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Biochip with constant volume and preparation method thereof

A technology of biochip and fixed capacity, which is applied in biological testing, gaseous chemical plating, material inspection products, etc., and can solve the problems of inaccurate biochip detection results and complicated preparation methods

Inactive Publication Date: 2013-01-16
NO 49 INST CHINESE ELECTRONICS SCI & TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention aims to solve the problems of inaccurate detection results and complex preparation methods of existing biochips, and provides a fixed-capacity biochip and its preparation method

Method used

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  • Biochip with constant volume and preparation method thereof
  • Biochip with constant volume and preparation method thereof
  • Biochip with constant volume and preparation method thereof

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specific Embodiment approach 1

[0014] Specific embodiment one: in this embodiment, a biochip with a fixed capacity is made of a porous silicon layer 1, an epoxy resin layer 2 and a glass substrate 3; wherein the porous silicon layer is made of a silicon dioxide layer 1 -2, silicon nitride layer 1-3 and single crystal silicon layer 1-1, silicon dioxide layer 1-2 is located between silicon nitride layer 1-3 and single crystal silicon layer 1-1, single crystal silicon layer 1-1 and the glass substrate 3 are bonded together through the epoxy resin layer 2; the hole on the silicon wafer layer 1 with holes is the reaction zone 4.

[0015] This embodiment utilizes the anisotropy of single crystal silicon in potassium hydroxide solution etching. The so-called anisotropy from the perspective of crystal structure means that the periodicity and density of atomic arrangement of crystals along different directions are not the same, which leads to different physical and chemical properties of crystals in different direct...

specific Embodiment approach 2

[0016] Embodiment 2: This embodiment is different from Embodiment 1 in that: the glass substrate 3 is a transparent glass sheet. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0017] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the reaction zones 4 are distributed in an array on the biochip. Others are the same as in the first or second embodiment.

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Abstract

A biochip with a constant volume and a preparation method thereof relate to a biochip and a preparation method thereof. The invention solves the problems of inaccurate detection results and complicated preparation methods for current biochips. The biochip with a constant volume is formed by a porous silicon wafer layer, an epoxy resin layer and a glass substrate. The preparation method comprises the following steps: firstly, preparing the porous silicon wafer layer; secondly, coating epoxy resin on the glass substrate by spin coating or spray coating to obtain the epoxy resin layer, putting the porous silicon wafer layer on the epoxy resin layer to obtain a primary biochip, wherein a monocrystalline silicon layer of the porous silicon wafer layer contacts the epoxy resin layer; thirdly, curing the primary biochip to obtain the biochip with a constant volume. The biochip of the invention can accurately detect the concentration of a target molecule in a biological sample, and the method of the invention is simple. The invention is applicable to the crossing field of biotechnology and micromachining.

Description

technical field [0001] The invention relates to a biological chip and a preparation method thereof. Background technique [0002] Biochip is an epoch-making micro-analysis technology developed in the mid-1990s. It arranges a large number of biomolecular probes on the reaction area of ​​a special carrier in a required manner, interacts with the sample to be tested under specific conditions, and detects the target molecule through a sophisticated scanning instrument. [0003] At present, the carrier materials for preparing chips include glass sheets, silicon sheets, metal sheets, and nylon films. Due to the advantages of low fluorescence background, relatively good mechanical strength, good chemical stability, and good light transmission, glass slides are commonly used carrier materials for biochips. Surface modification of the carrier is a necessary link in the production of biochips. The main method is to chemically treat or coat organic molecules to attach chemical groups...

Claims

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Application Information

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IPC IPC(8): G01N33/48C23C16/34C23C16/40C23C16/44
Inventor 金建东王明伟齐虹田雷王永刚刘智辉郑丽张岩
Owner NO 49 INST CHINESE ELECTRONICS SCI & TECH GRP
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