Method for extracting proteins from vitamin C fermented mash

A technology for fermenting mash and protein, applied in the biological field, can solve problems such as environmental pollution, and achieve the effects of environmental protection, simple equipment, and rich varieties

Active Publication Date: 2012-11-07
SHANDONG TIANLI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a method for extracting protein from vitamin C fermentation by-product—vitamin C fermented mash. The method can extract protein from mash conveniently and quickly, Realize the comprehensive utilization of resources, that is, solve the problem of environmental pollution caused by fermentation mash, and turn waste into treasure to reduce the waste of resources, which has very good economic and sociological significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Ultrafiltration treatment of fermented mash

[0029] Take 100g of vitamin C fermentation mash and process it through an ultrafiltration membrane system with a molecular weight cut-off of 2000 Daltons. After treatment, by-products such as mycelium and protein were intercepted, and the interception rate reached 98.0%, and the intercepted components were collected.

[0030] (2) Crushing treatment

[0031] Collect the retained components, add distilled water and stir evenly, the volume ratio of water to retained components is 1:0.5, then put the mixed liquid into the ultra-low temperature quick-freezing equipment, freeze at -35°C for 5 minutes, and then freeze under the conditions of pressure 120Mpa and flow rate 200m / s The suspension was crushed by a high-pressure homogenizer, and the cell crushing rate was 94%.

[0032] (3) Enzymatic hydrolysis

[0033] Add 0.35% protein complex enzyme (carboxypeptidase, alkaline protease, papain mass ratio: 1:1:1) to the...

Embodiment 2

[0041] (1) Ultrafiltration treatment of fermented mash

[0042] Take 100g of vitamin C fermented mash and process it through an ultrafiltration membrane system with a cut-off of 1500 Daltons. By-products such as body and protein are intercepted, and the retention rate reaches 97.5%, and the retained components are collected.

[0043] (2) Crushing treatment

[0044]Collect the retained components, add distilled water and stir evenly, the volume ratio of water to retained components is 1:1, then put the mixed liquid into the ultra-low temperature quick-freezing equipment, freeze at -50°C for 8 minutes, and then freeze under the conditions of pressure 100Mpa and flow rate 260m / s The suspension was crushed by a high-pressure homogenizer, and the cell crushing rate was 95.5%.

[0045] (3) Enzymatic hydrolysis

[0046] Add 1.2% protein complex enzyme (carboxypeptidase, papain mass ratio: 1:1) to the crushed slurry to enzymatically hydrolyze the protein. The reaction te...

Embodiment 3

[0054] (1) Ultrafiltration treatment of fermented mash

[0055] Take 100g of vitamin C fermentation mash and process it through an ultrafiltration membrane system with a cut-off of 1000 Daltons, control the inlet water pressure at 0.2-0.25Mpa, adjust the pH value of the fermentation mash to 6.0, and operate at a temperature of 29-33°C. By-products such as mycelium and protein are retained, with a retention rate of 99.0%, and the retained components are collected.

[0056] (2) Crushing treatment

[0057] Collect the retained components, add distilled water and stir evenly, the volume ratio of water to retained components is 1:1.5, then add the mixed liquid to the ultra-low temperature quick-freezing equipment, freeze at -50°C for 10 minutes, and then freeze under the conditions of pressure 150Mpa and flow rate 150m / s The suspension was crushed by a high-pressure homogenizer, and the bacterial cell crushing rate was 99.0%.

[0058] (3) Enzymatic hydrolysis

[0059] ...

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Abstract

The invention discloses a method for extracting proteins from vitamin C fermented mash, which comprises the following steps: ultrafiltering the vitamin C fermented mash via an ultrafiltering membrane, and collecting the intercepted components; adding water into the intercepted components so as to uniformly mix, quickly freezing the intercepted component solution via an ultralow temperature quick-freezing technology, and then crushing the quick-frozen intercepted component solution via a high-pressure homogenizer; adding a compound protease into the crushed suspension, extracting the protein components via enzymolysis, centrifugally separating enzymatically decomposed solution obtained by enzymolysis, and collecting liquid supernatant; adjusting the pH value of the liquid supernatant, and carrying out flocculating settling for the proteins; and drying the precipitate to obtain the albumen powder. According to the invention, the albumen powder is extracted by a vitamin C fermented by-product, a wide variety of amino acid in the extracted proteins is capable of being taken as feed proteins and raw materials for deeply processing the proteins, thereby realizing comprehensively and effectively utilizing the vitamin C fermented by-product, increasing a new channel of the resource of the feeding albumen powder, and saving the resources and protecting the environment.

Description

technical field [0001] The invention relates to a method for extracting protein from vitamin C fermented mash, in particular to a production technology for collecting and crushing mycelium and separating and extracting protein, belonging to the field of biotechnology. Background technique [0002] Vitamin C is an indispensable substance for maintaining human health. It is a catalyst for the oxidation-reduction reaction of living cells, participates in various metabolisms in the body, and has the physiological effect of promoting various rapid synthesis in the body. The lack of vitamin C in the body is prone to scurvy, which can cause death in severe cases. Clinical application: prevent and treat scurvy; promote body metabolism, enhance anti-infection ability, prevent the production of carcinogens; first aid for various diseases; make tissue produce collagen to promote wound healing; help enzymes convert cholesterol into cholesterol and excrete it , to reduce the fragility ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 王乔隆付吉明张全景郑秀宁刘敏庄祎
Owner SHANDONG TIANLI PHARMA
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