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Novel microalgal food compositions

A composition and food technology, applied in food science, food ingredients, food preservation, etc., can solve problems such as instability and oxidation of DHA

Inactive Publication Date: 2013-02-27
CORBION BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, DHA is not suitable for cooked food, because it will be oxidized by heat treatment
Additionally, DHA is unstable when exposed to oxygen even at room temperature and in the presence of antioxidants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0359] Cultivate microalgae for high oil content

[0360] The microalgal strains were grown in shake flasks with the goal of obtaining more than 20% oil by dry cell weight. The medium in the shake flask used was as follows: K 2 HPO 4 : 4.2g / L, NaH 2 PO 4 : 3.1g / L, MgSO 4 ·7H 2 O: 0.24g / L, citric acid monohydrate: 0.25g / L, CaCl 2 2H 2 O: 0.025g / L, yeast extract: 2g / L and 2% glucose. The frozen cells were thawed at room temperature, 500ul of cells were added to 4.5ml of medium, and grown on a 6-well plate at 28°C for 7 days while stirring (200rpm). Dry cell weight was determined by centrifuging 1 ml of the culture at 14,000 rpm for 5 minutes in pre-weighed Eppendorf tubes. The culture supernatant was discarded, and the resulting cell pellet was washed with 1 ml of deionized water. The culture was centrifuged again, the supernatant was discarded, and the cell pellet was placed at -80°C until frozen. Samples were then freeze-dried for 24 hours and the dry cell weight ...

example 2

[0367] To generate algal biomass with high oil content, three fermentations were performed with three different media formulations. The first formulation (medium 1) is based on the medium described by Wu et al. (1994 Science in China, volume 37, phase 3, pages 326-335), and the composition per liter is: KH 2 PO 4 , 0.7g; K 2 HPO 4 , 0.3g; MgSO 4 -7H 2 O, 0.3g; FeSO 4 -7H 2 O, 3mg; Thiamine hydrochloride, 10μg; Glucose, 20g; Glycine, 0.1g; H 3 BO 3 , 2.9 mg; MnCl 2 -4H 2 O, 1.8 mg; ZnSO 4 -7H 2 O, 220 μg; CuSO 4 -5H 2 O, 80 μg; and NaMoO 4 -2H 2 O, 22.9 mg. The second substratum (medium 2) is derived from the substratum in the shake flask among the example 1, and the composition of every liter is: K 2 HPO 4 , 4.2g; NaH 2 PO 4 , 3.1g; MgSO 4 -7H 2 O, 0.24g; Citric Acid Monohydrate, 0.25g; Calcium Chloride Dehydrate, 25mg; Glucose, 20g; Yeast Extract, 2g. The third culture medium (medium 3) is a mixed culture medium, and the composition per liter is: K 2 H...

example 3

[0373] Preparation of biomass for food products

[0374] Microalgal biomass was produced by culturing microalgae as described in any of Examples 1-2. Harvest microalgal biomass from fermenters, flasks, or other bioreactors.

[0375] Comply with GMP procedures. Food, food If there is a clear possibility of contamination of contact surfaces or food packaging materials, he shall be prohibited from performing any operation expected to result in such contamination until the condition is corrected. Instruct personnel to report such medical conditions to their supervisor. All workers who come into direct contact with microalgal biomass, biomass contact surfaces, and biomass packaging materials are expected to observe hygienic practices while on duty to the extent necessary to protect the microalgal biomass from contamination. Methods of maintaining cleanliness include, but are not limited to: (1) Wearing garments suitable for handling in a manner that protects biomass, biomass ...

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Abstract

The invention provides novel microalgal food compositions comprising microalgal biomass that have been processed into flakes, powders and flours. The microalgal biomass of the invention is low in saturated fats, high in monounsaturated triglyceride oil and can be a good source of fiber. The invention also comprises microalgal biomass that is suitable as a vegetarian protein source and also as a good source of fiber. Novel methods of formulating food compositions with the microalgal biomass of the invention are also disclosed herein including beverages, baked goods, egg products, reduced fat foods and gluten-free foods. The provision of food compositions incorporating the microalgal biomass of the invention to ahuman have the further benefit of providing healthful ingredients while achieving levels of satiety sufficient to reduce further caloric intake. The invention also provides novel strains of microalgae that have been subject to non-transgenic methods of mutation sufficient to reduce the coloration of the biomass produced by the strains. Oil from the microalgal biomass can be extracted and is an edible oil that is heart-healthy. The novel microalgal biomass and oil therefrom can be manufactured from edible and inedible heterotrophic fermentation feedstocks, including corn starch, sugar cane, glycerol, and depolymerized cellulose that are purpose-grown or byproducts of existing agricultural processes from an extremely broad diversity of geographic regions.

Description

[0001] Cross References to Related Applications [0002] This application is required under 35 U.S.C. 119(e) of U.S. Provisional Patent Application No. 61 / 169,271 filed April 14, 2009, U.S. Provisional Patent Application No. 61 / 173,166 filed April 27, 2009, and Sep. 25, 2009 U.S. Provisional Patent Application No. 61 / 246,070, filed January 28, 2010, and U.S. Provisional Patent Application No. 61 / 299,250, filed January 28, 2010. This application is also a continuation-in-part of International Application No. PCT / US2009 / 060692, filed October 14, 2009, as required by 35 U.S.C. 119(e) of U.S. Provisional Patent Application No. 61 / , filed April 27, 2009 173,166, the benefit of U.S. Provisional Patent Application No. 61 / 246,070, filed September 25, 2009. This application is also U.S. Application Nos. 12 / 684,884, 12 / 684,885, 12 / 684,886, 12 / 684,887, 12 / 684,888, 12 / 684,889, 12 / 684,891, 12 / 684,892, 12 / 684,893, filed January 8, 2010 and a continuation-in-part of 12 / 684,894, and each of w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B5/00A23L7/10A23L7/109A23L13/40A23L15/00A23L17/60A23L23/00A23L27/60
CPCA23V2002/00A23L17/60A23L7/10A23V2250/202A23V2250/5488A23V2250/5424A23V2250/70A23V2250/1868A23V2250/2136A23V2250/1882A23V2250/712A23V2250/628A23V2250/5114A23V2250/1842A23L33/17A23L7/126A23L13/424A23L27/60A23L33/115A23L7/13A23L33/21A23L23/00A23L15/30A23L2/52
Inventor G·布鲁克斯S·富兰克林J·阿维拉S·M·戴克尔E·巴利乌W·莱基特斯基J·皮朔基D·兹达尼斯L·M·诺里斯
Owner CORBION BIOTECH INC
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