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Porcine beta defensin-2 spray-dried powder, feed additive, premix and batch

A technology of β-defensin and spray-dried powder, applied in animal feed, animal feed, application, etc., can solve the problems of treatment failure, human health hazards, etc., and achieve the effects of reducing diarrhea rate, increasing feed intake, and simple equipment

Active Publication Date: 2013-02-06
宿迁大北农饲料有限责任公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, antibiotics have been widely used in livestock products, and their residues may cause animal pathogens to develop drug resistance. Drug-resistant genes can spread between bacteria through transformation, transduction, conjugation, translocation, etc., and may also pass through food chains and other ways Diffuse drug-resistant genes, so that bacterial drug-resistant genes can be passed between bacteria in the human population, bacteria in fauna, and bacteria in the ecosystem, which will lead to the increase of drug resistance of pathogenic bacteria and cause the treatment of infectious diseases in humans and animals Failure to cause harm to human health

Method used

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  • Porcine beta defensin-2 spray-dried powder, feed additive, premix and batch
  • Porcine beta defensin-2 spray-dried powder, feed additive, premix and batch
  • Porcine beta defensin-2 spray-dried powder, feed additive, premix and batch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of Pichia pastoris protein polypeptide porcine beta defensin 2 with antibacterial activity

[0022] 1. Preparation of Pichia pastoris fermentation broth

[0023] 1) Plate culture rejuvenation: Pichia pastoris carrying porcine β-defensin 2 gene (according to Jiemin et al., Expression and identification of recombinant porcine β-defensin 2 in Pichia pastoris, 2010, Chinese Journal of Veterinary Medicine, 20(8 ), prepared by the method disclosed in 1028-1031) strains were inoculated on YPD plate medium, and cultured at 28°C for 48h to rejuvenate Pichia pastoris and form a single colony. Cultivate for 48 hours;

[0024] 2) Preparation of primary seeds: Transfer the Pichia strain cultivated in step 1) to a 2L shaker flask filled with 300ml of YPD liquid medium, and culture on a shaker at 28°C for 48 hours until the late logarithmic period, to obtain primary seeds;

[0025] 3) Preparation of secondary seeds: Transfer the primary seeds prepared in step 2...

Embodiment 2

[0034] Example 2 Pichia pastoris protein polypeptide porcine beta defensin 2 gastric acid resistance test

[0035] 1. Comparison of antibacterial activity between ultrafiltrate and spray-dried powder

[0036] 1) Capsule uncapsulation: Weigh 0.5g of spray-dried bacteria powder and add it to 49.5mL of 0.05mol / L sodium citrate solution for uncapsulation, shake at 37°C and 200rpm for 12h, centrifuge at 3000rpm, and take the supernatant.

[0037] 2) Make an indicator plate: use the Oxford cup quantitative diffusion method, take Staphylococcus aureus as the indicator bacterium, and contain 10 indicator bacteria 7 Mix 1 ml of culture per ml with 25 ml of detection medium that has been melted and cooled to 50°C, pour it into the plate where the Oxford cup has been placed, and take out the Oxford cup with sterile tweezers after condensation.

[0038] 3) Comparison of antibacterial activity: Add 50 μL each of the ultrafiltrate and spray-dried powder solution into small wells, place in ...

Embodiment 3

[0042] Example 3 Effect of Pichia pastoris protein polypeptide porcine beta defensin 2 on growth performance of piglets

[0043] In the experiment, 120 Du×Chang×Da Sanyuan piglets aged 21 days were randomly distributed into 3 treatment groups and 1 blank control group, and each treatment had 3 repetitions. The Pichia pastoris protein polypeptide porcine beta defensin 2 spray dry powder prepared in Example 1 was added to the rations of each group, and the addition amounts were 0.5%, 1% and 2%, respectively. The measured growth performance indicators include: average daily gain (ADG), daily feed intake (ADFI), feed conversion efficiency (F / G), and observe the state of feces to calculate the diarrhea rate. The results are shown in Table 1.

[0044] Table 1 Effect of Pichia pastoris protein polypeptide porcine β-defensin 2 on piglet performance

[0045]

[0046] Note: There is no significant difference between peer data without shoulder label or with the same shoulder label l...

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Abstract

The invention belongs to the field of animal feed and particularly relates to porcine beta defensin-2 spray-dried powder, feed additive containing the porcine beta defensin-2 spray-dried powder, premix and batch. A preparation method of the porcine beta defensin-2 spray-dried powder includes fermenting bacterial strain, which carries porcine beta defensin-2 gene, of yeast genetic engineering, taking supernatant for ultrafiltration, sequentially adding protective agent, wall material and carrier to ultrafiltrate, and performing spray drying. The porcine beta defensin-2 spray-dried powder expressed by Pichia pastoris having antibacterial activity, and activity of porcine beta defensin-2 can be stored to a maximum extent by combining a spray-drying method and an encystation technology. In addition, the porcine beta defensin-2 is not damaged after invitro-simulation of artificial gastric juice, and released porcine beta defensin-2 has high activity.

Description

technical field [0001] The invention belongs to the field of animal feed, and in particular relates to a spray-dried porcine beta defensin 2 powder and feed additives, premixes and batches containing the same. Background technique [0002] In recent years, antibiotics have been widely used in livestock products, and their residues may cause animal pathogens to develop drug resistance. Drug-resistant genes can spread between bacteria through transformation, transduction, conjugation, translocation, etc., and may also pass through food chains and other ways Diffuse drug-resistant genes, so that bacterial drug-resistant genes can be passed between bacteria in the human population, bacteria in fauna, and bacteria in the ecosystem, which will lead to the increase of drug resistance of pathogenic bacteria and cause the treatment of infectious diseases in humans and animals Failure to do so will endanger human health. [0003] After entering the animal body, some antibiotics are f...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/00A23K1/16
Inventor 吴雅琨彭子欣陈慧鑫冯秋月谢飞王安如
Owner 宿迁大北农饲料有限责任公司
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