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Method for culturing horseradish callus through taking horseradish root as explant

A technology of callus and culture method, which is applied in the field of tissue culture of wasabi plants, to achieve the effect of high content of active ingredients, strong efficacy, and satisfying needs

Active Publication Date: 2012-12-19
广元市玺府生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing people have carried out research to the tissue culture of wasabi plant at present, as: the paper " wasabi leaf callus induction test " of Wang Anhu, the paper " research of culture of wasabi callus and flavor substance thereof " of Zhang Jianjia and the paper " of wasabi leaf " Wasabi in vitro culture research", etc., but the explants used in the wasabi callus induction culture are mainly the leaves, petioles and buds of the wasabi plant, and there is no callus induction using the wasabi root as the explant cultivated reports

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Treatment of explants: Take wasabi plants that are free of bacteria and grow healthy, cut off their roots, rinse the wasabi roots with running water, put them on filter paper to absorb the surface moisture, and then put them in an ultra-clean Disinfect the workbench with 0.1% mercuric chloride for 7 minutes, rinse it with sterile water for 5 times, and dry the surface with sterilized filter paper;

[0017] (2) Induction culture of callus: cut the sterilized wasabi root into a length of 1.5cm, and then put it into the callus culture medium MS+ 6-BA1.0mg·L -1 +2,4-D4.0mg·L -1 +IAA2.0mg·L -1 The culture conditions were: temperature 18°C, light for 8 hours a day, light intensity 1000lx, and after 30 days of culture, the callus induction rate was 92%; it was observed that the callus was first produced at the incision at both ends of the root At first, yellow-green callus is produced, and with the growth of culture time, yellow-white callus can also be produced;

[00...

Embodiment 2

[0021] (1) Treatment of explants: Take wasabi plants that are free of bacteria and grow healthy, cut off their roots, rinse the wasabi roots with running water, put them on filter paper to absorb the surface moisture, and then put them in an ultra-clean Disinfect the workbench with 0.1% mercuric chloride for 8 minutes, rinse it with sterile water for 5 times, and dry the surface with sterilized filter paper;

[0022] (2) Induction culture of callus: cut the root of the sterilized wasabi into a length of 2 cm, and then insert it into the callus culture medium MS+6-BA3.0mg L -1 +2,4-D2.0mg·L -1 +IAA1.0mg·L -1 The culture conditions were: temperature 25°C, light for 6 hours per day, and light intensity 1500lx. After 30 days of culture, the callus induction rate was 87%. It was observed that the callus was mainly produced in the free end;

[0023] (3) Proliferation culture of callus: transfer the yellow-green callus obtained in step (2) into callus proliferation medium 1 / 2MS+6-...

Embodiment 3

[0026] (1) Treatment of explants: Take wasabi plants that are free of bacteria and grow healthy, cut off their roots, rinse the wasabi roots with running water, put them on filter paper to absorb the surface moisture, and then put them in an ultra-clean Disinfect the workbench with 0.15% mercuric chloride for 5 minutes, rinse it with sterile water for 4 times, and dry the surface with sterilized filter paper;

[0027] (2) Induction culture of callus: Cut the root of the sterilized wasabi into a length of 1.5 cm, and then put it into the callus culture medium MS+6-BA1.5 mg L in a flat manner -1 +2,4-D5.0mg·L -1 +IAA2.5mg·L -1 Induction culture was carried out in the medium, and the culture conditions were: temperature 20°C, 10 hours of light per day, and light intensity of 1000lx. After 30 days of culture, the callus induction rate was 90%;

[0028] (3) Proliferation culture of callus: transfer the callus prepared in step (2) into callus proliferation medium 1 / 2MS+2,4-D3.0mg·...

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Abstract

The invention discloses a method for culturing horseradish callus through taking the horseradish root as an explant, which is characterized in that the horseradish root is taken as the explant to be grafted into a callus culture medium to culture, and the horseradish callus with high active ingredient content is successfully induced. Therefore, the problem of horseradish plant resource shortage can be effectively solved, the market demands on horseradish products are satisfied, and the produced horseradish downstream products have stronger effects and better tastes.

Description

technical field [0001] The invention relates to a method for tissue culture of wasabi plants, in particular to a method for culturing wasabi callus with wasabi roots as explants. Background technique [0002] Wasabi (Wasabijaponica Matsum), also known as bean vegetable and mountain vegetable, is a perennial semi-shady herbaceous plant of the genus Brassicaceae. [0003] The wasabi plant contains a substance called sinapin, which is hydrolyzed under the action of myrosinase to form isothiocyanate compounds when the plant is ground, especially the content of allyl isothiocyanate The highest, accounting for about 89% to 94%. In addition, the wasabi plant also contains a large amount of amino acids and various trace elements needed by the human body. Because wasabi is rich in isothiocyanate compounds and a large amount of amino acids and trace elements needed by the human body, wasabi not only has a strong spicy taste and a special spicy taste, but also has immune regulation, a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王跃华任三军赵钢孙雁霞刘洪明李亚梅李琴冯玉艺任强
Owner 广元市玺府生物科技有限公司
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