Fusion tandem antibacterial peptide and preparation method thereof
A technology for antimicrobial peptides and small molecules, applied in the field of antimicrobial peptides and their preparation, can solve the problems of high production cost, difficult to express, low expression amount, etc., and achieve the effects of low cost, improved bacteriostatic activity and high expression amount
Inactive Publication Date: 2013-01-09
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology
[0008] The present invention aims to solve the problem of low yield and high production cost in the existing antimicrobial peptide synthesis method; especially the antimicrobial peptide molecule is easily hydrolyzed by enzymatic hydrolysis in the process of recombinant expression of antimicrobial peptide by genetic engineering, resulting in low or difficult expression. Fusion tandem antimicrobial peptide and preparation method thereof
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specific Embodiment approach 1
[0027] Specific embodiment 1: In this embodiment, the fusion tandem antimicrobial peptides are composed of four antibacterial peptides in series: Fowlicidin-2, cecropin B, CecropinA (1-8)-Melittin (1-18) and death factor. Fowlicidin-2, cecropin GlySerGly is used to link the protein B, CecropinA(1-8)-Melittin(1-18) and death factor.
specific Embodiment approach 2
[0028] Specific embodiment 2: The amino acid sequence of the fusion tandem antimicrobial peptide in this embodiment is shown in SEQ ID NO:2.
specific Embodiment approach 3
[0029] Specific embodiment three: The amino acid sequence of the fused tandem antimicrobial peptide in this embodiment is shown in SEQ ID NO:4.
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The invention discloses a fusion tandem antibacterial peptide and a preparation method thereof, relates to an antibacterial peptide and a preparation method thereof and solves problems that existing antibacterial peptide synthetic methods are low in yield and high in production cost and particularly the expression quantity is low or the expression is difficult caused by the fact that antibacterial peptide molecules are prone to be subjected to enzymolysis during the recombinant expression of the antibacterial peptide by genetic engineering. Four types of antibacterial peptides of fowlicidin-2, cecropin B, cecropin a (1-8)-melittin (1-18) and thanatin are in a series connection to form the fusion tandem antibacterial peptide. The preparation method includes (1) designing a cDNA fragment; (2) constructing a recombinant expression vector; (3) obtaining a transformant; and (4) inducing, collecting a supernatant and performing protein purification. The preparation method is used for the field of antibacterial peptide preparation.
Description
technical field [0001] The present invention relates to antibacterial peptide and its preparation method. Background technique [0002] Antimicrobial peptides are biologically active small molecular peptides produced by the immune defense system of organisms under the stimulation of immune pathogens. They are important components of the non-specific immune systems of animals and plants, and are widely distributed in bacteria, fungi, higher plants, insects, amphibians, mammals and humans. Antimicrobial peptides have antibacterial, antiviral, antitumor, and immunomodulatory effects. Compared with antibiotics, antimicrobial peptides have the advantages of small molecular weight, broad antibacterial spectrum, good water solubility, strong heat resistance, non-immunogenicity and unique mechanism of action, and are not easy to cause microbial resistance. Healthy and safe antibacterial substances with potential application. [0003] Fowlicidin-2 is a new type of cationic antimic...
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IPC IPC(8): C07K19/00C12N15/81C12R1/84
Inventor 冯兴军李仲玉程宝晶李静
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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