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Preparation method and application of mulberry leaf phenolic acid extract with antibacterial activity

A technology of mulberry leaf phenolic acid and antibacterial activity, applied in the field of mulberry leaf extract, can solve the problems of large loss of functional active components, low content of functional active components, destruction and the like, achieve sufficient extraction and elution, and simple preparation process , the effect of reducing the amount of addition

Inactive Publication Date: 2012-11-14
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above methods, the processing of plant beverages is mainly completed by high-temperature extraction and high-temperature concentration in neutral water. The active antibacterial components of plants are not only insufficiently extracted, but also severely damaged after a long period of high temperature. The loss of active ingredients is relatively large, and the content of functional active ingredients in plant beverages made by this conventional process is low, and the antibacterial active ingredients are less, and the efficacy is poor, so it is difficult to really achieve the effect of clearing away heat and relieving summer heat

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  • Preparation method and application of mulberry leaf phenolic acid extract with antibacterial activity
  • Preparation method and application of mulberry leaf phenolic acid extract with antibacterial activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of mulberry phenolic acid extract extract:

[0039] (1) Extraction: Mix dry mulberry leaf powder and 50% acidic ethanol solution with pH 2.0 at a ratio of 1:10 (W / V), sonicate for 0.5 min, then extract at a constant temperature of 45 °C for 6 hours, separate the extract, and repeat The above operations were extracted 3 times, the extracts were combined, filtered, and concentrated under reduced pressure at 40°C until ethanol-free to obtain the crude extract of mulberry phenolic acid.

[0040] (2) Adsorption: adjust the pH value of the crude extract of mulberry leaf phenolic acid to 2.0~4.0, and the concentration is 3.0 mg / mL, perform HPD-750 macroporous adsorption resin column chromatography, add mulberry leaves in the macroporous adsorption resin column The volume of the phenolic acid crude extract was 5.0 column volumes, and the adsorption flow rate was 3.5 column volumes / h.

[0041] (3) Elution: After adsorption equilibrium, use 50% acidic ethanol solutio...

Embodiment 2

[0046] Preparation of mulberry phenolic acid extract extract:

[0047] (1) Extraction: Take dry mulberry leaf powder and 60% acidic ethanol solution with a pH of 3.0 and mix them at a ratio of 1:15 (W / V), treat with ultrasound for 0.8min, then extract at a constant temperature of 45°C for 5 hours, separate the extract, and repeat The above operations were extracted 3 times, the extracts were combined, filtered, concentrated under reduced pressure at 45°C until ethanol-free, and the crude extract of mulberry phenolic acid was obtained.

[0048] (2) Adsorption: adjust the pH value of the crude extract of mulberry phenolic acid to 2.0~4.0, and the concentration is 4.0 mg / mL, perform HPD-750 macroporous adsorption resin column chromatography, add the mulberry phenol in the porous adsorption resin column The volume of the crude acid extract was 6.0 column volumes, and the adsorption flow rate was 4.5 column volumes / h.

[0049] (3) Elution: After adsorption equilibrium, use 60% aci...

Embodiment 3

[0054] Preparation of mulberry phenolic acid extract extract:

[0055] (1) Extraction: Mix dry mulberry leaf powder and 70% acidic ethanol solution with pH 4.0 at 1:20 (W / V), ultrasonicate for 1.0 min, then extract at a constant temperature of 45°C for 4 hours, separate the extract, and repeat The above operations were extracted twice, the extracts were combined, filtered, concentrated under reduced pressure at 50°C until ethanol-free, and the crude extract of mulberry phenolic acid was obtained.

[0056] (2) Adsorption: adjust the pH value of the crude extract of mulberry phenolic acid to 2.0~4.0, the concentration is 5.0 mg / mL, perform HPD-750 macroporous adsorption resin column chromatography, add the mulberry phenol in the porous adsorption resin column The volume of the acid crude extract was 7.0 column volumes, and the adsorption flow rate was 5.5 column volumes / h.

[0057] (3) Elution: After adsorption equilibrium, use 70% acidic ethanol solution with a pH of 2.0~-4.0 ...

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Abstract

The invention discloses mulberry leaf phenolic acid extract with antibacterial activity. A preparation method of the mulberry leaf phenolic acid extract includes steps of firstly, mixing dry mulberry leaf powder with 50-70% acidic ethanol solution according to a proportion of 1:10-1:20, performing ultrasonic treatment for 0.5-1 minute, extracting at a constant temperature of 45 DEG C for 4-6 hours, separating extract, repeating above operations for extraction for 2-3 times, combing extract, filtering, and allowing for concentration under reduced pressure at 40-50 DEG C until no ethanol exists to obtain mulberry leaf phenolic acid crude extract; secondly, adjusting Ph value of the mulberry leaf phenolic acid crude extract under the concentration of 3.0-5.0mg / mL to 2.0-4.0 and performing macroporous absorption resin column chromatography; thirdly using the 50-70% acidic ethanol solution with Ph value of 2.0-4.0 as eluent to perform elution and collecting eluent; and fourthly, concentrating the eluent to obtain the mulberry leaf phenolic acid extract. The mulberry leaf phenolic acid extract has high content of phenolic acid effective components and high activity. The invention further discloses the preparation method and application of the mulberry leaf phenolic acid extract.

Description

technical field [0001] The invention relates to a mulberry leaf extract, in particular to a mulberry phenolic acid extract with antibacterial activity, and also relates to a preparation process and application of the mulberry phenolic acid extract. Background technique [0002] Our country is extremely rich in mulberry resources, and mulberry leaves are included in the list of "items that are both food and medicine" by the Ministry of Health. It is not only one of the core raw materials of Guangdong herbal tea beverages, but also a traditional Chinese medicine for clearing away heat and detoxification widely used in clinical medicine. Studies have found that mulberry leaves are rich in phenolic acids, including chlorogenic acid, gallic acid, vanillic acid, caffeic acid, benzoic acid, astragaloside, rutin, catechin, syringic acid, gentisic acid, Tea element, catechol, guaiacol, quercetin, kaempferol, etc., the mulberry phenolic substances extracted from mulberry leaves have e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/38A23L1/29A23L33/00
Inventor 沈维治邹宇晓廖森泰刘凡肖更生施英穆利霞刘军
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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