Preparation method for Newcastle disease and avian influenza bi-combined inactivated vaccine

Abstract

The invention relates to a preparation method for Newcastle disease and avian influenza bi-combined inactivated vaccine. The preparation method comprises the following steps: inoculating, chick embryo post-incubation, concentration of virus liquid, inactivation and incubation of virus liquid, thereby obtaining the Newcastle disease and avian influenza bi-combined inactivated vaccine. According to the preparation method, a continuous syringe and a magnetic stirrer are matched for inoculation, so that the efficiency is improved, and the loss of virus seed titer can be reduced; milipore quick tangential flow concentration equipment is used for concentration, so that the concentration efficiency is improved, and the titer loss in the concentration process can be decreased; and beta-propiolactone adopted as inactivator has no residuals and hazard, and has no untoward effect after being used.

Description

technical field [0001] The invention relates to a method for preparing an inactivated vaccine for poultry, in particular to a method for preparing a dual inactivated vaccine for Newcastle disease and avian influenza. Background technique [0002] Newcastle disease, commonly known as chicken plague, is a highly contagious infectious disease caused by chicken Newcastle disease virus. It mainly infects the respiratory tract and digestive tract. Chickens of all ages can be infected, and young chickens are the most serious, with a morbidity and mortality rate of up to 80%. Above; Avian influenza is an infection and / or disease syndrome of birds caused by type A influenza virus. Infected house-fed poultry, including poultry, can exhibit clinical symptoms, respiratory disease, decreased egg production, acute hemorrhagic disease with high mortality, and subclinical symptoms, mild respiratory infections to asymptomatic Symptoms are poisonous and other popular forms. According to the...

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Problems solved by technology

[0005] 1) The BCG syringe is used for inoculation, and it is necessary to manually absorb the venom repeatedly for inoculation. Manually absorbing the venom is time-consuming and low in efficiency. The uniformity of the virus seed cannot be guaranteed. On the other hand, the potency of the seed poison will be affected in the environment of normal temperature for a long time, which often leads to a drop in potency, thereby reducing the potency of the produced antigen.

[0006] 2) Concentration using a filter column to concentrate, the amount of concentration at one time is small, the efficiency is low, time-consuming, and the concentration time is long to reduce the antigen titer and affect the concentration effect

[0007] 3) the deactivator that adopts is formaldehyde, and formaldehyde is carcinogen, has strong irritation, and when formaldehyde acts on virus capsid protein, easily makes protein cross-linking or virus particle aggregation, can no longer act on the nucleic acid in capsid protein, In this way, the antigenicity of the pathogen protein will be severely damaged, and the pathogen may survive, and there is a risk of incomplete inactivation. If the residual free formaldehyde in the vaccine is injected into the body with the vaccine, it is easy to produce an irritant reaction.

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