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Use of vanillic acid derivatives in the treatment of systemic autoimmune diseases

An autoimmune, vanillic acid technology, applied in nervous system diseases, muscular system diseases, neuromuscular system diseases, etc., can solve problems such as other biological activities that have not been reported, reduce deposition and kidney tissue damage, and inhibit delayed Sexual hypersensitivity, the effect of reducing antibody levels

Active Publication Date: 2016-09-14
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Vanillic acid glucoside has been isolated from plants such as Prunus japonica, but other biological activities have not been reported except antioxidant activity

Method used

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  • Use of vanillic acid derivatives in the treatment of systemic autoimmune diseases
  • Use of vanillic acid derivatives in the treatment of systemic autoimmune diseases
  • Use of vanillic acid derivatives in the treatment of systemic autoimmune diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Vanillic acid glucoside has obvious inhibitory effect on the proliferation of mouse spleen T and B lymphocytes cultured in vitro

[0027] 1.1 Materials and methods

[0028] Clean-grade BALB / c mice, male, weighing (19±2) g, were purchased from the Experimental Animal Center of the Chinese Academy of Medical Sciences. LPS, ConA, DMSO, MTT Sigma products. RPMI 1640 complete medium contains penicillin 100U / L, streptomycin 100U / L, glutamine 2mM and 10% fetal bovine serum. The mice were killed by neck dislocation, and the spleen was taken aseptically, and the lymphocyte suspension was prepared according to the routine, and the cells were adjusted to 8×10 cells with RPMI1640 complete medium. 9 / L, 0.1mL / well to inoculate a 96-well plate. Add ConA (final concentration is 2.5mg / L) or LPS (final concentration is 10mg / L), and vanillic acid glucoside (final concentration is 1X10 -9 -1X10 -5 mol / L), 6 replicate wells for each concentration. 37°C, 5% CO 2 cultured i...

Embodiment 2

[0038] Example 2. Vanillic acid glucoside has obvious inhibitory effect on delayed hypersensitivity in mice

[0039] 2.1 Materials and methods

[0040] Balb / c mice, male, 18-20 g, were purchased from the Experimental Animal Center of the Chinese Academy of Medical Sciences. They were randomly divided into three groups, and the experiment started after one week of adaptive feeding. Sensitized mBSA (Sigma) was added with normal saline for injection to 5 mg / mL, and fully emulsified with an equal volume of complete Freund's adjuvant (CFA, Sigma). Except for the normal control group, 100 μl of emulsifier was intradermally injected into the abdomen of each mouse. From the 5th day of drug sensitization, intragastric administration was carried out daily, 0.2ml / bird, for 3 consecutive times. The normal and model control groups were given distilled water, 4 dose groups of test drug vanillic acid glucoside 1.1, 3.3, 10, and 20 mg / Kg body weight, and 2 dose groups of positive drug indo...

Embodiment 3

[0047] Example 3. The improvement effect of vanillic acid glucoside on active DNA-induced systemic lupus erythematosus syndrome-like mouse antibody level and renal tissue damage

[0048] 3.1 Materials and methods

[0049] Activation of splenic lymphocytes and extraction of genomic DNA BALB / c mice were sacrificed, spleens were taken aseptically, spleen cell suspensions were routinely prepared, counted with trypan blue (survival rate > 95%), adjusted with RPMI 1640-20% fetal bovine serum Cell 2X10 9 / L, add Con A to make the final concentration 3mg / L, add 20mL per bottle into a culture bottle, culture upright in a 5% CO2 incubator at 37°C, take it out after 48h, and collect activated cells by centrifugation. Active DNA was prepared according to the operation steps of the kit by using a large number of rapid extraction kits for animal genome DNA (Beijing Biotech Biogene Technology Co., Ltd.). Establishment of systemic lupus erythematosus syndrome-like mouse model BALB / c mice, 6...

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Abstract

The invention discloses the application of the vanillic acid derivatives represented by the formula (I) in the preparation of medicines for treating systemic autoimmune diseases. Preferred systemic autoimmune diseases are selected from systemic lupus erythematosus, rheumatoid arthritis, systemic sclerosis, Sjogren syndrome, polymyositis.

Description

technical field [0001] The invention relates to a new application of vanillic acid glucoside in treating autoimmune diseases such as systemic lupus erythematosus. Background technique [0002] Autoimmune diseases refer to the body's immune response to its own components to produce autoantibodies and / or effector T lymphocytes targeting self-antigens, resulting in damage and dysfunction of its own tissues or cells. Systemic autoimmune diseases, also known as systemic autoimmune diseases, include systemic lupus erythematosus (Systemic Lupus Erythematosus, SLE), rheumatoid arthritis (RA), systemic Sclerosis (SSc), Sjogren's Syndrome (SS), etc. Its basic feature is that the patient's blood is positive for antinuclear antibodies, and the use of immunosuppressant therapy has a certain effect. [0003] Take the more serious systemic lupus erythematosus as an example. SLE is an autoimmune disease that can affect the skin and multiple systems of the body, and the exact etiology is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7034A61P37/00A61P29/00A61P19/02A61P25/00A61P27/02A61P1/02A61P21/00
CPCA61K31/7028A61K31/7034A61P1/02A61P19/02A61P21/00A61P25/00A61P27/02A61P29/00A61P37/00A61P37/02
Inventor 殷红董俊兴
Owner INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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