Industrial automatic production process for extracting casing heparin sodium

Abstract

The invention relates to an industrial automatic production process for extracting casing heparin sodium, belonging to the process for extracting the casing heparin sodium by adopting a salt dissolution method. The process provided by the invention comprises the following steps: (1) adding an intestinal mucosa solution into a salt dissolution tank, adding brine to adjust the baume degree to 3-5, adding alkaline water to adjust the pH value to 9.0-9.5, heating, and controlling the temperature of a mixed solution at 56-57 DEG C; (2) when the pH value of the solution is 8.3-8.4, adding an alkaline protease which is 1-1.5% of the total amount of the mixed solution to carrying out enzymolysis on the intestinal mucosa, preserving the heat of the solution at 56-57 DEG C for 2-3 hours; and (3) when the pH value of the solution is 7.6-7.8, heating the solution to 84-86 DEG C and preserving the heat for 10-20 minutes to finish the whole enzymolysis process. Due to the adoption of the process, the heparin and the casing protein can be separated from each other effectively, the cost is reduced, and the extraction amount and the purity of the heparin are improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a production process for extracting enteric-coated heparin sodium by salt solution. Background technique [0002] Heparin is a kind of mucopolysaccharide in mammals. It combines with protein and exists in intestinal mucosa, lung, liver and other organs. After heparin and protein are separated and extracted, it has various physiological functions such as anticoagulation, antithrombotic, and blood fat reduction. It is an effective drug for preventing atherosclerosis and cardiovascular and cerebrovascular diseases. Due to the development of food, chemical and pharmaceutical industries in recent years, the consumption of heparin sodium is also increasing year by year. [0003] Industrial production of heparin is a wet physical separation process of heparin and heparin protein. According to different separation methods, the common extraction methods of heparin sodium include salt hydrolysi...

AI Technical Summary

Problems solved by technology

[0003] Industrial production of heparin is a wet physical separation process of heparin and heparin protein. According to different separation methods, the common extraction methods of heparin sodium include salt hydrolysis, enzymatic hydrolysis, etc., but no matter which method is first, the intestinal mucosa is first extracted. Carry out salt hydrolysis treatment, the smaller the particle size is, the more the cell membrane can be broken, and the heparin will be released from the cells as much as possible. The enzymatic hydrolysis process is very important for the extraction of heparin, but the old process is directly added manually After the salt solution and alkaline solution are stirred, rely on pH test paper and thermometer to observe the changes of the data. Not only will there be errors in the data, but also a certain amount of labor costs will be added. The operation cannot reach the degree of complete automation. Adding too much salt will If the exchange adsorption content in the next step exceeds the specified level, too little salt is added, the separation of heparin and protein is not complete, and the alkalinity is too strong, the heparin will be degraded and destroyed, and the yield will decrease.

At the same time, it can also increase the solubility of some proteins, making filtration difficult

Insufficient alkalinity causes the extract to be acidic, and under high temperature, the heparin will be destroyed rapidly. If the temperature rises too quickly, the protein will coagulate early, which will affect the decomposition and dissolution of heparin.

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