Method for promoting fermentation of microorganism to produce 1,3-propylene glycol

A technology of microbial fermentation and propylene glycol, which is applied in the field of bioengineering, can solve the problems of inconspicuous fermentation effects and ineffective effects, and achieve the effects of reducing the generation of side reactants, reducing side reactions, and improving biological efficiency

Active Publication Date: 2013-01-09
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the addition of nutrient conditions in the optimized medium can improve the growth of bacteria and the level of fermentation, the intake of many nutrient molecules depends entirely on the needs of biological metabolism, such as the organic intermediate molecules in the TCA cycle in the anaerobic pathway.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Seed liquid culture: Take 1 mL of the liquid-preserved Klebsiella pneumoniae strain preservation solution and add it to 400 mL of seed medium, mix it in a 1L fermenter, and carry out seed liquid culture;

[0026] The culture conditions were controlled as follows: the culture temperature was 37°C, the stirring speed was set at 300 rpm, the pH was controlled at 7.0, the anaerobic conditions were maintained during the culture process, and the nitrogen influx was 2vvm.

[0027] (2) Fermentation culture: Batch fermentation mode is adopted, the volume of the fermentation tank is 15L, and the volume of the upper tank is 7L. Specific steps are as follows:

[0028] a, get 400mL seed liquid and join in 6600mL fermented medium that contains pyruvate, the concentration of pyruvate in fermented medium is 0.11g / L;

[0029] b. During the fermentation process, the culture temperature is controlled to be 37°C, the stirring speed is 400rpm, and 40% Ca(OH) 2 Adjust the pH so that it...

Embodiment 2

[0033] (1) Seed liquid culture: Take 2 mL of the liquid-preserved Klebsiella pneumoniae strain preservation solution and add it to 500 mL of seed medium, mix it in a 1L fermenter, and carry out seed liquid culture;

[0034] The culture conditions were controlled as follows: the culture temperature was 35°C, the stirring speed was set at 300 rpm, the pH was controlled at 7.0, the anaerobic conditions were maintained during the culture process, and the nitrogen influx was 3vvm.

[0035] (2) Fermentation culture: Batch fermentation mode is adopted, the volume of the fermentation tank is 15L, and the volume of the upper tank is 7L. Specific steps are as follows:

[0036] a. Take 500mL of seed liquid and add it to 6500mL of fermentation medium containing pyruvate, the concentration of α-ketoglutarate in the fermentation medium is 0.32g / L;

[0037] b. During the fermentation process, the culture temperature is controlled to be 35°C, the stirring speed is 400rpm, and 40% Ca(OH) 2 A...

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PUM

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Abstract

The invention discloses a method for promoting fermentation of microorganism to produce 1, 3-propylene glycol. The method comprises a seed liquid cultivation process and a fermentation process, wherein fermentation cultivation medium used in the fermentation process contains pyroracemic acid of which the concentration is 0.1-0.4g/L and/or alpha-oxoglutarate; the fermentation process orderly comprises micro-oxygen fermentation and anaerobic fermentation, and the micro-oxygen fermentation is converted into the anaerobic fermentation when an OD value is greater than 7. According to the method, conversion of glycerol can be effectively promoted, and the concentration of the 1, 3-propylene glycol in a final product is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and mainly relates to a method for promoting microbial fermentation to produce 1,3-propanediol. Background technique [0002] 1,3-Propanediol (1,3-Propanediol, PDO) is mainly used in the production of new polyester PTT (Polytrimethylene Terephthalate) with excellent performance. PTT not only has the chemical stability of PET, but also has the good elastic recovery performance and pollution resistance of nylon. With the development of low-cost 1,3-propanediol production process, PTT has broad application prospects in the field of textiles. In addition, 1,3-propanediol is also an important chemical raw material and pharmaceutical intermediate. It is widely used in the production of polyester fiber and the manufacture of polyurethane and cyclic compounds. It can replace ethylene glycol in the production of other polyesters. Esters (such as polytrimethylene naphthalate, PTN), preparation of ...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12R1/22C12R1/01
Inventor 张霖师文静李晓姝王领民
Owner CHINA PETROLEUM & CHEM CORP
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