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Method for screening GHSR1a agonist drug

A screening method and agonist technology, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as interference with screening effects, stimulation of growth hormone secretion, and instability

Active Publication Date: 2012-12-12
辉源生物科技(上海)有限公司
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Problems solved by technology

However, the secretion of growth hormone is not stable due to the influence of various environmental, pathological or non-pathological factors
For example, external stimuli (temperature, noise, foreign body contact, etc.), surgical stress, heat sources or the use of large doses of vasopressin can stimulate the secretion of growth hormone and interfere with the screening effect

Method used

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  • Method for screening GHSR1a agonist drug
  • Method for screening GHSR1a agonist drug
  • Method for screening GHSR1a agonist drug

Examples

Experimental program
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Effect test

Embodiment Construction

[0021] 1. Animal feeding

[0022] Male C57 / BL6J mice, 20-25 g, were raised at SPF level, room temperature was 22.7±0.8°C, humidity was 60%, and a 12:12-hour day-night cycle was adopted. After entering the field, they were adaptively fed for one week, and divided into random groups, with 6 animals in each group and 2 animals in each cage. Animal use strictly complies with ethical standards for animal care and use.

[0023] 2. The establishment of a stable method for growth hormone secretion

[0024] Two groups of animals were respectively given GHSR1a agonist ghrelin (1 mg / kg, intraperitoneal injection) or normal saline as solvent control. 30 μL of blood was collected from the two groups at 5, 15, 30, 60 and 180 minutes after administration. 10 μL of plasma was separated, and the growth hormone content in plasma was determined using Rat / Mouse GH ELISA kit. The result is as figure 1 As shown, it is difficult to accurately judge the effect of GHSR1a agonists due to the unsta...

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Abstract

A method for screening a GHSR1a agonist drug mainly comprises the following steps of: 1) carrying out prestimulation for multiple times before administration so as to stabilize basic level of growth hormone; 2) respectively drawing 30 microliters of blood 5, 15, 30, 60, 180 and 300 minutes after administration; 3) separating 10 microliters of blood plasma and using a Rat / Mouse GH ELISA kit to determine the content of the growth hormone in the blood plasma; and 4) choosing a drug, which enables the area rising rate under a blood plasma growth hormone concentration-time curve to be greater than 50%. According to the method for screening the GHSR1a agonist drug, a screening model of the stable, sensitive and repeatable GHSR1a agonist drug is established by the means of assumptive blood collection before administration. The invention can provide a reliable and effective method for screening the GHSR1a agonist drug in animal body.

Description

technical field [0001] The invention relates to a drug screening method, in particular to a GHSR1a agonist drug screening method. Background technique [0002] In recent years, the research and development of growth hormone secretagogue receptor 1a (growth hormone secretagogue receptor, GHSR1a) selective ligands has become a hot spot, which has proved to have broad clinical application prospects. GHSR1a agonist can stimulate appetite and growth hormone release, so it can be used for the treatment of anorexia and cachexia. GHSR1a agonist also has anti-inflammatory, anti-oxidation, inhibition of apoptosis, regulation of sleep and memory, etc. Muscular dystrophy associated with old age or degenerative diseases, etc. It is particularly important to establish a sensitive and stable GHSR1a selective agonist animal screening model based on its mechanism of action. However, the secretion of growth hormone is not stable due to the influence of various environmental, pathological or...

Claims

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Application Information

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IPC IPC(8): G01N33/00
Inventor 张宝弘高炜炜
Owner 辉源生物科技(上海)有限公司
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