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Method for realizing on-line production and separation of butanol, acetone and ethanol through adsorbent in-situ adsorption, fermentation, coupling and pervaporation

A technology of pervaporation and adsorbent, which is applied in the biological field to achieve the effects of low fermentation efficiency, improved fermentation efficiency and energy saving

Active Publication Date: 2014-08-27
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no process and related patents for the production and separation of butanol, acetone and ethanol using adsorbent in situ adsorption of ABE fermentation coupled with pervaporation

Method used

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  • Method for realizing on-line production and separation of butanol, acetone and ethanol through adsorbent in-situ adsorption, fermentation, coupling and pervaporation
  • Method for realizing on-line production and separation of butanol, acetone and ethanol through adsorbent in-situ adsorption, fermentation, coupling and pervaporation
  • Method for realizing on-line production and separation of butanol, acetone and ethanol through adsorbent in-situ adsorption, fermentation, coupling and pervaporation

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Experimental program
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Effect test

preparation example Construction

[0042] Preparation of the pervaporation membrane of the present invention: ZSM-5 is first dried at 80° C. for 24 hours. The gel-forming agent base liquid and curing agent in polydimethylsiloxane (PDMS) are mixed at a ratio of 10:1. Mix the ZSM-5 in the specified weight ratio with the PDMS membrane-making solution mixed at a mass ratio of 10:1, and centrifuge at 8000 rpm for 5 minutes for subsequent operations. The follow-up operation is to ultrasonically treat the film-forming solution for 15 minutes to remove the air bubbles in the film-forming solution, then use a scraper to spread the film-forming solution evenly on the glass plate, and put the glass plate with the film-forming solution in an oven at 100°C 3h film formation. Finally, take out the glass plate from the oven, peel off the prepared pervaporation membrane, and fix it in the membrane pool for pervaporation separation operation. The amount of ZSM-5 added is usually 10-100% of the mass of PDMS, preferably 80%.

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Embodiment 1

[0051] A method for on-line separation and purification of butanol in fermentation broth by using adsorbent adsorption fermentation coupled with pervaporation;

[0052] ① if figure 1 As shown, the seed medium was first passed nitrogen in the seed tank for 10 minutes to deoxygenate, then sterilized at 121°C for 30 minutes, cooled to room temperature, inserted into the acetone butanol ethanol production bacteria, and the production bacteria were cultivated to the most active logarithm of growth growth period. The cultivation time until the logarithmic growth phase is 12-18 hours, preferably 15 hours; the cultivation temperature is 30-40°C, preferably 37°C. After the seed culture is completed, it is ready to be inserted into the fermenter.

[0053] ② if figure 1 As shown, first sterilize the fermentation medium at 121°C for 30 minutes, then pass nitrogen gas for 2 hours to remove oxygen, cool to room temperature, turn on the pump I to transfer the seed liquid containing aceton...

Embodiment 2

[0059] A method for on-line separation and purification of butanol in fermentation broth by using adsorbent adsorption fermentation coupled with pervaporation;

[0060] The difference with embodiment 1 is:

[0061] When the fermentation starts, the pump II is not started. When the fermentation progresses to the logarithmic growth phase of the production bacteria, the pump II is started to make the fermentation liquid circulate between the fermenter and the adsorption device, and start the online in-situ adsorption. The fermentation method is feed-fed fermentation. When the glucose concentration in the fermentation broth drops below 10g / L, glucose is added to continue to provide carbon sources for the fermentation until the fermentation is terminated.

[0062] The experimental data are shown in Table 1 and Table 2.

[0063] Summary: After the fermentation, the concentrations of butanol and ABE total solvent were 3.7% (w / v) and 6.2% (w / v), respectively, which were 264.3% and 26...

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Abstract

The invention relates to a method for realizing on-line production and separation of butanol, acetone and ethanol through adsorbent in-situ adsorption, fermentation, coupling and pervaporation, belonging to the technical field of biology. The method comprises the following steps: (1) culturing acetone / butanol / ethanol producing bacteria; (2) performing adsorbent on-line in-situ adsorption on butanol, acetone and ethanol while fermenting; (3) after the adsorption is finished, desorbing, condensing, and recovering to obtain butanol, acetone and ethanol crude products; and (4) performing secondary purification on the butanol, acetone and ethanol crude products through a pervaporation film, condensing vapor permeating the pervaporation film, and recovering to obtain high-purity butanol, acetone and ethanol. According to the invention, the fermentation efficiency is high, and the separation and purification cost is low.

Description

technical field [0001] The invention relates to a method for on-line production and separation of butanol, acetone and ethanol by using an adsorbent in-situ adsorption fermentation coupling pervaporation, and belongs to the field of biotechnology. Background technique [0002] Butanol is an important liquid energy source and chemical, which can be obtained through microbial fermentation, usually using acetone-butanol-ethanol (ABE) fermentation to produce butanol, and at the same time produce acetone and ethanol, as well as a small amount of organic acids. (See non-patent literature for details: Kumar, M, Gayen, K..Developments in biobutanol production: new insights.Applied Energy, 88:1999-2012, 2011). However, the butanol concentration at the end point in the fermentation broth is usually between 1.0 and 2.0 % (w / v), the concentration of acetone is 0.5-1.0% (w / v). In addition, the boiling point of butanol is 117.7°C, which is 100°C higher than the boiling point of water. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/16C12R1/145C12R1/19
CPCY02E50/17Y02E50/10
Inventor 薛闯杨尚天白凤武
Owner DALIAN UNIV OF TECH
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