Method for quickly determining total amount of Vibrio parahaemolyticus in aquatic product

Abstract

The invention relates to a method for quickly determining total amount of Vibrio parahaemolyticus in an aquatic product, which comprises the following steps: (1) detection sample preparation; (2) double-layer flat plate preparation; (3) sample dilution; (4) sample detection; and (5) bacterial culture. The invention has the advantages of high selectivity, favorable test repetitiveness, accurate and reliable result, simple operation steps and low cost, can achieve the goal of quick detection, can read the result within 1-2d, does not need expensive or complex test materials, can prevent the counting result of the Vibrio parahaemolyticus from being overlow, is suitable for quantitative monitoring of Vibrio parahaemolyticus in the aquatic product, and has incomparable advantages and wide application prospects in the field of aquatic product quality safety.

Description

technical field [0001] The invention belongs to the field of quality and safety detection of aquatic products, in particular to a method for quickly measuring the total amount of vibrio parahaemolyticus in aquatic products. Background technique [0002] Vibrio parahaemolyticus (V.p) is a halophilic bacterium widely distributed in seawater, seabed sediments, seafood and salted food, and it is also the primary pathogen causing foodborne diseases in my country. The most important characteristic of this bacterium is that it needs salt to grow, so it is frequently isolated from seafood or salted food. After people eat food contaminated by this bacteria, it is very easy to cause food-borne diseases such as acute stomach pain, abdominal pain, and vomiting as the main symptoms. In severe cases, dehydration, shock, and even death may occur. For this reason, the bacterium has been listed as one of the key monitoring indicators for pathogenic microorganisms in seafood in my country. ...

AI Technical Summary

Problems solved by technology

The commonly used V.p quantitative detection method in the world is the maximum probable number (MPN) method in the US FDA Bacterial Management Manual. In 2008, my country released it as a national standard (GB / T 4789.7-2008). This method has high accuracy and application Wide range and other characteristics, but the detection takes a long time and the experimental work is cumbersome. It usually takes 4-5 days to get the result, which cannot meet the current needs of rapid food safety detection.

In addition, there are also research reports on the determination of V.p by using molecular biology methods and microbial rapid detection reagent sheets, but most of these methods have the characteristics of false positive or false negative, and the cost of the experiment is expensive, the technical requirements are high, and some cannot fully reflect the detection of V.p. The true extent of the bacterial contamination in the sample

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