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One-step Ball Milling Assisted Organic Solvent Efficient Polyphenol Extraction Process

An organic solvent and extraction process technology, which is applied in the field of high-efficiency polyphenol extraction process with one-step ball milling assisted by organic solvents, can solve the problems of environmental pollution of grape skin pomace, high energy consumption and high production cost, and achieves strong ability to eliminate free radicals, Simple process flow and strong antioxidant effect

Inactive Publication Date: 2015-09-30
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] [Purpose of the invention] The purpose of the present invention is to provide a one-step ball milling assisted organic solvent high-efficiency grape polyphenol extraction process, to solve the problems of environmental pollution caused by grape pomace in the wine industry chain and the difficulties faced in the high-value conversion process, and to use it Used in the production of high value-added medicines and health care products, etc., pointing out a new direction for the comprehensive utilization of fruit processing industry residues represented by grape pomace
[0005] [Concept of the present invention] The existing polyphenol extraction methods are either high in production cost, easy to cause environmental pollution, high in equipment investment cost, large in energy consumption, or serious in the absence of polyphenol activity, and cannot be well extracted. Promoted application of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Extraction of polyphenols from grape pomace

[0022] Crush grape pomace into uniform particles, then mix it with ethanol and zirconia grinding balls in a certain ratio (mass (g) / volume (ml) / number) 1:10:40, place in In a ball mill tank made of zirconia, after the ball mill is rotated at 1000rpm for 30 minutes, the supernatant is collected by centrifugation, concentrated under reduced pressure to obtain a crude product, and the extracted ethanol is recycled for reuse. Using the Prussian blue method and using catechol as the standard, the content of grape polyphenols measured was 37.70% higher than that of grape pomace after leaching in a water bath at 70°C for 30 minutes. After removing DPPH free radicals, OH- free radicals, O 2- Anti-oxidation experiments such as etc. show that the obtained polyphenols have a significant scavenging effect on free radicals, and the anti-oxidation effect is remarkable.

Embodiment 2

[0024] Extraction of polyphenols from apple pomace

[0025] Crush apple pomace into uniform particles, and then grind it with ethyl acetate and polytetrafluoroethylene balls in a certain ratio (mass (g) / volume (ml) / number) of 1:5:10, Put it in a ball mill jar made of polytetrafluoroethylene, rotate the ball mill at 100rpm for 60min, centrifuge to get the supernatant, concentrate under reduced pressure to obtain a crude product, and recover the extracted ethyl acetate for reuse. Using the Prussian blue method, with catechol as the standard, the measured polyphenol content was 30.20% higher than that of apple pomace directly extracted in water at 70°C for 30 minutes. After removing DPPH free radicals, OH- free radicals, O 2- Anti-oxidation experiments such as etc. show that the obtained polyphenols have a significant scavenging effect on free radicals, and the anti-oxidation effect is remarkable.

Embodiment 3

[0027] Extraction of Polyphenols from Pomegranate Peel

[0028] Crush the pomegranate pomace into uniform particles, and then place it in the agate material at a certain ratio (mass (g) / volume (ml) / number) of 1:100:100 with the grinding balls made of acetone and agate. In the ball mill tank, after the ball mill rotated at 1500rpm for 10min, the supernatant was centrifuged and concentrated under reduced pressure to obtain a crude product, and the extracted acetone was recovered and reused. Using the Prussian blue method, with catechol as the standard, the measured polyphenol content was 25.45% higher than that of pomegranate pomegranate pomace dregs directly extracted in water at 70°C for 30 minutes. After removing DPPH free radicals, OH- free radicals, O 2- Anti-oxidation experiments such as etc. show that the obtained polyphenols have a significant scavenging effect on free radicals, and the anti-oxidation effect is remarkable.

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Abstract

The invention discloses an efficient polyphenol extraction technology using a one-step ball milling assisted organic solvent mode. Extraction of polyphenol is finished in one step in the mode of extracting organic wastes rich in the polyphenol such as grape skin residue by using a polar organic solvent such as ethanol with the aid of ball milling; and a crude polyphenol product with high activity is obtained by performing concentration under reduced pressure, and the purified product can be added to products of medicine, health care and the like. The technology is simple in flow, strong in operability and wide in application, and can be applied to the extraction of the polyphenol in plants rich in the polyphenol such as grape, tea leaves, apple and pomegranate; and the extraction rate and the purity of the polyphenol are greatly improved, high-value utilization of the organic wastes such as the grape skin residue is promoted, and environmental pollution and resource waste are avoided at the same time.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a high-efficiency polyphenol extraction process with the aid of one-step ball milling for organic solvents. Background technique [0002] The increasing annual production and consumption of wine has promoted the rapid development of the wine industry. Grape pomace, as the leftovers in the wine processing process, has always been discarded in vain or dried and used as fuel. This practice not only pollutes the environment , and a waste of resources. Grape pomace is rich in polyphenols, which have strong antioxidant activity and the ability to scavenge free radicals. In addition, grape polyphenols also have the functions of anti-tumor, anti-arteriosclerosis, prevention of high blood pressure and skin care. Therefore, grape polyphenols have broad application prospects, especially in the fields of medicine and health products. If grape pomace can be comprehensively u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C37/70A61K36/87A61K36/82A61K36/73A61K36/185A23L1/29B01D11/02A61P39/06A61K131/00A23L33/00
Inventor 徐建彭华栋高婧
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI