Method for culturing high-fidelity lactobacillus of pickles and preparing preparation thereof

A lactic acid bacteria agent and technology of lactic acid bacteria, applied in the field of food processing, can solve the problems of single species of lactic acid bacteria and inability to fully reproduce the flavor of traditional kimchi, and achieve the effects of reduced nitrite content, obvious social and economic benefits, and shortened production cycle

Inactive Publication Date: 2012-12-05
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, the shortcoming of this technology also is that the fermented lactic acid bacte

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] The lactic acid bacteria in the kimchi are expanded and cultivated, and the lactic acid bacteria cells are separated by a centrifugal concentration separation method, and then spray-dried by adding a protective agent to prepare a high-fidelity lactic acid bacteria preparation.

[0022] Expand medium composition: 1000ml water, 10g whey powder, 10g peptone, 10g beef extract, 7g corn steep liquor, 6g glucose, 2g dipotassium hydrogen phosphate, 0.75g magnesium sulfate, 0.3g manganese sulfate, 1.5g sodium acetate, pH value is 6.4.

[0023] Centrifugation conditions: rotation speed 4000rpm, centrifugation time 15min.

[0024] Spray drying conditions: air inlet temperature 130°C, feed flow rate 12.54ml / min, ratio of gum arabic to maltodextrin 1:8, wall material content about 20%, dosage of heat-resistant protective agent 6g.

[0025] After identification, the main strains of high-fidelity compound lactic acid bacteria are:

[0026] 1# colony is white, round, small, round, in...

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Abstract

The invention provides a high-fidelity lactobacillus preparation used for fermenting Sichuan pickles and a method for preparing the high-fidelity lactobacillus preparation. The method comprises following steps: (1) amplification culture of pickle lactobacillus is conducted, wherein a formula of a co-culture medium of the pickle lactobacillus is that 8g to 14g of whey powder, 8g to 14g of peptone, 8g to 14g of beef extract, 5g to 12g of corn steep liquor, 4g to 8g of glucose, 2g to 3g of dipotassium hydrogen phosphate, 0.5g to 1.0g of magnesium sulfate, 0.1g to 0.5g of manganese sulfate and 0.5g to 2.5g of sodium acetate are added to every 1000mL of water, and multiple lactobacilli in traditional pickle fermentation water are co-cultured in the co-culture medium by adopting a strain co-culture technology; (2) a high-fidelity lactobacillus cell solution is eccentrically concentrated and separated; and (3) the high-fidelity lactobacillus cell solution is sprayed and dried at low temperature. By using the high-fidelity lactobacillus preparation provided by the invention during fermentation production of the Sichuan pickles, the flavor of the traditional Sichuan pickles is retained; and compared with a traditional process, the high-fidelity lactobacillus preparation has the advantages that the production period is shortened by 25 percent to 25 percent, the use amount of salt is reduced by 25 percent to 40 percent than the original process, the total acidity of a product is increased by 20 percent to 40 percent and the nitrite content is reduced by 15 percent to 30 percent, so that the high-fidelity lactobacillus preparation has obvious social benefits and economic benefits.

Description

Technical field: [0001] The application belongs to the technical field of food processing, and relates to an improved kimchi high-fidelity lactic acid bacteria preparation and a preparation method of the kimchi high-fidelity lactic acid bacteria preparation. Background technique: [0002] "The world's kimchi looks at China, and the Chinese kimchi looks at Sichuan." Kimchi is a traditional fermented vegetable processing product with a long history in my country, and it is also one of the most characteristic and advantageous industries in Sichuan Province. At present, most of the starters used in the production of traditional fermented kimchi stay on the original material starters for self-propagation and self-use. In a series of complicated processes such as strain propagation and propagation, due to backward technology and equipment, strains are easy to degrade and mutate. , in actual production, only rely on simple inoculation of raw materials and finished products and nat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225C12R1/01
Inventor 车振明向文良贾春邢亚阁张琦龚丽曾亮唐洁刘洪李可
Owner XIHUA UNIV
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