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Method for catalytic synthesis of fructose lauric acid monoester by using immobilized phospholipase A1

A technology for immobilizing phospholipase and lauric acid monoester, applied in the field of fructose lauric acid monoester, can solve problems such as unseen, and achieve the effects of low toxicity, good industrial application prospect and easy separation

Inactive Publication Date: 2012-11-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the preparation of fructose laurate monoester from fructose and lauric acid catalyzed by immobilized phospholipase A1

Method used

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  • Method for catalytic synthesis of fructose lauric acid monoester by using immobilized phospholipase A1
  • Method for catalytic synthesis of fructose lauric acid monoester by using immobilized phospholipase A1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] a. Weigh 5 g of activated DA-201 macroporous adsorption resin, mix 10 g of phosphate buffer solution, and 1 g of free phospholipase A1, stir and adsorb at 25°C for 2 hours, and then filter to obtain macroporous adsorption resin immobilized phospholipase , its enzyme activity is 1850 U / g. Vacuum dried, ready for use;

[0026] b. Place the 4? molecular sieve in an oven at 105°C for 10 hours, then place it in a desiccator and cool it to room temperature for later use; after that, add the activated 4? in tert-butanol, sealed and placed for 72h, and filtered to remove molecular sieves to obtain a dehydrated organic solvent;

[0027] c. Enzymatic esterification: Mix 10g of lauric acid and 1.8g of fructose in the above-mentioned dehydrated organic solvent with a volume of 42ml, then add 2g of immobilized phospholipase A1 to start the reaction; after 2 hours of reaction, add 10g of activated 4? Generated water; shake or stir the reaction at 45°C for 48 hours, and the shakin...

Embodiment 2

[0029] a. Weigh 5 g of activated DA-201 macroporous adsorption resin, mix with 20 g of phosphate buffer, and 1 g of free phospholipase A1, stir and adsorb at 30°C for 4 hours, and then filter to obtain macroporous adsorption resin immobilized phospholipase. Its enzyme activity is 1462 U / g. Vacuum dried, ready for use;

[0030] b. Place the 4? molecular sieve in an oven at 100°C to activate for 8 hours, and then place it in a desiccator to cool to room temperature for later use; after that, add the activated 4? molecular sieve to the reaction medium in an amount of 60g per liter of solvent in tert-amyl alcohol, sealed for 24 hours, filtered to remove molecular sieves, to obtain a dehydrated organic solvent;

[0031] c. Enzymatic esterification: Mix 10g of lauric acid and 2.3g of fructose in the above-mentioned dehydrated organic solvent with a volume of 100ml, then add 2g of immobilized phospholipase A1 to start the reaction; add 10g of activated 4? Generated water; shake o...

Embodiment 3

[0033] a. Weigh 5 g of activated DA-201 macroporous adsorption resin, mix with 20 g of phosphate buffer, and 1 g of free phospholipase A1, stir and adsorb at 35°C for 3 hours, then filter to obtain macroporous adsorption resin immobilized phospholipase, Its enzyme activity is 1344 U / g. Vacuum dried, ready for use;

[0034] b. Place the 3? molecular sieve in an oven at 95°C to activate for 5 hours, and then place it in a desiccator to cool to room temperature for later use; after that, add the activated 3? molecular sieve to the reaction medium in an amount of 50 g per liter of solvent in acetone, sealed for 72 hours, and filtered to remove molecular sieves to obtain a dehydrated organic solvent;

[0035] c. Enzymatic esterification: Mix 40.6g of lauric acid and 36.5g of fructose in the above-mentioned dehydrated organic solvent with a volume of 812ml, then add 8.12g of immobilized phospholipase A1 to start the reaction; add 40.6g of activated 3? The water generated during ...

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Abstract

The invention discloses a method for catalytic synthesis of the fructose lauric acid monoester by using the immobilized phospholipase A1. According to the method, lauric acid and fructose are subjected to catalytic reaction through enzymes to synthesize the fructose lauric acid monoester. The preparation method includes the steps of immobilizing the phospholipase A1, preparing a dehydration organic solvent, and subjecting the immobilized phospholipase A1 to catalytic synthesis to produce the fructose lauric acid monoester. According to the method for catalytic synthesis of the fructose lauric acid monoester by using the immobilized phospholipase A1, the used phospholipase A1 is cheap in price and only 5% to 10% of the price of lipase, and the price of resin D-201 used for immobilizing the phospholipase is low. The immobilized phospholipase A1 has good catalytic effect, the conversion rate of the fructose lauric acid monoester is high, the product separation is simple, and the immobilized phospholipase A1 can be recycled. Accordingly, products prepared through the method are low in cost and have good application prospect in industry.

Description

technical field [0001] The invention relates to an immobilized phospholipase A1 catalyzing the synthesis of a sugar ester surfactant-fructose laurate monoester, which belongs to the field of organic synthesis. Background technique [0002] Sugar esters are generally formed by linking long-chain fatty acids and sugars through ester bonds. Therefore, sugar ester molecules have non-polar long-chain fatty acids and polar polyhydroxy sugars at the same time, thus having the effect of surfactant. Sugar ester molecules do not have ionizing functional groups and are typical nonionic surfactants. As a typical nonionic green biosurfactant with excellent performance, it has important uses in industries such as food, cosmetics, medicine, detergent, and fiber. Fructose esters, as a class of nonionic surfactants, also have a wide range of applications. [0003] There are chemical and enzymatic methods for the synthesis of sugar esters. The chemical method is often carried out under hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02
Inventor 赵谋明赵强忠崔春付敏刘宁仇超颖
Owner SOUTH CHINA UNIV OF TECH
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