue are mainly sorted, and a single collagenase is used to digest placental tissue for 15 minutes, but The above method can only obtain a very small number of cells, and the number of cells is far lower than that obtained by mechanical separation.

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