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Method for preparing parthenolide originated from magnolia delavayi, magnolia liliiflora and magnolia denudate

A technology of parthenolide and magnolia, which is applied in the direction of organic chemistry, can solve the problems of cumbersome process, high production cost, and not too high parthenolide content, and achieve the effect of easy industrialization and simple extraction and separation process

Active Publication Date: 2013-01-09
ACCENDATECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Patent CN101190258 discloses a method of extracting rich parthenolide extract from leaves or flowers of Magnolia Magnolia, but the content of parthenolide in leaves or flowers of Magnolia Magnolia used in the method is not too high (content of about 0.3%), and the process is more cumbersome and the production cost is higher

Method used

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  • Method for preparing parthenolide originated from magnolia delavayi, magnolia liliiflora and magnolia denudate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Isolation and purification of parthenolide from the root bark of Yulan magnolia

[0027] Take 1 kg of dry root bark of Magnolia japonica, and grind it into powder. Get the pulverized coarse powder, extract 3 times with 10 times (weight) of 95% ethanol in reflux, each time for 3 hours. Filtrate, combine the filtrates, and recover the solvent under reduced pressure to obtain the extract. The extract was dissolved with 20% ethanol, then extracted with ethyl acetate until the color of the extract became lighter, the organic solvent was combined, the solvent was recovered under reduced pressure, and dried to obtain 114 grams of solid. Put on a silica gel chromatography column, elute with petroleum ether: ethyl acetate = 15:1 ~ 4:1 gradient, TLC tracking detection, compare with standard parthenolide, collect fractions containing parthenolide, combine and reduce pressure The solvent was recovered to obtain a dry solid. Crystallization in a mixed solvent of petrol...

Embodiment 2

[0031] Example 2: Isolation and Purification of Parthenolide from Magnolia Root Bark

[0032] Take 1 kg of dry root bark of Magnolia japonica, and crush it into powder. Get the pulverized coarse powder, extract 3 times with 10 times (weight) of 95% ethanol in reflux, each time for 3 hours. Filtrate, combine the filtrates, and recover the solvent under reduced pressure to obtain the extract. The extract was dissolved with 20% ethanol, then extracted with ethyl acetate until the color of the extract became lighter, the organic solvent was combined, the solvent was recovered under reduced pressure, and dried to obtain 102 grams of solid. Put on a silica gel chromatography column, elute with petroleum ether: ethyl acetate = 15:1 ~ 4:1 gradient, TLC tracking detection, compare with standard parthenolide, collect fractions containing parthenolide, combine and reduce pressure The solvent was recovered to obtain a dry solid. Crystallization in a mixed solvent of petroleum ether: ac...

Embodiment 3

[0033] Example 3: Isolation and purification of parthenolide from magnolia root bark

[0034] Take 1 kg of dry root bark of Magnolia, and grind it into powder. Get the pulverized coarse powder, extract 3 times with 10 times (weight) of 95% ethanol in reflux, each time for 3 hours. Filtrate, combine the filtrates, and recover the solvent under reduced pressure to obtain the extract. The extract was dissolved with 20% ethanol, then extracted with ethyl acetate until the color of the extract became lighter, the organic solvent was combined, the solvent was recovered under reduced pressure, and dried to obtain 112 grams of solid. Put on a silica gel chromatography column, elute with petroleum ether: ethyl acetate = 15:1 ~ 4:1 gradient, TLC tracking detection, compare with standard parthenolide, collect fractions containing parthenolide, combine and reduce pressure The solvent was recovered to obtain a dry solid. Crystallization in a mixed solvent of petroleum ether: acetone = 1...

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Abstract

The invention relates to a method for extracting parthenolide, in particular to a method for extracting parthenolide from root barks of magnolia delavayi, magnolia liliiflora and magnolia denudate. Parthenolide is obtained by using the root barks of magnolia delavayi, magnolia liliiflora and magnolia denudate as raw materials, grinding, extracting with polar organic solvent, properly extracting with organic solvent to obtain total lactone, separating total lactone by column chromatography, collecting necessary components, and crystallizing with proper solvent. The method for extracting parthenolide from root barks of magnolia delavayi, magnolia liliiflora and magnolia denudate provided by the invention is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of medicine extraction. Specifically, the invention relates to a preparation method of parthenolide, in particular to a method for extracting parthenolide from the root bark of mountain magnolia, purple magnolia and white magnolia. Background technique [0002] Tumors are a great threat to human health. There are about 2 million cancer patients in my country, with 1.6 million new cases every year. This is a large group. Anti-tumor research is a very challenging and significant field in the field of life sciences today. . In the past, the treatment methods focused on the eradication and killing of cancer cells. At present, the commonly used antineoplastic drugs in clinical practice are mainly cytotoxic drugs. These anticancer drugs have disadvantages such as poor selectivity, strong toxic and side effects, and easy drug resistance. , is a typical double-edged drug, and it is difficult to eradicate cancer, and...

Claims

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Application Information

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IPC IPC(8): C07D493/04
Inventor 张泉陈悦范洪霞龙菁翟佳黛
Owner ACCENDATECH
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