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Heavy metal-resistant polycyclic aromatic hydrocarbon (PAHs) degrading bacteria and application thereof in remediation of composite contaminated soil

A technology for polycyclic aromatic hydrocarbons and heavy metals, applied in the fields of environmental engineering and microbial engineering, can solve the problems of lack of polycyclic aromatic hydrocarbon degrading strains, etc., and achieve the effects of wide range of degradation substrates, broad application potential and rapid degradation ability

Active Publication Date: 2013-02-27
北京霖孚科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the prior art lacks a polycyclic aromatic hydrocarbon degrading strain that can tolerate a heavy metal environment

Method used

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  • Heavy metal-resistant polycyclic aromatic hydrocarbon (PAHs) degrading bacteria and application thereof in remediation of composite contaminated soil
  • Heavy metal-resistant polycyclic aromatic hydrocarbon (PAHs) degrading bacteria and application thereof in remediation of composite contaminated soil
  • Heavy metal-resistant polycyclic aromatic hydrocarbon (PAHs) degrading bacteria and application thereof in remediation of composite contaminated soil

Examples

Experimental program
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Effect test

Embodiment 1

[0044] The separation and screening method of tzyx1 provided by the invention.

[0045] Collect surface soil samples 5-20cm below the surface from the solid waste dismantling site, and use them as bacteria sources for natural enrichment culture. Glucose 2g, tryptone 4g, beef extract 4g, MgSO 40.8g was added to 400mL of distilled water, the prepared culture solution was sterilized at 121°C for 20 minutes, cultivated on this medium for three days, and then prepared inorganic salt basic medium, adding pyrene, benzanthracene, The polycyclic aromatic hydrocarbon compound of benzopyrene, then add heavy metal ions, after being prepared into a solution, then add distilled water and settle to 500mL. , benzoanthracene, and benzopyrene have a final concentration of 200 mg / mL, and the above-mentioned addition of heavy metal Cu 2+ , Zn 2+ , Pb 3+ The amount should make the heavy metal Cu in the prepared culture solution 2+ , Zn 2+ , Pb 3+ The final concentration of each was 200 mg / mL...

Embodiment 2

[0048] Tzyx1 degrades polycyclic aromatic hydrocarbons in complex-contaminated soils.

[0049] Select 100g of soil polluted by heavy metals and polycyclic aromatic hydrocarbons, mix tzyx11 with the soil at an amount of 2.0% (mass percentage), add a small amount of water to keep the soil moisture content at about 15%, put it into a constant temperature oscillator, and control the cultivation temperature at 25 Under the condition of constant temperature of ℃, shake culture and remediate for 15 days, the remediation of complex polluted soil can be realized, and the remediated soil can be obtained.

[0050] Figure 1-Figure 6 The kinetic degradation curves of PAHs naphthalene, phenanthrene, fluoranthene, pyrene, benzanthracene, and benzopyrene over time in different treatment soils were respectively shown. The three different treatments were PAH-contaminated soil samples (CK), without heavy metals and inoculation; PAH-contaminated soil samples without heavy metals, inoculated wit...

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Abstract

The present invention belongs to the technical field of environmental engineering and microbial engineering, and especially relates to klebsiella pneumoniae tzyx1 for degradation of PAHs and application thereof in remediation of composite contaminated soil. The accession number of klebsiella pneumoniae tzyx1 strain in the center of the Chinese Typical Culture Collection Center is CCTCC M2012239. The klebsiella pneumoniae tzyx1 strain has the ability to degrade PAHs. The strain can be applied in the governance of PAHs and heavy metal-contaminated soils.

Description

technical field [0001] The invention belongs to the technical fields of environmental engineering and microbial engineering. In particular, it relates to a strain of Klebsiella pneumoniae tzyx1 that degrades polycyclic aromatic hydrocarbons and its application in remediation of complex contaminated soil. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of persistent organic pollutants (POPs). The source of PAHs pollution mainly comes from waste machine oil, lubricating oil of electrical products, and hydrocarbon-containing substances such as wood, organic polymer compounds, paper, etc. produced by thermal decomposition. Because PAHs and their derivatives have carcinogenicity, mutagenicity and teratogenicity, as well as acute toxicity and sublethal effects, they have attracted great attention. [0003] The remediation of polycyclic aromatic hydrocarbons (PAHs) contaminated soil is a research hotspot in current environmental remediation. Bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/22
Inventor 奚立民张昕欣于红艳
Owner 北京霖孚科技集团有限公司
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