Method for culturing high-fidelity lactobacillus of pickles and preparing preparation thereof
A lactic acid bacteria agent and technology of lactic acid bacteria, applied in the field of food processing, can solve the problems of single species of lactic acid bacteria and inability to fully reproduce the flavor of traditional kimchi, and achieve the effects of reduced nitrite content, obvious social and economic benefits, and shortened production cycle
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[0021] The lactic acid bacteria in the kimchi are expanded and cultivated, and the lactic acid bacteria cells are separated by a centrifugal concentration separation method, and then spray-dried by adding a protective agent to prepare a high-fidelity lactic acid bacteria preparation.
[0022] Expand medium composition: 1000ml water, 10g whey powder, 10g peptone, 10g beef extract, 7g corn steep liquor, 6g glucose, 2g dipotassium hydrogen phosphate, 0.75g magnesium sulfate, 0.3g manganese sulfate, 1.5g sodium acetate, pH value is 6.4.
[0023] Centrifugation conditions: rotation speed 4000rpm, centrifugation time 15min.
[0024] Spray drying conditions: air inlet temperature 130°C, feed flow rate 12.54ml / min, ratio of gum arabic to maltodextrin 1:8, wall material content about 20%, dosage of heat-resistant protective agent 6g.
[0025] After identification, the main strains of high-fidelity compound lactic acid bacteria are:
[0026] 1# colony is white, round, small, round, in...
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