Beauveria bassiana capable of catalyzing asymmetric reduction reaction and application thereof

Abstract

The invention discloses beauveria bassiana capable of catalyzing an asymmetric reduction reaction, the bacterial strain is preserved in a China microorganism culture preservation management council common microorganism center, a preservation number is CGMCC NO.6345, and preservation time is July 12th, 2012. The bacterial strain can express carbonyl reductase high in stereoselectivity. When the bacterial strain is used for conducting whole-cell catalysis of 4-ethyl acetoacetate, enantiomer excess of S-4-chlorine-ethyl-3-hydroxybutyrate in products is 100% e.e.; and when the bacterial strain is used for conducting whole-cell catalysis of N,N-dimethyl-3-oxo-3-(2-thiophene)-1-propylamine hydrochloride, enantiomer excess of (S)-N,N-dimethyl-3-hydroxy-3-(2-thiophene)-1- propylamine hydrochloride is 100% e.e..

Description

technical field [0001] The invention relates to a fungus, in particular to a Beauveria bassiana which can catalyze an asymmetric reduction reaction and application thereof. Background technique [0002] 4-Chloro-3-hydroxybutyric acid ethyl ester contains active chlorine atom, which makes it widely used in some asymmetric synthesis and becomes an important chiral intermediate of many chiral drugs. S-4-Chloro-3-hydroxybutyrate ethyl ester (S-CHBE) is a key chiral intermediate in the synthesis of Slagenins B and C, HMG-CoA reductase inhibitors, which can also be reduced to 1,4-dihydro Pyridine-type beta blockers. Ethyl R-4-chloro-3-hydroxybutyrate can be used as a key chiral intermediate for the synthesis of L-carnitine, R-γ-amino-β-hydroxybutyric acid, (-)-large lactam A, And can be transformed into (+)-negative mycin. [0003] Duloxetine, the chemical name is (+)-S-N-methyl-γ-yl-(1-naphthyloxy)-2-thienyl hydrochloride, which is the active ingredient in Cymbalta. It is a 5 ...

AI Technical Summary

Problems solved by technology

[0009] The invention provides a Beauveria bassiana that can catalyze an asymmetric reduction reaction. The strain can express a carbonyl reductase with high stereoselectivity, which solves the problem that the traditional wild strains cannot be reduced to obtain a single optically pure compound and the optical purity is low.