Hydrogel for three-dimensional culturing of tumor cells and applications thereof

Abstract

The invention discloses hydrogel for three-dimensional culturing of tumor cells and applications thereof, and particularly relates to applications in fundamental research related to oncology and anti-tumor drug screening. The hydrolysis product of polymethyl vinyl ether maleic anhydride, i.e., methyl vinyl ether maleic anhydride copolymer, and a hydrogel cross-linking agent polyethylene glycol are used for a cross linking reaction to prepare the hydrogel suitable for being used as a three-dimensional culture medium of various tumor cells. Compared with commercialized BME gel and Collagenl gel available on the market, the hydrogel is determined to have the capabilities of simulating in-vitro environment and supporting three-dimensional growth of growth of cells in the culture medium through researching diversity factors of cell adhesion abilities and cell growth curves on tumor cell transfer and invasion, drug resistance experiments of anti-tumor drugs and the like, thus providing a three-dimensional cell culture model for researches on tumor molecular biology characteristics, drug screening and the like.

Description

technical field [0001] The invention relates to the field of preparation of medical materials, in particular to the application of a hydrogel applicable to the three-dimensional culture of tumor cells in the three-dimensional culture of tumor cells and the establishment of a high-throughput screening model for anti-tumor drugs. Background technique [0002] Tissues in the human body are three-dimensional (3D) structures, but when studying the structure, function, and pathology of human tissues, they often rely on in vitro two-dimensional (2D) cultures and animal models. The use of ordinary 2D cell culture to study the mechanism of tumor development is very different from the real in vivo environment, such as cell-cell, cell-matrix interaction, cell differentiation, etc. (See Nelson C.M., Mina J. Bissell. Of extracellular matrix , scaffolds, and signaling: tissue architecture regulates development, homeostasis, and cancer [J]. Annu. Rev. Cell Dev. Biol, 2006, 22: 287–309.). ...

AI Technical Summary

Problems solved by technology

The 3D culture gene can simulate the tissue center necrosis and the interaction microenvironment with the extracellular matrix caused by the lack of oxygen and nutrition in the tumor tissue in vivo, so as to objectively reflect the phenomenon of tumor tissue induced angiogenesis and drug resistance to chemotherapeutic drugs , while ordinary 2D culture cannot be simulated (see Kevin O. Hicks, Frederik B. Pruijn, Timothy W. Secomb et al. Use of three-dimensional tissue cultures to model extravascular transport and predict in vivo activity of hypoxia-targeted anticancer drugs [J ]. J. Natl. Cancer Inst, 2006, 98: 1118–1128.)

Because animal gel contains many uncertain components and the component differences between batches affect the accuracy and objectivity of drug evaluation results; on the other hand, animal gel is expensive, which limits its wide application. application

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