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Method for continuously preparing pumpkin protein, ethanol fuel and dietary fiber

A technology of dietary fiber and fuel ethanol, applied in the direction of microorganism-based methods, biochemical equipment and methods, food preparation, etc., can solve the problems of resource waste, etc., and achieve the effects of easy industrialization, broad application prospects, and simple operation

Inactive Publication Date: 2013-12-04
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, patents CN102524698A and CN101195835A can only separate and utilize one active ingredient, and other active ingredients have to be removed as impurities in the purification process, resulting in a great waste of resources

Method used

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  • Method for continuously preparing pumpkin protein, ethanol fuel and dietary fiber
  • Method for continuously preparing pumpkin protein, ethanol fuel and dietary fiber
  • Method for continuously preparing pumpkin protein, ethanol fuel and dietary fiber

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Embodiment Construction

[0009] The embodiments of the present invention are described in detail below.

[0010] A method for continuously preparing pumpkin protein, fuel ethanol and dietary fiber, including washing, peeling, drying, and pulverizing the waste pumpkin pulp for seed use; ① Use 1 to 5% NaOH solution to pulverize the waste pumpkin pulp The material ratio is 20:1~30:1, the extraction temperature is 60~80 ℃, and the extraction time is 50~70 min. Under the conditions of constant temperature shaking, centrifugal separation, and filtration, the filtrate and the filter residue are obtained; ②The pH value of the obtained filtrate is adjusted to The protein isoelectric point is centrifuged to obtain the precipitate and supernatant. The precipitate is washed and freeze-dried to obtain pumpkin protein; ③The supernatant obtained in step ② is concentrated and alcohol-precipitated to obtain water-soluble dietary fiber; ④Step ① After washing the filter residue obtained in the middle filtration with water,...

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Abstract

The invention relates to a method for continuously preparing pumpkin protein, ethanol fuel and dietary fiber, belonging to a technique for preparing active substances from natural substances. The method comprises the following steps: oscillating a pumpkin pulverized material solution at constant temperature, centrifuging, and filtering to obtain a filtrate and a filter residue; regulating the pH value of the filtrate to a protein isoelectric point by using a buffer solution, carrying out centrifugal separation to obtain a precipitate and a supernatant, carrying out washing and freeze-drying on the precipitate to obtain the pumpkin protein, and carrying out concentration and alcohol precipitation on the supernatant to obtain the water-soluble dietary fiber; and washing the pumpkin filter residue with water, adding water, sequentially carrying out alpha-amylase liquification and saccharifying enzyme saccharification, carrying out centrifugal separation to obtain a precipitate and a supernatant, washing the precipitate with water, carrying out freeze-drying to obtain the water-insoluble dietary fiber, carrying out fermentation treatment on the supernatant with active dry yeast, filtering, distilling the filtrate, and dehydrating with a desiccant to obtain the ethanol fuel. The invention has the advantages of no mutual interference of various steps, high product yield, simple technological operation and low cost, and can easily implement industrial production.

Description

Technical field [0001] The invention belongs to the technology of preparing active substances from natural substances, and mainly relates to a method for continuously preparing pumpkin protein, fuel ethanol and dietary fiber by using waste pumpkin pulp as raw materials. Background technique [0002] pumpkin( Cucurbita moschata Duch) is the squash fruit of the trailing vine of the Cucurbitaceae family. my country is rich in pumpkin resources. The planting area of ​​pumpkins ranks second in the world, and the total output ranks first in the world, of which 20% is for food and 80% is pumpkin for seeds. Pumpkin has high nutritional value. It is rich in various nutrients such as amino acids, protein, cellulose, vitamins and trace elements. It also contains bioactive ingredients with health care functions such as polysaccharides and carotenoids. It is mainly used for anti-tumor, lowering blood sugar, and lowering blood sugar. Blood lipids, prevention of prostate diseases, and immune ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14C12P7/08A23L1/308C12R1/865
Inventor 徐雅琴杨昱王丽波梁宗言
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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