Two-time scanning-based high-resolution optical scanning holographic section imaging method

Abstract

The invention discloses a two-time scanning-based high high-resolution optical scanning holographic section imaging method, belongs to the field of optical scanning and mainly overcomes the defect that larger defocus noise exists in the prior art when any two-dimensional sliced image is reconstructed. The two-time scanning-based high high-resolution optical scanning holographic section imaging method comprises the following steps of carrying out two-dimensional scanning on an object on a two-dimensional scanning mirror for the first time, moving the object towards the direction of the two-dimensional scanning mirror by a distance deltaZ after a first matrix equation containing section information is obtained and carrying out scanning on the object for the second time to obtain a second matrix equation containing the section information; and then integrating the first matrix equation and the second matrix equation into a minimum linear equation, converting the solution of a linear problem into a minimum problem and realizing section imaging through introducing a conjugate gradient algorithm. Through the technical scheme, the two-time scanning-based high high-resolution optical scanning holographic section imaging method has the beneficial effects that the high-precision section imaging is realized, and the defocus noise is greatly reduced. The two-time scanning-based high high-resolution optical scanning holographic section imaging method is suitable for various fields.

Description

technical field [0001] The invention belongs to the field of optical scanning, and in particular relates to a high-resolution optical scanning holographic slice imaging method based on two scanning. Background technique [0002] Optical scanning holography, referred to as OSH, is a non-traditional imaging technology based on Fresnel zone plate scanning, which achieves high-resolution three-dimensional imaging of targets through two-dimensional optical scanning. It is used in biomedical imaging and fluorescent object imaging. , 3D holographic television system and optical remote sensing and other fields have broad application prospects. [0003] The two-dimensional hologram obtained by optical scanning holography technology contains the complete three-dimensional information of the object. Therefore, an important analysis and processing step of the object hologram in optical scanning holography technology is the slice imaging of the object, that is, any two-dimensional sectio...

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Problems solved by technology

[0004] The literature "Optical Scanning Holography with MATLAB" proposes a traditional slice imaging method, which uses the hologram of the object and the Fresnel zone plate conjugate at the slice to be reconstructed to perform convolution operations to achieve slice imaging, but Its application is greatly restricted due to the inability to eliminate isolated noise

[0005] The document "Three-dimensional microscopy and sectional image reconstruction using optical scanning holography" introduces an inverse imaging algorithm. This iterative algorithm can achieve slice imaging with an axial resolution of about 1 mm, and can effectively suppress defocus noise, but its Cannot image well at smaller axial dimensions

[0006] The document "Depth resolution enhancement in optical scanning holography with a dual-wavelength laser source" proposes a method to improve the axial resolution of slice imaging by using a dual-wavelength laser. It uses lasers with output wavelengths of 632nm and 543nm respectively to obtain two sets of Object hologram, which in turn increases the axial resolution to about 2.5 microns, but its practicality is greatly limited due to the large noise introduced by the two different wavelengths working simultaneously in the optical system

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