High performance liquid chromatography for simultaneously detecting content of ten organic acids in fruit

A high-performance liquid chromatography and organic acid technology, applied in the field of content determination, to achieve the effect of simple and fast operation and high measurement accuracy

Inactive Publication Date: 2012-12-12
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
View PDF2 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in the prior art, 10 organic acids (oxalic acid, citric acid, tartaric acid, succinic acid, malic acid,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High performance liquid chromatography for simultaneously detecting content of ten organic acids in fruit
  • High performance liquid chromatography for simultaneously detecting content of ten organic acids in fruit
  • High performance liquid chromatography for simultaneously detecting content of ten organic acids in fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] dried grape peel

[0033] (1) Preparation of standard sample solution: Weigh oxalic acid, citric acid, tartaric acid, succinic acid, malic acid, fumaric acid, acetic acid, propionic acid, isopropionic acid, butyric acid reagents with HPLC chromatographic purity above 99%, Use 0.001mol / L sulfuric acid solution to prepare 0.5mg / mL standard solutions of 10 kinds of organic acids; according to the actual content of organic acids in wine and the response value obtained at the maximum absorption wavelength of each standard, dilute a certain number of times to prepare a mixed standard solution , and then dilute the mixed standard solution into different concentration gradients to obtain standard sample solutions, that is, 0.5, 0.25, 0.1, 0.05, 0.025, and 0.02 times the concentration of the above mixed standard solution.

[0034] (2) Preparation of the solution to be tested: Weigh 2g of dried grape peel and grind it with 20mL of 80% ethanol, then extract it in a constant temper...

Embodiment 2

[0050] dried grape pulp

[0051] The only difference between this example and Example 1 is that the dried grape pulp sample is used instead of the dried grape peel sample in Example 1; other operations are completely consistent with Example 1. This embodiment detects and obtains 10 standard curve regression equations (see above table 1), such as figure 1 The high performance chromatograms of the standard samples shown and image 3 The high-efficiency chromatogram of the liquid to be tested is shown; the content of 10 organic acids in the dried grape pulp sample is quantitatively analyzed and calculated by the external standard method, and the analysis and calculation results are shown in Table 2 above.

Embodiment 3

[0053] fresh loquat pulp

[0054] (1) Preparation of standard sample solution: Weigh oxalic acid, citric acid, tartaric acid, succinic acid, malic acid, fumaric acid, acetic acid, propionic acid, isopropionic acid, butyric acid reagents with HPLC chromatographic purity above 99%, Use 0.0025mol / L sulfuric acid solution to prepare 0.5mg / mL standard solutions of 10 kinds of organic acids; according to the actual content of organic acids in wine and the response value obtained at the maximum absorption wavelength of each standard, dilute a certain number of times to prepare a mixed standard solution , and then dilute the mixed standard solution into different concentration gradients to obtain standard sample solutions, that is, 0.5, 0.25, 0.1, 0.05, 0.025, and 0.02 times the concentration of the above mixed standard solution.

[0055] (2) Preparation of the solution to be tested: Weigh 10g of fresh loquat pulp and place it in a tissue masher, add 100ml of 80% ethanol to homogenate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a high performance liquid chromatography for simultaneously detecting the content of ten organic acids in a fruit, comprising the following steps of: carrying out high performance liquid chromatography detection on a prepared standard sample solution and a solution to be detected under the following chromatography conditions: a hydrogen ion exchange column is adopted as an analysis column, flowing phases include a 0.001-0.005mol/L sulfuric acid solution as a water phase A and acetonitrile as an organic phase B, wherein the volume percentage of A is 100-96%, the volume percentage of B is 4-0%, the flow rate is 0.4-0.5mL/min, isocratic elution is used as an elution form, the detection wavelength is 210-280nm, the detection temperature is 25-50 DEG C, the feeding volume is 10-20muL, and the detection time is 30min; and finally, quantitatively analyzing and calculating the content of the ten organic acids in the fruit by using an external standard method. The method has the following characteristics of easiness and fastness in operation.

Description

【Technical field】 [0001] The invention relates to a method for measuring the content of organic acids in fruits, in particular to a high-performance liquid chromatography method for simultaneously measuring the contents of 10 kinds of organic acids in fruits. 【Background technique】 [0002] Organic acids refer to some organic compounds with acidic properties. The most common organic acids are carboxylic acids, which derive their acidity from the carboxyl group (-COOH). Sulfonic acid (-SO 3 H), sulfinic acid (RSOOH), thiocarboxylic acid (RCOSH), etc. also belong to organic acids. Organic acids can react with alcohols to form esters. Carboxyl is the functional group of carboxylic acid. Except for formic acid (H-COOH), carboxylic acid can be regarded as the derivative of hydrogen atom in hydroxyl molecule replaced by carboxyl. It can be represented by the general formula (Ar) R-COOH. Carboxylic acids often exist widely in nature in the free state or in the form of salts an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/88G01N30/12
Inventor 陈源余亚白何志刚林晓姿魏巍谢鸿根
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap