Porcine Asian type-I foot-and-mouth disease virus inactivated vaccine adjuvant and preparation method thereof

A technology for foot-and-mouth disease virus and inactivated vaccine, which is applied in the field of vaccine adjuvants, can solve the problems of inconvenience, reduced activity, short half-life, etc. of cytokine recombinant protein, and achieves the effects of good cell growth state, low cost, and low toxicity and side effects.

Active Publication Date: 2012-11-07
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of recombinant protein as a vaccine adjuvant also has the following disadvantages: the half-life is short, the activity is easily affected by the internal and external environment and the activity is reduced, and multiple injections are required; in addition, the one-time use of large doses can also cause side effects such as fever and inflammation. This brings inconvenience to the clinical application of cytokine recombinant protein

Method used

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  • Porcine Asian type-I foot-and-mouth disease virus inactivated vaccine adjuvant and preparation method thereof
  • Porcine Asian type-I foot-and-mouth disease virus inactivated vaccine adjuvant and preparation method thereof
  • Porcine Asian type-I foot-and-mouth disease virus inactivated vaccine adjuvant and preparation method thereof

Examples

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example 1

[0021] Example 1: Preparation and Characterization of Recombinant Porcine Interleukin 2 Solid Lipid Nanoparticles

[0022] 1.1 Preparation, characteristics and characterization of solid lipid nanoparticles

[0023] The preparation process by adopting the double emulsion solvent evaporation method is as follows: Weigh 100 mg of hydrogenated castor oil and 20 mg of PLGA dissolved in 1 mL of chloroform as the organic phase, and heat the organic phase to 30-40 ° C; the purified IL- 2 Dissolve the protein in PBS (pH=8), take 200 μL as the water phase; then slowly add the water phase to the organic phase, stir with a magnetic stirrer while adding, ultrasonicate in an ice bath for 50s (300w) and form (w / o) form of emulsion; the prepared emulsion was added to 5mL of 1% polyvinyl alcohol (PVA) aqueous solution, and further emulsified by emulsifier milk (rpm3000, 1min) to form (w / o / w) Phase emulsion, and observe under a microscope to emulsify into uniformly distributed droplets; then ...

example 2

[0033] Example two: The prepared nanoparticle is used as the adjuvant of Asian type I foot-and-mouth disease virus inactivated antigen to form the vaccine immune effect test

[0034] 2.1 Materials and methods

[0035] 2.1.1 Preparation of the vaccine

[0036] The tested vaccines in the present invention were prepared from the previous examples containing recombinant pIL-2 nanoparticles (SLN-pIL-2), empty SLN, recombinant pIL-2, and commercially purchased complete Freund's adjuvant (FCA) as different vaccines. Adjuvant and Asian I type FMDV virus inactivated antigen (hereinafter referred to as Ag in the test, provided by Nongweit Biotechnology Co., Ltd.) shock and mix to prepare vaccines containing different adjuvants, the volume ratio of the added adjuvant to antigen 1:1.

[0037] 2.1.2 Experimental animals and immunization

[0038] Sixty female BALB / c mice aged 6-8 weeks were purchased from the Experimental Animal Center of Lanzhou Institute of Biological Products and test...

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Abstract

The invention relates to a porcine Asian type-I foot-and-mouth disease virus inactivated vaccine adjuvant and a preparation method thereof. The adjuvant comprises recombined porcine interleukine-2 (pIL-2). The porcine Asian type-I foot-and-mouth disease virus inactivated vaccine adjuvant is a nanoparticle with the particle size of 50-1000nm, wherein the nanoparticle is formed by wrapping the porcine interleukine-2 with a lipid matrix and an emulsifier.

Description

technical field [0001] The invention relates to an adjuvant for vaccines, in particular to an adjuvant for veterinary vaccines. Specifically, the present invention relates to an adjuvant and a preparation method of an inactivated vaccine of Asia-I foot-and-mouth disease virus for pigs. The adjuvant contains recombinant porcine interleukin-2 (Porcine Interleukine-2, pIL-2). Background technique [0002] An adjuvant is a substance that is first mixed with an antigen or injected into the human or animal body at the same time, which can enhance the body's specific immune response to the antigen and play an auxiliary role. Adjuvants are the main components of vaccines and play an important role in improving and improving the quality of vaccines. [0003] Interleukin-2 (IL-2) is a cytokine with multiple functions, which can act on the surface of some cells in an autocrine or paracrine manner, thereby initiating the signaling pathway of cells in the body and playing an immune regu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/135A61P31/14
Inventor 景志忠陈国华曾爽房永祥贾怀杰何小兵蔺国珍才学鹏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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