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Method for detecting antibacterial drugs of furazolidone and furacilin through surface-enhanced raman spectroscopy

A surface-enhanced Raman and furazolidone technology, which is used in the field of feed, food, and drug testing, can solve problems such as residues, low sensitivity, and ineffective antibacterial drugs.

Active Publication Date: 2015-06-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, the abuse of antibacterial drugs has become a public health problem of global concern. The harm is mainly due to the enhancement of drug resistance in the human body, which leads to the failure of antibacterial drugs. At the same time, the accumulation of antibacterial drugs in the human body will also produce a series of toxic effects.
Antibacterial drugs are not only directly abused in medical treatment. In recent years, with the rapid growth of global food trade and changes in food processing methods, a variety of therapeutic antibacterial drugs are widely used as prevention, treatment of animal and plant diseases and their growth promoters. It is widely used in livestock, aquaculture and plant cultivation, resulting in residues in food through related channels in food processing, causing great harm to the human body
[0003] At present, many detection methods for antibacterial drugs have been developed at home and abroad, which can be mainly divided into: microbiological method, immunological method, liquid chromatography, liquid chromatography-mass spectrometry, time-of-flight mass spectrometry, etc., but these methods are expensive Time-consuming, high cost, and low sensitivity are not enough to meet international standards

Method used

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  • Method for detecting antibacterial drugs of furazolidone and furacilin through surface-enhanced raman spectroscopy
  • Method for detecting antibacterial drugs of furazolidone and furacilin through surface-enhanced raman spectroscopy
  • Method for detecting antibacterial drugs of furazolidone and furacilin through surface-enhanced raman spectroscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Use acetone to prepare furazolidone into solutions with concentrations of 1000, 750, 100, 50, 10, 5, and 1 ppm, mix them with gold colloid at a ratio of 1:10, and use 1M HNO 3 solution or 1M NaOH solution to adjust the pH to 1.0-6.0, and scan the Raman spectrum to obtain the surface-enhanced Raman spectrum of each concentration gradient solution; mix acetone and gold sol for Raman detection to obtain the background Raman spectrum ; The surface-enhanced Raman spectrum of furazolidone solution is compared with the conventional Raman spectrum of furazolidone standard after deducting the background Raman spectrum, and it is determined that the characteristic peak is 1602cm -1 , 1554cm -1 , 1456cm -1 , 1326cm -1 , 1242cm -1 , 1162cm -1 , 968cm -1 It is used for qualitative; and the concentration is used as the abscissa, and the characteristic peak is 1008cm -1 The peak height is the ordinate, and a standard curve is made for quantification; the linear equation of the s...

Embodiment 2

[0020] Use acetone to prepare nitrofurazone into solutions with concentrations of 1000, 750, 100, 50, 10, 5, and 1 ppm, mix them with gold colloid at a ratio of 1:10, and use 1M HNO 3 solution or 1M NaOH solution to adjust the pH to 1.0-6.0, and scan the Raman spectrum to obtain the surface-enhanced Raman spectrum of each concentration gradient solution; mix acetone and gold sol for Raman detection to obtain the background Raman spectrum ; The surface-enhanced Raman spectrum of the nitrofurazone solution is compared with the conventional Raman spectrum of the furazolidone standard after deducting the background Raman spectrum, and the characteristic peak is determined to be 1602cm -1 , 1556cm -1 , 1484cm -1 , 1388cm -1 , 1336cm -1 , 1008cm -1 , 968cm -1 It is used for qualitative; and the concentration is used as the abscissa, and the characteristic peak is 1162cm -1 Peak height is ordinate, and standard curve is made in order to quantify; The linear equation that obtain...

Embodiment 3

[0022] Accurately weigh 2g of compound feed sample, add 50ml of acetone, ultrasonically extract in a 65°C water bath for 15min, and centrifuge at 3500r / min for 10min. Take 5ml of the supernatant and concentrate it to near dryness by rotary evaporation, and dilute to 2ml with acetone for Raman detection of furazolidone. A total of three parallel samples were made, and the analysis results are shown in Table 1; it can be seen from the analysis that the relative standard deviation of the parallel samples is 2.453%.

[0023] Table 1 The detection results of furazolidone in compound feed samples

[0024]

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Abstract

The invention utilizes a surface-enhanced raman spectroscopy to detect antibacterial drugs of furazolidone and furacilin. A method which adopts potassium chloroaurate to reduce sodium citrate is used for preparing aurosol; an acetone liquid which contains the furazolidone or the furacilin is mixed with the aerosol, the pH is regulated to 1.0-6.0, and a laser raman spectrometer with a laser light source of 785 nanometers is adopted to scan, so that a raman spectroscopy is obtained; 1611, 1560, 1488, 1472, 1397, 1343, 1260, 1173, 1022, 977, 806 and 775 centimeters <-1> are used as qualitative characteristic peaks for identifying the furazolidone, and 1602, 1554, 1456, 1336, 1162 and 966 centimeters <-l> are used as the qualitative characteristic peaks for identifying the furacilin; and moreover, the peaks of 1611centimeters <-1> and 1602 centimeters <-1> are respectively used for quantitatively determining the furazolidone and the furacilin. The method is convenient, quick, accurate and low in cost, and can be popularized and used in the fields food and drug testing.

Description

technical field [0001] The invention relates to a qualitative and quantitative detection method for antibacterial drugs furazolidone and nitrofurazone, specifically refers to the detection of antibacterial drugs furazolidone and nitrofurazone by using a surface-enhanced Raman spectrum method, and belongs to the field of feed, food, and drug detection. Background technique [0002] In recent years, the abuse of antibacterial drugs has become a public health problem of global concern. The harm is mainly due to the enhancement of drug resistance in the human body, which leads to the failure of antibacterial drugs. At the same time, the accumulation of antibacterial drugs in the human body will also produce a series of toxic effects. . Antibacterial drugs are not only directly abused in medical treatment. In recent years, with the rapid growth of global food trade and changes in food processing methods, a variety of therapeutic antibacterial drugs are widely used as prevention, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
Inventor 谢云飞竺芯宇吉薇郇楠陈婷姚卫蓉汪何雅钱和
Owner JIANGNAN UNIV
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