Magnetic separation-based multi-sample multi-site high-flux nucleic acid analysis system

Abstract

The invention discloses a magnetic separation-based multi-sample multi-site high-flux nucleic acid analysis system which comprises four parts, namely an XYZ three-axis mechanical arm and control circuit, an accurate temperature control system worktable and control circuit, a high-flux fluorescence signal detection worktable and control circuit and system control software. According to the system, a porous plate serves as a reaction container, various function modules are contained to be matched with the system, various biochemical reactions in the nucleic acid detection process can be finished under coordination and control of the system control software, and the specific functions comprise accurate liquid transfer, accurate working plate transfer, rapid magnetic separation, automatic sucker installation and discard, accurate temperature control process, low-temperature sample storage, high-flux fluorescence signal detection and system software overall control. According to the magnetic separation-based multi-sample multi-site high-flux nucleic acid analysis system, a magnetic separation technology, a particle microarray technology, a single base extension technology and an automation system are combined, and a novel high-flux nucleic acid detection system is established on the basis of a series of novel high-flux, automatic, high-specificity and high-practicality single base difference detection methods.

Description

1. Technical field [0001] The invention relates to a high-throughput nucleic acid analysis instrument used in biomedical research and clinical diagnosis, in particular to a multi-sample multi-site high-throughput nucleic acid analysis system based on magnetic separation 2. Background technology [0002] With the completion of the Human Genome Project (HGP), the research on human genetic information is shifting from the determination of DNA (Deoxyribonucleic Acid) sequence and the interpretation of genetic information to the biological function at the level of molecular biology. At the research level, by comparing the sequences of patients and normal people, those suspicious nucleic acid mutation sites can be found. These variable sites, or markers, the most common type is single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP), which has become the most commonly used third-generation genetic marker. SNP is suitable for genetic analysis of complex traits and res...

AI Technical Summary

Problems solved by technology

Faced with the SNP locus typing of a large number of samples, relying solely on the manual operation of the experimenter is not only difficult to meet the requirements in terms of speed, but also the experimenter will inevitably make mistakes when faced with high-intensity work, especially in gene amplification-based In terms of molecular diagnosis, any small error will generate false negative or false positive signals

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