Compound premix for pigs and mixed batch of compound premix
A technology for compounding premix and raw materials, applied in application, animal feed, animal feed, etc., can solve the problems of insignificant probiotic effect, limited application effect, intolerance to antibiotics, etc., to reduce the incidence, avoid mutual resistance, and relieve Effects of heavy metal poisoning
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Embodiment 1
[0021] Embodiment 1 Selenium-enriched yeast powder preparation
[0022] (1) Incline strain: inoculate the strain of Saccharomyces cerevisiae CGMCC No.0539 (purchased from the Institute of Microbiology, Chinese Academy of Sciences) in wort containing 15% sugar and 500 μg / ml sodium selenite On a solid slant, culture at 28°C for 36 hours, and store in a 4°C refrigerator.
[0023] (2) First-level liquid strain culture: After activating the preserved selenium-enriched yeast strain, a ring of cells was cultured in 150 ml of wort with a total sugar content of 15% and a selenite concentration of 500 μg / ml Under the condition of 28° C., the rotation speed of the shaker is 180 rpm, and cultivated for 20 hours, which is the first-level liquid seed culture.
[0024](3) Secondary liquid strain culture: Put the primary seed culture solution into a 3000ml Erlenmeyer flask of wort medium with a total sugar content of 15% and a selenite concentration of 500 μg / ml at an inoculum size of 10%. ...
Embodiment 2
[0029] Embodiment 2 Preparation of iron-rich yeast powder
[0030] (1) Incline strains: inoculate iron-rich yeast (Saccharomyces cerevisiae) CGMCC No.0895 (purchased from the Institute of Microbiology, Chinese Academy of Sciences) on wort solids containing 8% sugar and 400 μg / ml ferrous sulfate After culturing for 48 hours on the slant at 28°C, store in a 4°C refrigerator.
[0031] (2) First-level liquid strain culture: After activating the preserved iron-rich yeast strains, connect a ring of cells to 150 ml of wort medium with a total sugar content of 10% and a ferrous sulfate concentration of 400 μg / ml In the Erlenmeyer flask, under the condition of 28 ℃, the rotation speed of the shaker is 180rpm, and cultivated for 18 hours, which is the first-level liquid seed culture.
[0032] (3) Secondary liquid strain culture: Put the primary seed culture solution into a 3000ml Erlenmeyer flask of wort medium with a total sugar content of 20% and a ferrous sulfate concentration of 60...
Embodiment 3
[0037] Embodiment 3 Zinc-rich yeast powder preparation
[0038] (1) Slant strains: inoculate the strains of zinc-rich yeast (Saccharomyces cerevisiae) CGMCC No.0896 (purchased from the Institute of Microbiology, Chinese Academy of Sciences) on wort solid slopes containing 10% sugar and 400 μg / ml zinc sulfate concentration After culturing for 48 hours at 28°C, store in a 4°C refrigerator.
[0039] (2) First-level liquid strain culture: After activating the preserved zinc-rich yeast strains, connect a ring of cells to a medium containing 150 ml of wort medium with a total sugar content of 10% and a zinc sulfate concentration of 400 μg / ml. In the Erlenmeyer flask, under the condition of 28° C., the rotating speed of the shaker is 180 rpm, and cultured for 16 hours, which is the primary liquid seed culture.
[0040] (3) Secondary liquid strain culture: Put the first-level seed culture solution into a 3000ml Erlenmeyer flask of wort medium with a total sugar content of 12% and a z...
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