Preparation method and application of PDMS polymer chip of arc-shaped concave aperture

A technology of arc-shaped depressions and polymers, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve the problems of high price and complicated operation, and achieve the effect of simple operation and low experimental cost

Active Publication Date: 2013-03-20
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a preparation method and application of a PDMS polymer chip with arc-shap

Method used

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  • Preparation method and application of PDMS polymer chip of arc-shaped concave aperture
  • Preparation method and application of PDMS polymer chip of arc-shaped concave aperture
  • Preparation method and application of PDMS polymer chip of arc-shaped concave aperture

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Experimental program
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Effect test

Embodiment 1

[0031] The SU-8 polymer template is made by photolithography technology, the template is a 20×20 circular hole, and the structure of the template is cylindrical. like figure 1 As shown, apply the SU-8 photoresist on a clean glass slide, heat it at 95 degrees Celsius for 1 hour; put the designed mask on and expose it to ultraviolet light for 60 seconds; heat it at 95 degrees Celsius for 15 minutes, and develop it for 5 minutes. Heat curing at 80°C for 2 hours; full exposure for 90 seconds. Uncured PDMS (10:1) was poured over the SU-8 polymer template and heated at 80 °C for 20 min at the same time. After the device is cooled, the upper PDMS chip is peeled off to form a counter-mold. Pour the uncured PDMS (10:1) onto the PDMS polymer anti-mold chip and heat it at 80°C for 20 minutes at the same time. After the device cools down, the upper layer of PDMS polymer can be peeled off to form arc-shaped arc-shaped concave holes. PDMS polymer chip.

Embodiment 2

[0033] The principle and flow chart of the three-dimensional culture of rat chondrocytes on the PDMS polymer chip with curved concave holes figure 2 shown. The PDMS polymer chip with arc-shaped concave holes is first sterilized, irradiated with ultraviolet light overnight, and then modified with 1% PF-127 solution for 8 hours, inoculated with chondrocyte suspension, and the chondrocytes are under the action of gravity. It will settle to the bottom of the small well on the surface of the chip and spontaneously form a three-dimensional cell cluster.

Embodiment 3

[0035] Based on SU-8 as a template, the rapid fabrication of arc-shaped concave small-hole PDMS polymer chip was used for three-dimensional culture of rat chondrocytes, and the maintenance of subtype and function. image 3 It shows the growth of rat chondrocytes cultured on the PDMS polymer chip with arc-shaped concave holes for three days. The connection between cells is getting tighter and the tendency of clustering is obvious. This proves that the arc-shaped PDMS polymer chip with concave holes can form a three-dimensional cell structure. Figure 4It shows the immunofluorescence staining of type II collagen after the mouse chondrocytes were cultured on the PDMS polymer chip with arc-shaped concave holes for three days. The control group was two-dimensional rat chondrocytes cultured in the well plate. The expression of type Ⅱ collagen in rat chondrocytes was obvious. Figure 5 It shows the photo of immunofluorescent staining of proteoglycan after three days of culture of mo...

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Abstract

Provided are a preparation method and application of PDMS polymer chips of arc-shaped concave apertures. The method includes the steps of: making use of the soft etching technology to make a PDMS channel chip template; producing PDMS film, sealing the PDMS channel chip template with the PDMS film to seal with a glass plate, connecting the bottom with gas by a metal pipe, modifying the apparatus surface; pouring the uncured PDMS polymer solution into the apparatus, feeding the gas, heating to cure the PDMS polymer solution, and peeling the PDMS chip. The PDMS chip after surface modification can be used for three-dimensional culture of cells. The method does not require expensive etching equipment, has the advantages of simple and rapid operation, low experiment cost, no organic reagents, and environmental protection, and can be integrated with other technologies.

Description

technical field [0001] The invention relates to the processing, manufacture, modification and application fields of polymer chips, and in particular provides a preparation method and application of a PDMS polymer chip with arc-shaped concave holes. Background technique [0002] Tissue engineering is a new discipline emerging in recent years. It is the application of the principles and technologies of life science and engineering, on the basis of a correct understanding of the relationship between the structure and function of mammals in both normal and pathological states, research and development for the repair, maintenance, and promotion of various tissues or organs of the human body Functional and morphological biosurrogates after injury. The core of tissue engineering is to establish a three-dimensional complex of cells and biomaterials, that is, a living tissue with vitality, which is used to reconstruct the shape, structure and function of the diseased tissue and achi...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/077
Inventor 秦建华石杨姜雷张旭
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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