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Bioactive peptide prepared from shellfish processing byproducts and preparation method thereof
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A technology of biological activity and by-products, applied in the field of biochemistry, to achieve the effects of improved conversion rate and biological activity, easy industrial production, and cheap and easy-to-obtain raw materials
Inactive Publication Date: 2013-01-23
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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However, the remaining soup after boiling and the remaining mantle after taking out the flesh are mostly discarded directly. However, a large amount of high-quality protein is contained in the discarded soup and mantle.
Method used
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Embodiment 1
[0046] The present invention will be further described by taking the mantle tissue, a by-product produced during the processing of scallops, as an example below.
[0047] 1. First, thaw the frozen mantle tissue, wash it, crush it with a grinder and homogenize it into mince;
[0048] 2. Add deionized water of 1 times the wet weight of the mantle tissue, boil for 20 minutes, and then cool to room temperature; the samples are divided into two groups, the first group is added with enzyme, and the second group is not added with enzyme as a control;
[0049] 3. Adjust the pH value of the two groups of samples to 7.5, and add compound enzymes (including protease and peptidase) to the first group according to the wet weight of raw materials: enzyme = 1:8000 (W / W);
[0050] 4. React at 45°C for 12 hours. After the reaction, treat in a boiling water bath for 10 minutes to terminate the reaction;
[0051]5. Centrifuge the hydrolyzate at 20,000 g for 20 min, and freeze-dry the obtained s...
Embodiment 2
[0072] The present invention will be further described by taking the by-product-boiled soup produced in the mussel processing process as an example below.
[0073] 1. First, thaw the frozen mussel boiling soup, boil for 10 minutes, and cool to room temperature; the samples are divided into two groups, the first group is added with enzyme, and the second group is not added with enzyme as a control;
[0074] 2. Adjust the pH value of two groups of samples to 7.5, and the first group adds compound protease (comprising protease and peptidase) step by step according to the dry weight of raw materialsolid content: enzyme=1:8000 (W / W);
[0075] 3. Reaction at 45°C and 100rpm for 6h;
[0076] 4. After the reaction is over, treat it in a boiling water bath for 10 minutes to terminate the reaction;
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Abstract
The invention discloses a peptide which is prepared from shellfishprocessingsolid and liquid byproducts and has angiotensin transferase inhibition activity as well as anti-oxidant activity and a preparation method thereof. The shellfishprocessing byproducts are subjected to fractional hydrolysis by a complex enzymecatalysis technology, and the hydrolysate is separated and purified to obtain the peptide with different physiological activity functions. According to the invention, a kind of peptidehydrolysate is obtained, which has high angiotensin transferase inhibition activity as well as free radical clearing ability and strong reducing power; and the peptide can be widely applied to the products such as healthcare food, functional food, auxiliary pressure reducing medicines and the like, and has broad market prospects. The technology is simple and feasible and favorable for industrial production.
Description
technical field [0001] The invention relates to the technical field of biochemistry, and more specifically relates to a functional peptide with physiological activity derived from shellfish and a preparation method thereof. Background technique [0002] Hypertension is a common and frequently-occurring disease worldwide, which is extremely harmful to human health. Since the drug treatment of hypertension will cause many adverse reactions, the peptides with antihypertensive activity extracted from natural proteins have the advantages of high food safety and no side effects, and will become an important part of non-drug treatment of hypertension in the future. [0003] Angiotensin I-converting Enzyme (ACE, EC 3.4.15.1) is an enzyme that acts on angiotensin I, which is produced by digesting angiotensinogen with renin , and converts it to angiotensin II by releasing two amino acids at its C-terminus. The action of angiotensin transferase can not only produce angiotensin II, wh...
Claims
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Patent Type & Authority Applications(China)
IPC IPC(8): C07K2/00C07K1/20C07K1/16C12P21/06
Inventor 孙媛霞管于平曾艳
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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