Actin binding peptide and purpose thereof

An actin and peptide-binding technology, applied in the field of medicine, can solve the problems of unsuitability for large-scale batch production, unsatisfactory treatment effect, high technical difficulty and high cost, and achieves the promotion of corneal wound healing and transparency recovery, and excellent repair effect. , the effect of synthesis difficulty and cost reduction

Active Publication Date: 2014-07-23
ZHAOKE GUANGZHOU OPTHALMIC DRUG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Tβ4 currently used in clinical research is a chemically synthesized product, which is technically difficult and expensive, and is not suitable for large-scale batch production, and the therapeutic effect is not ideal

Method used

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  • Actin binding peptide and purpose thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, the preparation of polypeptide LKKTETQ eye drops of the present invention

[0018] Using water as a solvent, sodium chloride and potassium chloride as an osmotic pressure regulator, calcium chloride and magnesium chloride as an electrolyte, and sodium hydroxide as a pH regulator, through a buffer solution containing sodium acetate and sodium citrate, the preparation containing the main drug LKKTETQ eye drops.

[0019] Weigh 6.4g of sodium chloride, 0.75g of potassium chloride, 0.48g of calcium chloride, 0.3g of magnesium chloride, 3.9g of sodium acetate, and 1.7g of sodium citrate into a container, add about 900ml of sterile water for injection, stir to dissolve ; Then add 0.02g actin-binding peptide (LKKTETQ peptide) and mix well, use 0.1mol / L sodium hydroxide solution to adjust the pH to 7.0, and finally add sterile water for injection to 1000ml. The solution was filtered through a sterile membrane, and filled with a sterile polypropylene bottle to obta...

Embodiment 2

[0020] Example 2, LKKTETQ repair test on rat corneal epithelium and matrix refractory damage after lamellar resection

[0021] Clean grade SD rats, 8-9 weeks old, male.

[0022] Rat half-thickness keratectomy model was made: rats were anesthetized by intraperitoneal injection of 1.5% pentobarbital sodium, the right eye was selected as the operated eye, and the corneal epithelium and about 1 / 2 of the depth of the corneal stroma were completely removed with a scalpel.

[0023] Eight lamellar excision model rats were randomly divided into 2 groups, 4 in each group, and LKKTETQ eye drops (specification: 1ml: 0.02mg) and recombinant human epidermal growth factor eye drops (trade name: Yibei, Produced by Guilin Huanuowei Gene Pharmaceutical Co., Ltd., 5ml / bottle), 1 drop / time (equivalent to about 0.03ml), administered 3 times a day at 8:00, 13:00, and 19:00. In the case of corneal perforation in the operated eye during the operation, or corneal ulcer or perforation in the observed ...

Embodiment 3

[0027] Embodiment 3, the research of LKKTETQ to the repairing effect of rabbit eye cornea

[0028] 20 white healthy rabbits, half male and half male, body weight 2±0.1kg / only, were kept in single cages during the experiment.

[0029] A circular wound surface with a diameter of 10mm was artificially created by corneal epidermis scraping and grinding, and the wound surface showed yellow-green fluorescence when examined by sodium fluorescein solution.

[0030] The animals were randomly divided into 5 groups, 4 in each group, half male and half male. The same group of animals was treated with the same concentration of LKKTETQ, and the experimental continuous treatment started 1 h after the wound formation. The right eyes of all animals were used as controls, 2 times a day, 1 drop of normal saline each time; the left eyes were repaired, 2 times a day, 1 drop of LKKTETQ solution each time, until the corneal wound was completely healed.

[0031] The change of corneal trauma area wa...

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Abstract

The invention relates to the field of biological medicine, particularly discloses actin binding peptide and the purpose of the actin binding peptide. The amino acid sequence of the actin binding peptide is LKKTETQ, the synthesis difficulty and the cost are lowered, the actin binding peptide is suitable for batch production in a large scale and has a brilliant preparing function on various corneal injuries, side effects do not appear in the dosing process, and corneal wound healing and transparency recovery are benefited. Compared with a curing method through thymosin beta4, the advantages are more obvious, the side effects are small, and the actin binding peptide can be used for corneal repairing treatment.

Description

technical field [0001] The invention relates to the field of medicine, in particular to an actin-binding peptide and its application. Background technique [0002] The cornea is the most densely distributed part of the human body, mainly distributed in the subepithelium and intraepithelium of the anterior 1P3 of the corneal stroma. The cornea not only has the general characteristics of epithelial and fibrous tissue in the body, but also has the characteristics of transparency, no blood vessels and rich sensory nerves. The natural healing of corneal injury is relatively slow. There are more chances of corneal surface damage, obvious discomfort symptoms, and increased chances of infection. Research on promoting superficial corneal wound healing is of great significance. [0003] After corneal injury, it mainly undergoes complex biological processes such as apoptosis, proliferation, migration and differentiation, decomposition, synthesis and deposition of extracellular matrix ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K38/08A61P27/02
Inventor 李小羿张国辉戴向荣凌娟胡代娣周冠群
Owner ZHAOKE GUANGZHOU OPTHALMIC DRUG
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